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This course is taught in English for Erasmus and PhD students. New trends and techniques in the fields of atomic and molecular spectroscopy and mass spectrometry are discussed including their applications. Also advanced approaches to sample preparation, analyte introduction including preconcentration strategies are presented. Lectures are accompanied by excursions to selected laboratories whenever possible. Practical demonstrations of analytical measurements using state-of-the-art spectrometric instrumentation will be shown. The series of lectures is given by experienced researchers from Czech universities and Czech Academy of Sciences. Basic knowledge of fundamental spectroscopic techniques is expected. Active participation of the students is expected with maximum absence of 20%.
The course starts on Wednesday 26 February 2025. The schedule of lectures and excursions will be specified after agreement regarding the students' time availability during the first week of semester. Final programme for summer semester 2025: Date Lecturer Topic Institution 26.2. RNDr. Jan Kratzer, Ph.D. Introduction to atomic, molecular and mass spectrometry IAC CAS 5.3. MSc. Gilberto Coelho, Ph.D. Microwave-induced combustion: An advanced and robust sample preparation method IAC CAS 12.3. RNDr. Jan Kratzer, Ph.D. Preconcentration techniques compatible with trace element analysis and atomic spectrometry IAC CAS 19.3. RNDr. Jan Kratzer, Ph.D. Laser ablation ICP-MS (LA-ICP-MS) and laser induced breakdown spectroscopy (LIBS) IAC CAS 26.3. Prof. Patrik Španěl Soft chemical ionization mass spectrometry (SIFT-MS, SESI-MS)+ applications to trace gas analysis JH IPC CAS 28.3. RNDr. Oldřich Benada, CSc. Electron microscopy and X-ray microanalysis and their applications in nanoparticles research MBU CAS 2.4. RNDr. Tomáš Matoušek, Ph.D. Elemental speciation analysis IAC CAS 9.4. RNDr. Václav Červený, Ph.D. Spectrometric analysis without sample preparation FS CU 11.4. Assoc. Prof. Pavel Dvořák, Ph.D. Photoluminiscence FS MU 16.4. RNDr. Jan Kratzer, Ph.D. Volatile species generation as a sample introduction technique IAC CAS 23.4. Assoc. Prof. Antonín Kaňa, Ph.D. From proof of concept to the routine use of single-particle ICP-MS UCT IAC CAS – Institute of Analytical Chemistry, Czech Academy of Sciences FS CU – Faculty of Science, Charles University, Prague FS MU – Faculty of Science, Masaryk University, Brno UCT – University of Chemistry and Technology Prague JH IPC CAS – J. Heyrovský Institute of Physical Chemistry, Czech Academy of Sciences MBU CAS – Institute of Microbiology, Czech Academy of Sciences Poslední úprava: Kratzer Jan, RNDr., Ph.D. (18.03.2025)
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Handbook of High-resolution Spectroscopy. Wiley 2011 Poslední úprava: Kratzer Jan, RNDr., Ph.D. (14.02.2024)
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This subject will be finished by an oral exam. The exam will cover all topics lectured during the session. Active participation in the lectures and excursions (maximum absence 20 %) is required. Poslední úprava: Kratzer Jan, RNDr., Ph.D. (14.02.2024)
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Advanced spectrometric techniques used in atomic, molecular and mass spectrometry are discussed including their principles and applications. This year following techniques will be covered: Raman spectroscopy Raman optical activity (ROA) Raman microscopy Vibrational circular dichroism (VCD) Single particle ICP-MS (sp-ICP-MS) Speciation analysis by ICP-MS Photoluminiscence Laser ablation ICP-MS (LA-ICP-MS) and laser induced breakdown spectroscopy (LIBS) Electron microscopy (TEM, SEM) X-ray microanalysis (EDS) Selected ion flow tube mass spectrometry (SIFT-MS) and secondary electrospray ionization mass spectrometry (SESI-MS) Sample preparation (microwave induced combustion) Analyte preconcentration strategies Poslední úprava: Kratzer Jan, RNDr., Ph.D. (14.02.2024)
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After completing the course, the student will be able to: 1) explain the general terms, processes and equations used in spectroscopy - explain differences in atomic, molecular and mass spectra - use actively and correctly the equations to express wavelength and energy of photons - sketch schemes of following processes: photon absorption, emission, fluorescence and ionization - distinguish spectrometric methods with and without mutual energy exchange between radiation and matter - categorize spectrometric methods according to the energy of radiation measured
2) explain the importance of sample preparation and evaluate mutual compatibility of this step with subsequent spectrometric detection - give examples of modern and advanced approaches to analyte separation from matrix and/or analyte preconcentration - give examples of conventional and advanced sample digestion methods - give examples of spectrometric methods with no/minimum need for sample treatment - distinguish between total element determination and speciation analysis including an explanation of the specifics of sample preparation
3) explain basic principle and give examples of applications of advanced spectrometric techniques employed for - trace element and speciation analysis such as single particle (sp)-ICP-MS, laser ablation (LA)-ICP-MS, laser induced breakdown spectroscopy (LIBS), X-ray microanalysis - surface analysis by electron microscopy (EM): explain differences between measurement concepts, sample preparation and applications in scanning (SEM) and transmission (TEM) electron microscopy - analysis of biomolecules and pharmaceuticals: chiroptical spectroscopic methods, photoluminiscence - ambient ionization mass spectrometry for identification and quantification of (bio)molecules - gas phase analysis for clinical, food safety and hi-tech industry samples: selected ion flow tube mass spectrometry (SIFT-MS), secondary electrospray ionization (SESI) MS Poslední úprava: Kratzer Jan, RNDr., Ph.D. (30.09.2024)
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