Last update: RNDr. Jana Rubešová, Ph.D. (05.05.2021)
The aim of this practical course is to introduce students to both common and modern instrumental biochemical methods. Besides commonly used techniques, such as extraction of compounds from natural material, UV/VIS absorption spectrophotometry, spectrofluorimetry, chromatographic techniques (HPLC, FPLC, ion exchange, gel permeation and affinity chromatography), electromigration methods (agarose electrophoresis, SDS-PAGE, Western blot - electroblotting with immunodetection), and PCR amplification of DNA, as well as basic bioinformatic training, practical utilisation of biochemical information sources, work with scientific literature and evaluation of experimental results. The course is finished by introduction to principles of text structuring and formal requirements relevant to scientific literature (i.e. bachelor thesis or scientific publications) and subsequent writing of a final report for one given practical task in a form of small scientific work, its timely submission and oral discussion.
The practical course runs on two days in one week, i.e. both on Mondays and Thursdays. The task schedule is available upon registration in Moodle and will be created after the first introductory meeting at the beginning of the course where students pass and sign the safety training and also divide into working groups (two people) - the personal attendance at this meeting is mandatory!
Theoretical introduction to all used techniques, supervision of the exercises and discussion of the obtained results is provided in English to all Erasmus students. The written final report is accepted in English.
Literature -
Last update: doc. RNDr. Václav Martínek, Ph.D. (30.09.2023)
Manuals for each method and examples of protocols are available in Moodle.
Last update: RNDr. Jana Rubešová, Ph.D. (05.05.2021)
Doporučená literatura:
Krajhanzl A, Hladík J et al.: Biochemické metody. Návody k praktickým cvičením. UK Praha 1991.
Anzenbacher P, Kovář J: Metody chemického výzkukmu pro biochemiky, MŠ Praha, 1986
Requirements to the exam -
Last update: RNDr. Jana Rubešová, Ph.D. (05.05.2021)
Credit: successful work in the laboratory on all given laboratory tasks, delivery of the final report in required quality and in time, oral discussion of the report.
Last update: RNDr. Jana Rubešová, Ph.D. (05.05.2021)
Zápočet: úspěšné absolvování všech zadaných úloh, vypracování přiděleného protokolu v odpovídající kvalitě, jeho včasné odevzdání a diskuse
Syllabus
Last update: doc. RNDr. Václav Martínek, Ph.D. (30.09.2023)
Tasks:
INF/PYM - Basics of bioinformatics and Visualization of biomacromolecules in PyMol software
IEX - Separation of selected proteins by ion exchange chromatography on FPLC
IMAC & WB - Isolation, purification, electrotransfer and immunodetection of recombinant protein (e.g. TEV protease or green fluorescent protein)
X - protein crystallization and methods of biomacromolecules' structure determination
PCR - Polymerase chain reaction of DNA
LIT - Training in work with scientific literature
The course is finished by delivering a short report from one given lab task in a form of short scientific writing.
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The course was innovated within the project ESF for University II at Charles University, reg. No .: CZ.02.2.69/0.0/0.0/18_056/0013322
Entry requirements -
Last update: doc. RNDr. Václav Martínek, Ph.D. (30.09.2023)
The laboratory course is suitable for students of the 2rd and 3rd year of bachelor's studies in chemistry or biological disciplines. Most tasks are focused on advanced instrumental biochemical methods, so we assume that participants are equipped with knowledge of basic chemical and biochemical techniques in addition to the theoretical understanding of biochemistry. And that they have already successfully completed the introductory "Biochemical Laboratory" courses or equivalent subjects, an entrance test can verify the student's knowledge.
Last update: doc. RNDr. Václav Martínek, Ph.D. (10.01.2022)
Praktikum je určeno pro studenty 2. a 3. ročníků bakalářského studia chemických popř. biologických oborů. Vzhledem k tomu, že se zaměřuje na pokročilé instrumentální biochemické metody, předpokládáme, že účastníci jsou kromě teoretické znalosti biochemie vybaveni také znalostmi základních chemických a biochemických metod. Tedy, že již úspěšně absolvovali Praktikum z laboratorní techniky a také Biochemické praktikum (MC250C31N), popř. ekvivalentní předměty, což může být ověřeno vstupním testem.
V případě zavedení omezení např. v souvislosti se zavedením protiepidemických opatření může dojít ke změně podmínek.
