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Předmět, akademický rok 2018/2019
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Mikrobiologie II - EA0105084
Anglický název: Microbiology II
Zajišťuje: Ústav mikrobiologie (14-510)
Fakulta: Lékařská fakulta v Plzni
Platnost: od 2011
Semestr: zimní
Body: 5
E-Kredity: 5
Způsob provedení zkoušky: zimní s.:
Rozsah, examinace: zimní s.:3/2 Z+Zk [hodiny/týden]
Počet míst: neomezen / neomezen (neurčen)
Minimální obsazenost: neomezen
Stav předmětu: vyučován
Jazyk výuky: angličtina
Způsob výuky: prezenční
Úroveň:  
Poznámka: odhlásit z termínu zkoušky při nesplněné rekvizitě
Garant: RNDr. Karel Fajfrlík, Ph.D.
Vyučující: RNDr. Karel Martínek, Ph.D.
Kategorizace předmětu: Lékařství > Teoretické předměty
Korekvizity : EA0103012, EA0104011, EA0104510
Je korekvizitou pro: EA0107041, EA0108120, EA0107015, EA0110020, EA0108024, EA0108031, EA0108030, EA0108023
Sylabus - angličtina
Poslední úprava: Kateřina Babincová (15.02.2012)

Microbiology, General Medicine - Syllabus

 First semester:          practical courses - 2 hrs/week

Second semester:      lectures - 3 hrs/week

                                   practical courses - 2 hrs/week

 Lectures, Clinical microbiology:

 1.      Introduction, history of clinical microbiology, evolution of microbes, bacterial genetics (viruses, bacteria, parasites, mechanisms of horizontal gene transfer - transformation, transduction, conjugation, specific genetic elements - insertion sequences, transposons, integrons, etc.) 

2.      Principles of general microbiology, factors responsible for virulence, pathogenesis of infectious diseases (structure of virus, virus living cycle, basic knowledge of immunity response, barriers, bacterial toxins, adhesins, mechanisms of escape against immunity, etc.)

3.      Review of clinically important  G+ bacteria (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, antibiotic therapy; Streptococcus spp., Enterococcus spp., Staphylococcus spp., Bacillus spp., Corynebacterium spp., etc.)

4.      Review of clinically important  G- bacteria  (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, antibiotic therapy; Neisseria spp., Haemophilus spp., Enterobacteriaceae, Vibrio spp., Campylobacter spp., Helicobacter spp., Pseudomonas and other non-fermenting rods, Legionella spp.)

5.      Review of clinically important  bacteria (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, antibiotic therapy; Mycobacterium spp., Nocardia spp., Actinomyces spp., Chlamydia spp., Mycoplasma spp., Rickettsia spp., anaerobic bacteria - review of the most important species, e.g. Clostridium difficile, C. perfringens, C. tetani, C. botulinum, Peptostreptococcus spp., Fusobacterium spp.)

6.      Review of clinically important parasites 1 (physiology of parasite, living cycle, vectors, diseases, diagnostics, treatment; protozoology, Trypanosoma spp., Leishmania spp., Trichomonas vaginalis, Giardia intestinalis, Entamobea histolytica, Plasmodium spp., Toxoplasma gondii, Balantidium coli, Cryptosporidium parvum)

7.      Review of clinically important parasites 2 (physiology of parasite, living cycle, vectors, diseases, diagnostics, treatment; helminthology, medical entomology, Fascilopsis buski, Paragonimus westermani, Clonorchis sinensis, Schistosoma, spp., Taenia spp., Echinococcus spp., Hymenolepis nana, Nematodes)

8.      Review of clinically important fungi (characteristics, factors responsible for virulence, diseases, diagnostics, epidemiology, therapy; Candida spp., Craptococcus spp., Aspergillus spp., Penicillium spp., dermatomycoses, Hystoplasma spp., Pneumocystis jirovecii)

9.      Antibiotics (basic groups of antibiotics, mechanism of action, molecules; beta-lactams, glycopeptides, polymyxins, amynoglykosides, quinolones, sulfonamides, chloramphenicol, etc.)

10.  Mechanisms and epidemiology of antibiotic resistance (mechanisms of bacterial resistence, clonal spread of resistant bacteria, horizontal transfer of genes - mobilisation of genes, mobile genetic elements, integrons, co-resistnace, cross-resistance) 

11.  Hospital-acquired infections (definition, examples of the most important pathogens, resistence bacteria as a causative agents of hospital-acquired infections - MRSA, VRE, resistant Gram-negative rods - production of extended-spectrum beta-lactamases, carbapenemases)

12.  Review of viral diseases 1 (structure of virus, pathogenesis, epidemiology, diagnostics, prevention, therapy; papillomaviruses, polyomaviruses, adenovirus, herpesviruses, poxviruses, picornaviruses, coronaviruses,  paramyxoviruses, influenza virus, rhabdoviruses, filoviruses, reoviruses, togaviruses and flaviviruses, togaviruses, bunyaviridae) 

13.  Review of viral diseases  2 (structure of virus, pathogenesis, epidemiology, diagnostics, prevention, therapy; retroviruses - HIV, hepatitis viruses)

14.  Indication, limitations, and interpretation of microbiological diagnostics methods

 

 

 Microbiology exercises for general medicine I. (summer semester)

 1) Introduction - safety, classification of microbes, characterization of main groups of microbes

2) Dizinfection and sterilization - fyzical and chemical methods  and agents, indication and quality control, laboratory equipment and its application

3) Cultivation I. - specimens collection, processing and storage, culture media characteristics

4) Cultivation II. - seeding of culture media, characteristics of  microbial growth

5) Microscopy I. - Gram stain, bacterial morphology

6) Microscopy II. - Acid-fast stain, negative stain, slides of clinical specimens, host cells identification

7) Cultivation III. - Biochemical and additional tests

8) Cultivation IV. - Antibiotic susceptibility testing

9) Immunological methods I.- terms definition, polyclonal and monoclonal antibodies charteristics, production and application,

10)  Immunological methods II. - Principles of direct and indirect immunological methods

11) Immunological methods III. - Main assays, its characteristics, application and limits in diagnostics

12) Identification of medically important microbes - main groups of  microbes in cultures and microscopy slides

 

Microbiology exercises for general medicine II. (winter semester)

 1) Normal microflora, laboratory diagnoses of anaerobes - normal microflora on culture media and microscopy slides, primocultures of various sites with fyziological colonization, Methods of anaerobic cultivation, genus Clostridium, non-spore-forming anaerobes

2) Laboratory diagnosis of skin mucosae and soft tissue infections, sexually transmited diseases -diagnostic strategies,  collection of specimens, causative agent  detection and identification: Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Clostridium sp. (histolytic), non-spore-forming anaerobes, diagnostic methods of bacterial STD: Treponema pallidum, Neisseria gonorrhoeae, Chlamydia trachomatis

3) Laboratory diagnosis of intestinal and urinary tract infections - diagnostic strategies,  collection of specimens, causative agent  detection and identification: Salmonella sp., Shigella sp., Yersinia enterocolitica, Escherichia coli, Vibrio cholera, Campylobacter jejuni, Helicobacter pylori, Proteus sp., Klebsiella sp., Staphylococcus sp., Enterococcus sp.

4) Laboratory diagnosis of respiratory tract infections - diagnostic strategies,  collection of specimens, causative agent  detection and identification: Streptococcus pyogenes, Streptococcus pneumonie, Haemophilus influenzae, Bordetella pertussis, Pseudomonas aeruginosa,  Chlamydia pneumoniae, Mycoplasma pneumoniae, Mycobacterium tuberculosis,  Corynebacterium diphteriae

5) Laboratory diagnosis of zoonotic diseases - diagnostic strategies, collection of specimens, causative agent  detection and identification: Leptospira sp., Borrelia burgdorferi, Chlamydia psittaci, Francisella tularensis, Pasteurella multocida, Listeria  monocytogenes, Salmonella sp., non-tuberculous mycobacteria

6) Laboratory diagnosis of CNS infections, sepsis - diagnostic strategies, collection of specimens, causative agent detection and identification: Haemophilus influenzae, Streptococcus pneumoniae, Neisseria meningitides, Streptococcus agalactiae, haemocultivation - collection of blood specimen, set up rules

7) Antimicrobial therapy - case reports

8) Laboratory diagnosis  of parasites I. -  coprology - methods of concentration, blood smears, vaginal smears, protozoan parasites -  diagnostic strategies, collection of specimens, causative agent detection and identification: Trichomonas vaginalis, Giardia intestinalis, Leishmania sp., Entamoeba sp., Toxoplasma gondii, Plasmodium falciparum, Plasmodium vivax,

9) Laboratory diagnosis  of parasites II. - parasitic helminth and parasitic arthropods -   diagnostic strategies, collection of specimens, causative agent detection and identification: Ascaris lumbricoides, Enterobius vermicularis, Taenia saginata, Toxocara sp., Trichuris trichiura, Pediculus humanus, Ixodes ricinus

10) Laboratory diagnosis of fungi I. Specific diagnostic methods in mycology. Yeasts-diagnostic strategies, collection of specimens, causative agent detection and identification: Candida albicans, Candida sp., Cryptococcus neoformans, Malassezia furfur

11) Laboratory diagnosis of fungi II. Molds - diagnostic strategies, collection of specimens, causative agent detection and identification: Aspergillus sp., Mucor sp., Trichophyton sp., Microsporum sp.,

12) Laboratory diagnosis of viruses I. Specific diagnostic methods in virology. Diagnostic strategies, collection of specimens, causative agent detection and identification.

13) Laboratory  diagnosis of viruses II Excursion to the Department of Virology

 

 
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