SubjectsSubjects(version: 953)
Course, academic year 2023/2024
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Instrumental Methods - GF301
Title: Instrumentální metody
Guaranteed by: Department of Analytical Chemistry (16-16140)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2023
Semester: summer
Points: 0
E-Credits: 6
Examination process: summer s.:combined
Hours per week, examination: summer s.:14/56, C+Ex [HS]
Capacity: unlimited / 270 (unknown)
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: deregister from the exam date if a requisite was not fulfilled
course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: doc. PharmDr. Hana Sklenářová, Ph.D.
Comes under: 2.ročník 2023/24 Farmacie
Co-requisite : GF300
Interchangeability : GF274
Is co-requisite for: GF313, GF302, GF314
Is pre-requisite for: GF385, GF306
Is interchangeable with: GF274
Annotation -
The aim of the subject is to provide the students with firm and sufficiently wide theoretical and methodological fundamentals for pursuing (in a rational way) instrumental analysis of inorganic and organic substances. The teaching is focused on the explanation of theoretical aspects of instrumental methods and putting the accent on problem-solving tuition. In practical training individual approach of students to solving analytical problems is required.
Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (18.09.2023)
Course completion requirements -

Practical course

1.      Participation in all practical classes – (missing practical classes must be revisited or it can be substituted if permitted by the teacher)

2.      Unexcused absence (without confirmation of illness from a doctor) is solved in the form of paid extra classes

3.      Submission of all reports of practical exercises

4.      Minimum 66% of points for correct results in practical tasks and for correct answers in the monitoring and credit tests



1.         The exam in Instrumental methods is organized in terms presented in the Study information system, the time of exam is always specified. Simple calculator is needed for written exam. Evaluation is carried out directly after evaluation of the test.

2.         The exam is organized together with the subject Analytical chemistry. The form of exam is oral with previous test.

3.         For the exam, credits from both practical classes are needed.

4.         Exam organization:

The first term – written test + oral examination

The second term - written test + oral examination

The third term – oral examination

5.         Exam scope for the third term is specified in the Study materials – Study KACH – Instrumental methods

Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (18.09.2023)
Literature - Czech


  • Karlíček, Rolf, Polášek, Miroslav, Pospíšilová, Marie, Solich, Petr. Analytická chemie pro farmaceuty. Praha: Karolinum, 2001, 281 s. ISBN 80-246-0348-9.
  • Karlíček, Rolf, Dolejšová, Jana, Polášek, Miroslav, Pospíšilová, Marie, Solich, Petr. Analytická chemie : návody do cvičení z kvalitativní anorganické a organické analýzy. Praha: Karolinum, 2000, 89 s. ISBN 80-246-0121-4.
  • Karlíček, Rolf. Analytická chemie : návody do cvičení z kvantitativní analýzy. Praha: Univerzita Karlova, 1995, 163 s. ISBN 80-7184-023-8.
  • Karlíček, Rolf, Dolejšová, Jana, Polášek, Miroslav, Pospíšilová, Marie, Solich, Petr. Analytická chemie : protokoly ke kvalitativní anorganické a organické analýze a ke kvalitativní analýze. Praha: Nakladatelství Karolinum, 2000, 122 s. ISBN 80-246-0160-5.


  • Rücker, G. Neugebauer, M. Willems, G. G.. Instrumentelle pharmazeutische Analytik : Lehrbuch zu spektroskopischen, chromatographischen und elektrochemischen Analysenmethoden; mit 70 Tabellen. Stuttgart: Wissenschaftliche Vlg mbH, 1988, 428 s. ISBN 3-8047-1015-8.
  • Robinson, James W., Frame, Eileen M. Skelly Frame, George M.. Undergraduate instrumental analysis. New York: M. Dekker, 2005, 1079 s. ISBN 0-8247-5359-3.
  • Klouda, Pavel. Moderní analytické metody. Ostrava: Pavel Klouda, 2003, 132 s. ISBN 80-86369-07-2.

Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (18.09.2023)
Syllabus -

Syllabus – Instrumental methods

Introduction to instrumental methods

  • Instrumental methods, their advantages, drawbacks, classification of instrumental methods, their application in qualitative and quantitative analysis.

Optical methods

  • Basic optical methods, interaction of radiation with particulate matter, classification of optical methods, their principles and scope, fundamentals of luminescence analysis, practical use of optical methods.

  • Spectrophotometry in UV spectral region, types of electron transfer, light absorption of organic compounds, absorption spectrum, absorption band, absorption coefficient, double-beam spectrophotometers, diode-array detector, analytical applications – identification, purity of substances, quantitative assay, calibration curve, analysis of multi-component systems.

  • Infra-red spectrophotometry, types of vibrations, characteristic vibrations of functional groups, absorption spectrum, absorption bands, instrumentation, measuring techniques, analytical applications – interpretation of IR spectra, identification, purity, structure of organic compounds.

  • Flame photometry, refractometry, polarimetry, principles, and analytical applications.

  • Raman spectrometry - application of identification o organic substances.

Electroanalytical methods

  • Basic electroanalytical methods, their classification and principles. Potentiometry, principles, instrumentation, selection of electrode system, ion-selective electrodes, potentiometric titrations and their evaluation, analytical applications. Conductimetry, principles, conductimetric titrations and other applications.

  • Voltammetry and polarography, principles, instrumentation, qualitative and quantitative analysis.

Radioanalytical methods

  • Basic radioanalytical methods, their classification, principles, applications.

Separation methods

  • Basic separation methods, classification according to the principle of separation. Chromatographic methods and their classification.

  • Gas chromatography, instrumentation, analytical applications.

  • Liquid chromatography in planar and packed-bed arrangement. High-performance liquid chromatography (HPLC). Thin-layer chromatography (TLC, HPTLC). Paper chromatography. Instrumentation and analytical applications.

Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (18.09.2023)
Learning resources - Czech

E-learning Moodle: Instrumentální metody

Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (18.09.2023)
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