SubjectsSubjects(version: 901)
Course, academic year 2022/2023
  
Advanced Practical Biochemistry for KATA - MC250C03E
Title: Advanced Practical Biochemistry for KATA
Czech title: Pokročilé praktikum z biochemie pro KATA
Guaranteed by: Department of Biochemistry (31-250)
Faculty: Faculty of Science
Actual: from 2021
Semester: summer
E-Credits: 4
Examination process: summer s.:
Hours per week, examination: summer s.:0/3 C [hours/week]
Capacity: 48
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: English
Is provided by: MC250C03
Explanation: in English
Additional information: https://dl2.cuni.cz/course/search.php?search=MC250C03
Note: enabled for web enrollment
Guarantor: RNDr. Ondřej Vaněk, Ph.D.
doc. RNDr. Václav Martínek, Ph.D.
Class: Vysokokapacitní centrifuga s příslušenstvím
Stolní centrifuga s příslušenstvím
Původní předmět
Co-requisite : {At least one of the following subjects: MC250C42N, MC250C31N, MC250C32, MB150C04, MC250C35 MC250C35N, MC250C32N}
Incompatibility : MC250C03, MC250C17A, MC250C17E
Is incompatible with: MC250C17E, MC250C17A, MC250C03
Opinion survey results   Examination dates   Schedule   
Annotation
Last update: RNDr. Jana Rubešová, Ph.D. (05.05.2021)
The aim of this practical course is to introduce students to both common and modern instrumental biochemical methods. Besides commonly used techniques, such as extraction of compounds from natural material, UV/VIS absorption spectrophotometry, spectrofluorimetry, chromatographic techniques (HPLC, FPLC, ion exchange, gel permeation and affinity chromatography), electromigration methods (agarose electrophoresis, SDS-PAGE, Western blot - electroblotting with immunodetection), and PCR amplification of DNA, as well as basic bioinformatic training, practical utilisation of biochemical information sources, work with scientific literature and evaluation of experimental results. The course is finished by introduction to principles of text structuring and formal requirements relevant to scientific literature (i.e. bachelor thesis or scientific publications) and subsequent writing of a final report for one given practical task in a form of small scientific work, its timely submission and oral discussion.

The practical course runs on two days in one week, i.e. both on Mondays and Thursdays. The task schedule is available upon registration in Moodle and will be created after the first introductory meeting at the beginning of the course where students pass and sign the safety training and also divide into working groups (two people) - the personal attendance at this meeting is mandatory!

Theoretical introduction to all used techniques, supervision of the exercises and discussion of the obtained results is provided in English to all Erasmus students. The written final report is accepted in English.
Literature -
Last update: doc. RNDr. Václav Martínek, Ph.D. (10.01.2022)

No specific literature is required, but we strongly recommend reading the material, manuals, and handbooks of each biochemical laboratory method provided in Moodle.

Requirements to the exam -
Last update: RNDr. Jana Rubešová, Ph.D. (05.05.2021)

Credit: successful work in the laboratory on all given laboratory tasks, delivery of the final report in required quality and in time, oral discussion of the report.

Syllabus
Last update: doc. RNDr. Václav Martínek, Ph.D. (10.01.2022)

The course includes comprehensive practical training in advanced biochemical laboratory methods, visualization of biomolecules, training in working with scientific literature, citation manager, and writing a short thesis.

Practical tasks include:

  • INF/PYM - Basics of bioinformatics and visualization of biomacromolecules in PyMol software
  • IEX - Separation of proteins using ion-exchange chromatography and gel filtration on FPLC
  • IMAC & WB - Isolation, purification, electrotransfer, and immunodetection of a protein produced via heterologous expression
  • X - protein crystallization and methods of biomacromolecules' structure determination
  • PCR - Polymerase chain reaction of DNA, rtPCR
  • LIT - Training in work with scientific literature

The course is finished by writing a short report from one given lab task in a form of short scientific report.

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The course was innovated within the project ESF for University II at Charles University, reg. No .: CZ.02.2.69/0.0/0.0/18_056/0013322

Entry requirements -
Last update: doc. RNDr. Václav Martínek, Ph.D. (10.01.2022)

The laboratory and practical tasks are intended for students of the 2nd and 3rd year of bachelor's studies in chemistry or biological disciplines. Given that it focuses on advanced instrumental biochemical methods, we assume that participants already acquired knowledge of basic chemical and biochemical methods and have a basic theoretical background in biochemistry. That is, they have already successfully completed courses Laboratory Technology Laboratory and also the Biochemical Laboratory (MC250C31N), or equivalent subjects, which can be verified by an entrance test.

If restrictions are imposed, for example in connection with the introduction of anti-epidemic measures, the conditions may change.

 
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