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The course Biological Techniques explain experimental approaches and methodologies of modern biology.
Seminal scientific discoveries from the first three biological lectures of the Science curriculum will be put in context with the particular research approach. Biological Techniques expands understanding of the biological concepts and facts in a practical way necessary for successful research career. The list of covered techniques will correspond to the broad diversity of biological disciplines, with the emphasis to reach state of art achievements. All lecturers are active scientists, specialists in a field they teach. The course Biological Techniques forms the integral unit with three prerequisite courses (From molecules to cells, From cells to organisms and On the Evolution and Ecology). The course is built from topical blocks (see Syllabus) each of them consisting of lecture (3h) and workshop focused on primary literature as a Q&A session (2h). Poslední úprava: Půta František, doc. RNDr., CSc. (06.02.2022)
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1. Understanding Light Microscopy (RMS - Royal Microscopical Society) 1st Edition, ISBN-13: 978-0470973752, 2019 Poslední úprava: Šebková Nataša, RNDr., Ph.D. (31.05.2022)
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Final mark is based on the oral examination (67%) and results of tests taken during the course (33%). Oral examination takes place during the examination period and students must first obtain the evaluation for Q&A sessions, workshops and take-home exercises. Poslední úprava: Půta František, doc. RNDr., CSc. (06.02.2022)
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Introduction to biological research (ethics, observation and experimentation, hypothesis driven research, correct interpretation of the primary data) Seeing is believing - principles of visualisation techniques: limitations, types (AFM, SEM, TEM, light-based), applications, data mining approaches, quantitation, 3D, life imaging, whole-animal imaging, labelling techniques, image analysis Structural biology Computational biology Genetic analysis (sequencing, transcriptomics, epigenomics, study of polymorphisms) Bioinformatics Characterization of protein structure and function - proteomics, NMR, X-ray… Single cell techniques Gene modifications and phenogenomics Application of fluorescent proteins and probes Cladistics Flow cytometry and usage of antibodies Neurophysiological techniques Poslední úprava: Půta František, doc. RNDr., CSc. (06.02.2022)
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