Poslední úprava: prof. RNDr. Dalibor Šatínský, Ph.D. (01.03.2018)
1) Definition of basic concepts
Mass spectrometry, important historical milestones, basic components of the mass spectrometer, specifics of the laboratory for mass spectrometry, mass spectrum, isotopic representation of elements in nature
2) Ionization techniques in mass spectrometry
Theory, History, and Development of Separation Techniques with Mass Spectrometry
Types of ionization techniques - soft, hard, further distribution of ionization techniques.
EI, CI - detailed principle and possibilities of use.
3) Ionization techniques at atmospheric pressure
ESI, APCI, APPI - detailed principle and usage possibilities.
4) Ambient ionization techniques
Trends in the development of ionization techniques. DESI, DART, REIMS - detailed principle and possibilities of utilization, imaging, MALDI.
5) Analyzers in mass spectrometry
Definition of parameters for mass analyzers - sensitivity, resolution and accuracy of matter. Different types of weight analyzers. Flight analyzers - quadrupole, TOF, magnetic
Trap analyzers: ion traps, orbital trap, ICR
Tandem mass spectrometry. Hybrid mass analyzers (Q-TOF, Q-IT ...)
6) Types of records in mass spectrometry
MS mode: MS scan, SIM, MS / MS mode: SRM, scan of product and precursor ions, scan of neutral losses, separation by ion mobility
7) Combination of mass spectrometry with separation techniques
HPLC-MS, SFC-MS, GC-MS, CZE-MS - types of ionization techniques, interface types, practical realization
8) Approaches for quantification of substances using mass spectrometry
External and internal standard method for mass spectrometry. Standard addition method. Correct choice of internal standard. Matrix effects. Targeted and non-targeted analysis.
9) Interpretation of mass spectra - hard ionization techniques
Rules for interpreting the EI Spectrum.
10) Approaches for identifying substances using mass spectrometry
Identification of substances in MS and HRMS. Database, Clarification of Substance Structures.
Calibration of masses, measurement of spectra in HRMS.
Interpretation of mass spectra - soft ionization techniques
11) Application of mass spectrometry - small molecules
Examples of MS utilization in the field of pharmaceutical analysis, bioanalysis, doping control
12) Application of mass spectrometry - proteomics
The specifics of MS utilization in protein biomolecules analysis