SubjectsSubjects(version: 953)
Course, academic year 2023/2024
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Biology of the Cell - MB150P31
Title: Biologie buňky
Czech title: Biologie buňky
Guaranteed by: Department of Cell Biology (31-151)
Faculty: Faculty of Science
Actual: from 2019 to 2023
Semester: winter
E-Credits: 6
Examination process: winter s.:
Hours per week, examination: winter s.:4/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Note: enabled for web enrollment
Guarantor: doc. RNDr. František Půta, CSc.
Teacher(s): prof. RNDr. Jan Černý, Ph.D.
RNDr. Lenka Libusová, Ph.D.
doc. RNDr. František Půta, CSc.
Incompatibility : MB151P95
Is incompatible with: MB151P95, MB150P73, MB150P73G
Is pre-requisite for: MB130P36
Is complex co-requisite for: MB150C28, MB150P04A
In complex pre-requisite: MB150P22, MB152P02
Annotation -
Please note, the lectures are given in czech language only.

The course provides the essential information of cell biology (cell organization, infrastructure, function of the particular structures, and research methodology). The arrangement of the lecture follows modern textbooks, Alberts et al.: Molecular Biology of the Cell či Lodish et al.: Molecular Cell Biology.
Last update: PUTA (21.03.2006)
Literature -

Alberts et al.: Molecular Biology of the Cell. 3rd ed., 1994 (ISBN 0-8153-1919-4), 4th ed., 2002 (ISBN 0-8153-4072-9).
Lodish et al.: Molecular Cell Biology. 3rd ed., 1995 (ISBN 0-7167-2380), 4th ed., 2000, 5th ed., 2004 (ISBN 0-7167-4366-3).
Alberts et al.: Základy buněčné biologie - Úvod do molekulární biologie buňky (orig. Essential Cell Biology. Garland Publishing, Inc. NY, 1st edition, 1997) 2001 (ISBN 80-902906-0-4).
Alberts et al.: Essential Cell Biology. 1st ed., 1998 (ISBN 0-8153-2045-0 Hardcover,
ISBN 0-8153-2971-7 Paperback). 2nd ed. 2003 (ISBN 0-8153-3480-X).
Pollard T.D.: Cell Biology. 1st ed., 2002 (ISBN 0-7216-3997-6).
Kubišta: Buněčné základy životních dějů. Sciencia, 1998 (ISBN 80-7183-109-3).

Last update: Půta František, doc. RNDr., CSc. (25.02.2021)
Requirements to the exam -

The exam will require knowledge, concepts and their relationships, traceable in the recommended literature, in the scope of the lectured material. High school knowledge is  assumed automatically.

The exam is organized as a written test; the student has the option of an oral examination after meeting the specified point limit. In the test, you choose the correct answers from four given answers, where any number of answers can be correct or incorrect (multiple choice test). If you mark all the correct and only the correct answers, you get 5 points, if we agree in three cases you get 3 points. Match in one or no answer is not evaluated. The "good" grade limit is around fifty percent of points (max. 150). There are 60 minutes of pure time for the test.

Last update: Půta František, doc. RNDr., CSc. (25.02.2021)
Syllabus -

1. Internal organization of the cell, construction and function of cell compartments - cell theory, a cell in a light microscope, subcellular structures and compartmental arrangement of eukaryotic cells, comparison of bacterial cells, archaea and eukaryotes, types of eukaryotic cells, differentiation of multicellular organism cells, types and structure interaction with the host cell.
2. Proteins and their functions in the cell - structural hierarchy of proteins, motifs and domains, protein-protein and other interactions, supramolecular complexes - ribosome, proteasome, spliceosome, etc., synthesis and distribution of proteins in the cell, folding and participation of chaperones, cleavage and modifications, protein turnover.
3. Membranes and transport - biomembrane structure, composition, biogenesis, and function of a lipid bilayer, function of membrane proteins, receptors, membrane potential, transmembrane transport of substances, types of transport.
4. Internal membrane structures and transport - endoplasmic reticulum, Golgi complex - biosynthesis and transport, vesicular transport, endo- and exocytosis, lysosome, vacuoles, peroxisome, hydrogen transfer.
5. Semiautonomous organelles - mitochondria, chloroplast - structure, semiautonomy, proofs of origin, contribution to the energy balance of the cell.
6. Anatomy and function of the cell nucleus - structure of the nucleus - nuclear envelope, nucleoskeleton, organization of genetic information - chromosome, chromatin, principles of genetic preservation and utilization, information, nuclear transport of macromolecules.
7. Communication between cells, multicellularity - types of signaling - endocrine, synaptic, paracrine, contact, receptors - membrane and nuclear, intracellular signal transporters, molecular switches, signaling cascades and their integration.
8. Shape control and cell movement - cytoskeleton, microtubules, microfilaments, intermediate filaments, molecular motors, and other associated proteins, the participation of plasma membrane and extracellular matrix proteins, cell junctions, cell interactions with the extracellular matrix, types of cell movement, and their use in multicellular organisms
9. Cell division and cell cycle - cell proliferation, interphase, nuclear division and division apparatus, cytokinesis, cell cycle control, cell growth regulation, apoptosis, tumor growth.
10. Cells of the immune system and immunity - the role of cells in the immune defense system, multicellular organism, receptors, antibodies, MHC and other components, principles of B and T cell activation, recombination and mutation as a basis for immunoglobulin variability, clonal selection, inflammation.

Last update: Půta František, doc. RNDr., CSc. (25.02.2021)
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