The final examination on Medical Chemistry and Biochemistry consists of two parts: written and oral. At least 57% of obtainable points must be reached in the written test in order to proceed to the oral part of the final examination. This requirement shall not be applied in case of the third exam attempt. Students who pass the written test but fail in the oral part will not write the test again in the subsequent exam attempts. If a student fails to answer a partial question at the exam, it is sufficient as a cause for termination of examination and classification of the whole exam as ‘failed’.
A student can take an examination in a subject for three times as a maximum, i.e. the student is entitled to two re-examinations, and no extraordinary terms beyond that are allowed. In case of the second re-examination in a mandatory or elective subject the student is examined by a committee consisting of at least two examiners. If the internal faculty regulations (Article 19, Paragraph 2), permit repeated registration for the subject, the examination by the committee shall be applied only to the second re-examination in case of the repeatedly registered subject. By this a right of the faculty to enable examination by the committee in other cases, if implied by the internal regulations (Article 19, Paragraph 2), is not affected.
The credit requirements and further information on the examinations can be found in the announcements on the website of biochemical institute that provides the classes in the given academic year.
Poslední úprava: Pláteník Jan, MUDr., Ph.D. (12.01.2021)
Literatura
H.S. Stoker: General, Organic and Biological Chemistry. Houghton Mifflin Co., Boston, (latest edition).
P.C. Champe, R.A. Harvey, and D.R. Ferrier: Lippincott?s Illustrated Reviews: Biochemistry. Lippincott Williams & Wilkins (latest edition)
R.K. Murray et al.: Harper?s Illustrated Biochemistry. McGraw-Hill, Inc., USA (latest edition)
B. Alberts et al.: Essential Cell Biology. Garland Science Publishing, New York (latest edition)
J.M. Gerg, J.L. Tymoczko, L. Stryer: Biochemistry. Freeman, New York (latest edition).
Poslední úprava: Kohútová Vladislava, Ing. (07.01.2019)
Požadavky ke zkoušce - angličtina
LIST OF QUESTIONS FROM MEDICAL CHEMISTRY AND BIOCHEMISTRY FOR THE EXAM
I.Fundamentals of physical, inorganic, and organic chemistry
1.Chemical bonding (covalent and coordinate covalent), ionic bonding; weak interactions and hydrophobic exclusion, and their properties.
2.Water and its physical and chemical properties, importance in the organism.
3.Disperse systems – classification, examples from biochemistry. Solubility of substances.
4.Diffusion, osmosis, osmotic and oncotic pressure, dialysis, examples from biochemistry.
5.Energy of chemical reactions, Gibbs energy and entropy, application in metabolic processes.
6.Chemical equilibrium, Guldberg-Waage law. Kinetics and energetics of reversible reactions, application in enzymology.
7.Basic methods for the separation of macromolecules (electrophoresis, chromatography, mass spectrometry) and their use in clinical practice.
8.Spectrophotometry, principle and applications in clinical biochemistry.
9.Electrolytic dissociation, dissociation constant, strong and weak electrolytes, examples from biochemistry.
10.Brønsted theory of acids and bases, equilibrium in protolytic reactions, examples from biochemistry. Ion product of water, pH and its significance in medicine.
11.Ampholytes- properties and examples from biochemistry.
12.Buffers- pH calculation and significance in the human body.
13.Oxidation and reduction, equilibrium in redox reactions, oxidation-reduction potential, dependence on reactant concentration (Nernst equation). Examples from biochemistry.
14. Precipitation reactions, solubility product, complex formation, coordination compounds, examples. Importance in biochemistry and medicine.
15. Chemical properties of macrobiogenic elements (C, H, O, N, P, Na, K, Ca, Mg, S, Cl).
16. Oxygen and its inorganic compounds, reactivity, properties.
17.Examples of mechanisms of action of toxicologically important elements and inorganic compounds (e.g., heavy metals, chlorine, CO, cyanides).
18.Biological and metabolic significance of microbiogenic and trace elements (Fe, Zn, Cu, I, Se, Co, Mn).
19.Structure of organic compounds, types of isomerism, examples in biochemistry.
20.Amines and nitro derivatives of hydrocarbons, examples and significance in biochemistry.
21.Halogen derivatives and sulfur derivatives (thiols, sulfonic acids) of hydrocarbons. Examples and significance in biochemistry and medicine.
22.Alcohols, phenols, aldehydes, and ketones- role in metabolism.
23.Carboxylic acids and their functional and substitution derivatives, role in biochemistry.
24.Examples of biochemically important heterocycles (e.g., pyrimidine, pyridine, pyrrole, purine, furan, pyran, indole, thiophene), importance, properties, biochemical significance of heteroatoms.
25.Structural characteristics of amino acids, classification, reactions, significance.
26.Peptides, peptide bond, examples of biologically important peptides.
27.Proteins, primary, secondary, tertiary, and quaternary structure. Suprasecondary structures, protein domains. Protein folding disorders. Properties and functions of proteins.
34.Phospholipids and sphingolipids – classification, structure, properties, biological significance.
35.Sterols, bile acids and steroid hormones, structure, function and significance in the body.
II. Fundamentals of metabolism
1. Structure of enzymes, protein and non-protein components. Monomeric enzymes and enzymes composed of multiple subunits. Isoenzymes. Classification and nomenclature of enzymes.
2. Cofactors, coenzymes, and prosthetic groups. Enzyme activators and coactivators. Examples, biological significance.
3. Regulation of enzyme activity, significance for the control of metabolic processes, examples. Physicochemical factors, allosteric effects. Principles of enzyme activity measurement, significance in medicine.
4. Mechanism and energetics of enzyme catalysis. Kinetics of enzymatic reactions.
5. Enzyme inhibition: competitive, non-competitive, covalent, allosteric. Use of enzyme
inhibitors in medicine.
6. Respiratory chain. Oxidative phosphorylation. Shuttles for electron transport across mitochondrial membranes.
7. High-energy (“macroergic”) compounds, substrate level phosphorylation, coupling of exergonic and endergonic reactions.
8. Citric acid cycle, amphibolic character, course, and regulation.
9. General mechanisms of amino acid conversion, deamination, transamination, decarboxylation.
10. Formation and detoxification of ammonia, urea cycle, its regulation and disorders. Nitrogen balance.
11. Metabolism of pyruvate and oxaloacetate group amino acids, involvement of these amino acids in metabolic processes.
12. Metabolism of the carbon skeleton of amino acids of the 2-oxoglutarate group, amino acids with a branched chain, involvement of these amino acids in metabolic processes.
13. Catabolism of phenylalanine and tyrosine, disorders.
14. Metabolism of sulfur amino acids. Metabolism of tetrahydrofolate and S-adenosylmethionine.
15. Biogenic amines: biosynthesis, biodegradation, common characteristics, examples.
16. Conversion of amino acids into specialized products: creatine, carnitine, taurine, melanins and their significance.
17. Glycolysis, energy balance, utilization of glycolysis by individual organs of the body in various physiological situations, regulation, pyruvate oxidation, pyruvate dehydrogenase complex.
18. Gluconeogenesis, significance, regulation.
19. Glycogen synthesis and degradation, significance, regulation, disorders.
4.Regulation of heme biosynthesis, differences between hepatocytes and erythroid cells, iron metabolism.
5.Mechanism of action of hormones regulating water and mineral metabolism.
6.Hormonal regulation of energy metabolism.
7.Biochemical processes in the digestion of carbohydrates, lipids, and proteins.
8.Biochemical functions of hepatocytes and the liver, biochemical markers of hepatocellular injury and liver function.
9.Biotransformation of endogenous and exogenous substances, types of biotransformation processes, toxic and carcinogenic substances in the environment.
10.Buffer systems of the body, function and significance for acid-base balance.
11.Erythrocyte metabolism.
12.Significance of blood plasma proteins (albumin, Ig, acute phase proteins, transport proteins).
13.Hemocoagulation, coagulation cascade, initiation, amplification and propagation, tenase and prothrombinase complexes. Role of thrombocytes and protein C.
14. Fibrin, fibrinolysis. Mechanism of action of anticoagulants.
15. Urine – physiological and pathological components.
28.Thyroid hormones and their function in regulatory processes.
29.Humoral immunity. Structure and function of immunoglobulins. Complement. CRP.
30.Classes of immunoglobulins, properties and functions. Molecular basis of immunoglobulin diversity in primary and secondary antibody responses, somatic recombination, isotype switching.
31.Basic immunochemical methods. Immunoturbidimetry, immunoanalytical methods with labeled antibodies or antigens. Monoclonal antibodies – production and use.
32.Biochemical aspects of cellular immunity- antigen presentation, recognition of ligands by PRRs, respiratory burst, degradation of material in the phagosome, processing of antigen for presentation, formation of peroxynitrite.
33.Biochemical significance of fat-soluble vitamins.
34.Biochemical significance of water-soluble vitamins, cofactors derived from these vitamins.
35.Structure, composition, and properties of cell membranes.
36.Transport of substances across membranes.
37.Cytoskeleton.
38.Compartmentalization of biochemical processes at the subcellular level.
39.Biochemical functions of the kidneys. Principles of biochemical diagnosis of renal damage.
IV. Fundamentals of cellular and molecular Biology
1. Structure and function of DNA.
2. Structure, types, and function of RNA.
3. Organization of prokaryotic, eukaryotic, and mitochondrial genomes.
4. Principles of DNA sequencing (Sanger, NGS).
5. Replication of eukaryotic DNA, the replication apparatus, and its regulation.
6. DNA repair – BER, NER, MMR, direct repair of modified bases.
7. Repair of double-strand DNA breaks – HR, NHEJ.
8. Transcription of prokaryotic and eukaryotic genomic DNA. Transcription factors, DNA-protein interactions.
9. Structure of mRNA, post-transcriptional modifications (cap, poly A, splicing).
10. RNA interference, types and functions of non-coding RNAs.
11. Mechanisms regulating gene expression.
12. Genetic code and its properties.
13. Eukaryotic and prokaryotic translation. Regulation of translation.
14. Sorting, transport, and post-translational modifications of proteins. Biosynthesis of glycoproteins and their significance.
15. Vesicular transport. Endocytosis and exocytosis.
16. Restriction enzymes and other tools of genetic engineering, construction of recombinant DNA molecules and proteins. DNA cloning.
17. Methods of cell fractionation, electrophoresis of nucleic acids and proteins.
18. PCR, RT-PCR, and qPCR- principles and application in clinical diagnostics.
19. Nature of gene mutations, hereditary and acquired mutations, polymorphisms, mini- and microsatellite sequences and their diagnostic use.
20. Principles, mechanisms, and significance of intercellular communication and intracellular signal transduction cascades. Signal amplification and cross-talk of signaling pathways.
21. Classification of membrane receptors, their ligands, biological significance.
22. Classification of intracellular receptors, their ligands, biological significance.
23. Biochemical principles of signaling pathway-targeted therapy. Examples.
24. G-proteins, structure, functions.
25. Types and role of second messengers in signal transduction.
26. Mechanisms and significance of reversible phosphorylation in signal transduction.
27. Signaling pathways stimulated by growth factors (MAPK, PKB/AKT) and cytokines (JAK - STAT).
28. Proteolysis-dependent signaling pathways, examples. HIF-mediated signaling in response to hypoxia.
29. NO-mediated signaling, medical significance.
30. Proto-oncogenes.
31. Tumor suppressor genes.
32. Cell cycle, role of cyclin and CDK (cyclin-dependent kinase) complexes.
33. Lysosomal and ubiquitin-proteasome-mediated protein degradation.
34. Biochemistry of apoptosis, examples of pro- and anti-apoptotic proteins. Caspases. The role of mitochondria in cell death.
35. Epigenetic mechanisms- histone modifications, DNA methylation, biological significance.
36. Molecular mechanisms of cell senescence: the role of telomeres, cell cycle regulation, and the connection to organism aging.
Poslední úprava: Stollinová Šromová Lucie, Mgr., Ph.D. (23.02.2026)
Sylabus - angličtina
Introduction to physical, inorganic, and organic chemistry - principles of chemical processes, structures and characteristics of substances that are present in the human body under physiological and pathological conditions and general principles of their metabolism, basics of the metabolism of xenobiotics.
Overview of metabolic pathways - turnover of body fuels, interplay of their metabolic pathways during production, storage, and interconversion of energy and synthesis and degradation of structural and informational molecules.
Molecular biology principles of body functions - dynamics of information molecules, intracellular, intercellular, and systemic mechanisms of metabolic regulations, illustrative examples from the biochemistry of organ systems and molecular defects leading to human diseases.
Concepts of bioanalytical approaches - methods for the detection of biochemical and molecular biology markers in human medicine
Poslední úprava: Kolářová Jana (04.10.2019)
Rozpis výuky -
Rozpis blokový
Týden bloku
Den
Od - Do
Typ výuky
Téma
Učitel
Soubory
Poznámka
Hodnocení
1
Úterý
14:15 - 17:15
praktická cvičení
Laboratory rules and principles of work safety. Photometry
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Laboratory rules and principles of work safety. Photometry
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Laboratory rules and principles of work safety. Photometry
Calculations in medicinal chemistry and biochemistry
Mgr. Lucie Stollinová Šromová, Ph.D.
Lecture hall 1.26 u nemocnice 4
Úterý
14:15 - 17:15
praktická cvičení
Acids, bases, pH (theory and calculations), buffers (theory and calculations)
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Acids, bases, pH (theory and calculations), buffers (theory and calculations)
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Acids, bases, pH (theory and calculations), buffers (theory and calculations)
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Pátek
14:15 - 15:45
seminář
Calculations in medicinal chemistry and biochemistry
Mgr. Lucie Stollinová Šromová, Ph.D.
Lecture hall 097 – Cori, U nemocnice
4
Úterý
14:15 - 17:15
praktická cvičení
Properties and reactions of biologically and toxicologically important elements and their compounds. Solubility, complex compounds, repetition- biogenic and toxic elements, Pb, Hg, As.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Properties and reactions of biologically and toxicologically important elements and their compounds. Solubility, complex compounds, repetition- biogenic and toxic elements, Pb, Hg, As.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Properties and reactions of biologically and toxicologically important elements and their compounds. Solubility, complex compounds, repetition- biogenic and toxic elements, Pb, Hg, As.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
5
Pondělí
14:15 - 15:45
seminář
Organic Chemistry - repetition.
MUDr. Eva Balážiová, Ph.D.
Lecture hall 1.26, U nemocnice 4
Úterý
14:15 - 17:15
praktická cvičení
Reactions of biochemically important organic compounds. Classification and basic nomenclature of organic compounds.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Reactions of biochemically important organic compounds. Classification and basic nomenclature of organic compounds.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Reactions of biochemically important organic compounds. Classification and basic nomenclature of organic compounds.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Pátek
14:15 - 15:45
seminář
Organic Chemistry - repetition.
MUDr. Eva Balážiová, Ph.D.
Lecture hall 097 – Cori, U nemocnice
6
Pondělí
14:15 - 15:45
seminář
Proteins - properties, functions.
Mgr. Helena Kupcová Skalníková, Ph.D.
Lecture hall 1.26, U nemocnice 4
Úterý
14:15 - 17:15
praktická cvičení
Control test.
Amino acids and peptides.
MUDr. Martin Vejražka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Control test.
Amino acids and peptides.
MUDr. Martin Vejražka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Control test.
Amino acids and peptides.
MUDr. Martin Vejražka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Pátek
14:15 - 15:45
seminář
Proteins - properties, functions.
Mgr. Helena Kupcová Skalníková, Ph.D.
Lecture hall 097 – Cori, U nemocnice
7
Pondělí
14:15 - 15:45
seminář
Enzymes - definition and characterization, importance of enzymes and isoenzymes in medicine.
Mgr. Lucie Stollinová Šromová, Ph.D.
Lecture hall 1.26, U nemocnice 4
Úterý
14:15 - 17:15
praktická cvičení
Structure and properties of proteins, selected methods for studying proteins
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Structure and properties of proteins, selected methods for studying proteins
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Structure and properties of proteins, selected methods for studying proteins
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Pátek
14:15 - 15:45
seminář
Enzymes - definition and characterization, importance of enzymes and isoenzymes in medicine.
Mgr. Lucie Stollinová Šromová, Ph.D.
Lecture hall 097 – Cori, U nemocnice
8
Úterý
14:15 - 17:15
praktická cvičení
Enzyme, enzyme activity and its modulation (effectors and inhibitors).
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Enzyme, enzyme activity and its modulation (effectors and inhibitors).
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Enzyme, enzyme activity and its modulation (effectors and inhibitors).
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
9
Úterý
14:15 - 17:15
praktická cvičení
Biological oxidation
RNDr. Jakub Červenka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Biological oxidation
RNDr. Jakub Červenka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Biological oxidation
RNDr. Jakub Červenka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
10
Pondělí
14:15 - 15:45
seminář
Overview of carbohydrates metabolic pathways
Mgr. Helena Kupcová Skalníková, Ph.D.
Lecture hall 1.26, U nemocnice 4
Úterý
14:15 - 17:15
praktická cvičení
Control test.
Glycosamino glycans. Dietary fiber.
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Control test.
Glycosamino glycans. Dietary fiber.
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Control test.
Glycosamino glycans. Dietary fiber.
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Pátek
14:15 - 15:45
seminář
Overview of carbohydrates metabolic pathways
Mgr. Helena Kupcová Skalníková, Ph.D.
Lecture hall 097 – Cori, U nemocnice
11
Úterý
14:15 - 17:15
praktická cvičení
Bile acids, lipid digestion.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Bile acids, lipid digestion.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Bile acids, lipid digestion.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
12
Pondělí
14:15 - 15:45
seminář
Lipids -overview
RNDr. Ivana Matrasová, Ph.D.
Lecture hall 1.26, U nemocnice 4
Úterý
14:15 - 17:15
praktická cvičení
Cholesterol, triacylglycerols, lipoproteins .
RNDr. Jakub Červenka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Cholesterol, triacylglycerols, lipoproteins .
RNDr. Jakub Červenka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Cholesterol, triacylglycerols, lipoproteins .
RNDr. Jakub Červenka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Pátek
14:15 - 15:45
seminář
Lipids -overview
RNDr. Ivana Matrasová, Ph.D.
Lecture hall 097 – Cori, U nemocnice
13
Úterý
14:15 - 17:15
praktická cvičení
Transamination, deamination, urea cycle. Defects of the urosynthetic cycle - hyperammonemia.Selected parameters of nutritional status - determination of albumin concentration. Protein electrophoresis
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Transamination, deamination, urea cycle. Defects of the urosynthetic cycle - hyperammonemia.Selected parameters of nutritional status - determination of albumin concentration. Protein electrophoresis
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Transamination, deamination, urea cycle. Defects of the urosynthetic cycle - hyperammonemia.Selected parameters of nutritional status - determination of albumin concentration. Protein electrophoresis
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
14
Pondělí
14:15 - 15:45
seminář
Amino acid(AA) metabolism and selected disorders of AA metabolism
Mgr. Magdalena Houdová Megová, Ph.D.
Lecture hall 1.26, U nemocnice 4
Úterý
14:15 - 17:15
praktická cvičení
Control test.
Tetrapyrroles
MUDr. Eva Balážiová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Středa
14:15 - 17:15
praktická cvičení
Control test.
Tetrapyrroles
MUDr. Eva Balážiová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Čtvrtek
14:15 - 17:15
praktická cvičení
Control test.
Tetrapyrroles
MUDr. Eva Balážiová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Pátek
14:15 - 15:45
seminář
Amino acid(AA) metabolism and selected disorders of AA metabolism
Mgr. Magdalena Houdová Megová, Ph.D.
Lecture hall 097 – Cori, U nemocnice 5
Rozpis blokový
Týden bloku
Den
Od - Do
Typ výuky
Téma
Učitel
Soubory
Poznámka
Hodnocení
1
Úterý
14:15 - 17:15
praktická cvičení
Determination of uric acid
Mgr. Soňa Gyönyörová
Středa
14:15 - 17:15
praktická cvičení
Determination of uric acid
Mgr. Soňa Gyönyörová
Čtvrtek
14:15 - 17:15
praktická cvičení
Determination of uric acid
Mgr. Soňa Gyönyörová
2
Úterý
14:15 - 17:15
praktická cvičení
Calculations in molecular biology
RNDr. Jan Stejskal
Středa
14:15 - 17:15
praktická cvičení
Calculations in molecular biology
RNDr. Jan Stejskal
Čtvrtek
14:15 - 17:15
praktická cvičení
Calculations in molecular biology
RNDr. Jan Stejskal
3
Pondělí
14:15 - 15:45
seminář
Summarizing seminar- structure and function of nucleic acids, RNA types, genome organization, chromatin structure.
Mgr. Magdalena Houdová Megová, Ph.D.
Úterý
14:15 - 17:15
praktická cvičení
Isolation of genetic material from buccal mucosal swabs. Measurement of isolated DNA concentration.
Středa
14:15 - 17:15
praktická cvičení
Isolation of genetic material from buccal mucosal swabs. Measurement of isolated DNA concentration.
Čtvrtek
14:15 - 17:15
praktická cvičení
Isolation of genetic material from buccal mucosal swabs. Measurement of isolated DNA concentration.
Pátek
14:15 - 15:45
seminář
Summarizing seminar- structure and function of nucleic acids, RNA types, genome organization, chromatin structure.
Mgr. Magdalena Houdová Megová, Ph.D.
4
Pondělí
14:15 - 15:45
seminář
Next Generation sequencing
MUDr. František Sedlák, Ph.D.
Úterý
14:15 - 17:15
praktická cvičení
Electrophoresis of isolated DNA. PCR amplification of the stretch including exon 13 of the DPYD gene.
Středa
14:15 - 17:15
praktická cvičení
Electrophoresis of isolated DNA. PCR amplification of the stretch including exon 13 of the DPYD gene.
Čtvrtek
14:15 - 17:15
praktická cvičení
Electrophoresis of isolated DNA. PCR amplification of the stretch including exon 13 of the DPYD gene.
Pátek
14:15 - 15:45
seminář
Next Generation sequencing
MUDr. František Sedlák, Ph.D.
5
Pondělí
14:15 - 15:45
seminář
Regulation of gene expression
RNDr. Jakub Červenka, Ph.D.
Úterý
14:15 - 17:15
praktická cvičení
PCR electrophoresis and sequence preparation, evaluation in FINCH
Středa
14:15 - 17:15
praktická cvičení
PCR electrophoresis and sequence preparation, evaluation in FINCH
Čtvrtek
14:15 - 17:15
praktická cvičení
PCR electrophoresis and sequence preparation, evaluation in FINCH
Pátek
14:15 - 15:45
seminář
Regulation of gene expression
RNDr. Jakub Červenka, Ph.D.
6
Úterý
14:15 - 17:15
praktická cvičení
MB block evaluation
Středa
14:15 - 17:15
praktická cvičení
MB block evaluation
Čtvrtek
14:15 - 17:15
praktická cvičení
MB block evaluation
7
Pondělí
14:15 - 15:45
seminář
Transmembrane transport (proton pump, Wilson's disease, cystic fibrosis)
Pátek
14:15 - 15:45
seminář
Transmembrane transport (proton pump, Wilson's disease, cystic fibrosis)
8
Pondělí
14:15 - 17:15
praktická cvičení
Analysis of urine and urinary sediment in diagnostics
14:15 - 15:45
seminář
Protein folding, ubiquitin proteasome system (misfolding diseases, chaperones, defects in the ubiquitin-proteasome system- VHL, parkin, HPV induced)
doc. MUDr. Petr Bušek, Ph.D.
Úterý
14:15 - 17:15
praktická cvičení
Analysis of urine and urinary sediment in diagnostics
Středa
14:15 - 17:15
praktická cvičení
Analysis of urine and urinary sediment in diagnostics
Čtvrtek
14:15 - 17:15
praktická cvičení
Analysis of urine and urinary sediment in diagnostics
Pátek
14:15 - 15:45
seminář
Protein folding, ubiquitin proteasome system (misfolding diseases, chaperones, defects in the ubiquitin-proteasome system- VHL, parkin, HPV induced)
doc. MUDr. Petr Bušek, Ph.D.
9
Pondělí
14:15 - 15:45
seminář
Preanalytical aspects of biochemical examination
Úterý
14:15 - 17:15
praktická cvičení
Acid-base balance
MUDr. Eva Balážiová, Ph.D.
Středa
14:15 - 17:15
praktická cvičení
Acid-base balance
MUDr. Eva Balážiová, Ph.D.
Čtvrtek
14:15 - 17:15
praktická cvičení
Acid-base balance
MUDr. Eva Balážiová, Ph.D.
Pátek
14:15 - 15:45
seminář
Preanalytical aspects of biochemical examination
10
Pondělí
14:15 - 15:45
seminář
Membrane receptors and their signalling. Cytokines and growth factors (erythropoietin, insulin, VEGF, EGF). Congenital and acquired defects in signalling molecules and cancer
Úterý
14:15 - 17:15
praktická cvičení
Control test Kidney biochemistry /clearance
RNDr. Ivana Matrasová, Ph.D.
Středa
14:15 - 17:15
praktická cvičení
Control test Kidney biochemistry /clearance
RNDr. Ivana Matrasová, Ph.D.
Čtvrtek
14:15 - 17:15
praktická cvičení
Control test Kidney biochemistry /clearance
RNDr. Ivana Matrasová, Ph.D.
Pátek
14:15 - 15:45
seminář
Membrane receptors and their signalling. Cytokines and growth factors (erythropoietin, insulin, VEGF, EGF). Congenital and acquired defects in signalling molecules and cancer
11
Pondělí
14:15 - 15:45
seminář
Membrane receptors and their signalling. Cytokines and growth factors (erythropoietin, insulin, VEGF, EGF). Congenital and acquired defects in signalling molecules and cancer
Úterý
14:15 - 17:15
praktická cvičení
Examination in patients with diabetes mellitus
Středa
14:15 - 17:15
praktická cvičení
Examination in patients with diabetes mellitus
Čtvrtek
14:15 - 17:15
praktická cvičení
Examination in patients with diabetes mellitus
Pátek
14:15 - 15:45
seminář
Membrane receptors and their signalling. Cytokines and growth factors (erythropoietin, insulin, VEGF, EGF). Congenital and acquired defects in signalling molecules and cancer
12
Pondělí
14:15 - 15:45
seminář
Summary seminar - signalling
Úterý
14:15 - 17:15
praktická cvičení
Xenobiochemistry and toxicology /Ethanol in breath. Detection of drugs in urine
Středa
14:15 - 17:15
praktická cvičení
Xenobiochemistry and toxicology /Ethanol in breath. Detection of drugs in urine
Čtvrtek
14:15 - 17:15
praktická cvičení
Xenobiochemistry and toxicology /Ethanol in breath. Detection of drugs in urine
Pátek
14:15 - 15:45
seminář
Summary seminar - signalling
13
Pondělí
14:15 - 15:45
seminář
Summary of energy metabolism control
Úterý
14:15 - 17:15
praktická cvičení
Biochemistry of muscle tissue/ Determination of selected markers of muscle damage
Středa
14:15 - 17:15
praktická cvičení
Biochemistry of muscle tissue/ Determination of selected markers of muscle damage
Čtvrtek
14:15 - 17:15
praktická cvičení
Biochemistry of muscle tissue/ Determination of selected markers of muscle damage
Pátek
14:15 - 15:45
seminář
Summary of energy metabolism control
14
Pondělí
14:15 - 15:45
seminář
Overview of immunological methods- Monoclonal antibodies- preparation and use. Basic immunochemical methods. Immunoturbidimetry, ELISA, RIA.
Úterý
14:15 - 17:15
praktická cvičení
Immunological methods in clinical practice
Control test
Středa
14:15 - 17:15
praktická cvičení
Immunological methods in clinical practice
Control test
Čtvrtek
14:15 - 17:15
praktická cvičení
Immunological methods in clinical practice
Control test
Pátek
14:15 - 15:45
seminář
Overview of immunological methods- Monoclonal antibodies- preparation and use. Basic immunochemical methods. Immunoturbidimetry, ELISA, RIA.
Rozpis datumový
Den
Datum
Popis
Učitel
Soubory
Poznámka
Hodnocení
Pondělí
29.09.2025
Introduction to medicinal chemistry and biochemistry. Water and aqueous dispersion systems.
prof. MUDr. Aleksi Šedo, DrSc.
Čtvrtek
02.10.2025
Acidobasis. pH and buffers, reactions.
Mgr. Lucie Stollinová Šromová, Ph.D.
Pondělí
06.10.2025
Chemical reactions.
doc. MUDr. Petr Bušek, Ph.D.
Čtvrtek
09.10.2025
Biogenic elements and medically important inorganic compounds.
Mgr. Lucie Stollinová Šromová, Ph.D.
Pondělí
13.10.2025
Medically important organic compounds.
MUDr. František Sedlák, Ph.D.
Čtvrtek
16.10.2025
Saccharides - overview.
RNDr. Ivana Matrasová, Ph.D.
Pondělí
20.10.2025
Medically important heterocyclic compounds.
Mgr. Jan Ševčík, Ph.D.
Čtvrtek
23.10.2025
Amino acids and peptides.
doc. MUDr. Petr Bušek, Ph.D.
Pondělí
27.10.2025
Structure of proteins and their function in the organism.
doc. MUDr. Petr Bušek, Ph.D.
Čtvrtek
30.10.2025
Lipids - an overview.
MUDr. Martin Vejražka, Ph.D.
Pondělí
03.11.2025
Enzymes 1
prof. MUDr. Aleksi Šedo, DrSc.
Čtvrtek
06.11.2025
Enzymes 2
prof. MUDr. Aleksi Šedo, DrSc.
Pondělí
10.11.2025
Vitamins
doc. MUDr. Jaromír Křemen, CSc.
Čtvrtek
13.11.2025
Introduction to metabolism (biochemistry and energy)
doc. MUDr. Jaromír Křemen, CSc.
Čtvrtek
20.11.2025
Makroergic compounds. Respiratory chain and oxidative phosphorylation.
Metabolism of glycerophospholipids and sphingolipids. Eikosanoids.
MUDr. Martin Vejražka, Ph.D.
Pondělí
05.01.2026
Protein digestion, transamination, ureosynthetic cycle.
doc. MUDr. Petr Bušek, Ph.D.
Čtvrtek
08.01.2026
Metabolism of the carbon skeleton of amino acids.
doc. MUDr. Petr Bušek, Ph.D.
Pondělí
12.01.2026
Metabolism of aromatic amino acids and formation of specialized products.
doc. MUDr. Petr Bušek, Ph.D.
Čtvrtek
15.01.2026
Metabolism of tetrapyrroles.
MUDr. Ivan Melezínek, CSc., mimořádný profesor Univerzity Karlovy
Pondělí
19.01.2026
Purine and pyrimidine metabolism.
doc. MUDr. Jaromír Křemen, CSc.
Rozpis datumový
Den
Datum
Popis
Učitel
Soubory
Poznámka
Hodnocení
Středa
25.02.2026
Introduction to Molecular Biology (Biochemistry Information)
Mgr. Martin Sztacho, Ph.D.
Čtvrtek
26.02.2026
Structure and function of nucleic acids, types of RNA molecules
Mgr. Martin Sztacho, Ph.D.
Středa
04.03.2026
Organization of prokaryotic, eukaryotic and mitochondrial genomes; structure of chromatin
Mgr. Martin Sztacho, Ph.D.
Čtvrtek
05.03.2026
DNA replication in prokaryotic and eukaryotic cells.
Mgr. Martin Sztacho, Ph.D.
Středa
11.03.2026
Mutations and their origin and effects. Mutagens and carcinogens.
Mgr. Helena Kupcová Skalníková, Ph.D.
Čtvrtek
12.03.2026
DNA mutation repair
Mgr. Helena Kupcová Skalníková, Ph.D.
Středa
18.03.2026
RNA biosynthesis - DNA transcription, modifications of primary transcript of prokaryotes and eukaryotes, alternative splicing. RNA transport and degradation in eukaryotes.
MUDr. František Sedlák, Ph.D.
Čtvrtek
19.03.2026
Regulation of gene expression.
MUDr. František Sedlák, Ph.D.
Středa
25.03.2026
Translation , inhibitors of prokaryotic and eukaryotic translation. Protein folding, chaperones. Post-translational modification of proteins 1.
MUDr. František Sedlák, Ph.D.
Čtvrtek
26.03.2026
Post-translational covalent modification of proteins 2. Sorting and transport of proteins into individual organelles, secretory proteins.
MUDr. František Sedlák, Ph.D.
Středa
01.04.2026
Structure and function of cell membranes, cell junctions.
doc. MUDr. Petr Bušek, Ph.D.
Středa
08.04.2026
Virus structure, replication and assembly.
doc. MUDr. Jaromír Křemen, CSc.
Středa
08.04.2026
Compartmentation of the cell, structure and function of cell organelles, vesicular transport.
MUDr. Martin Vejražka, Ph.D.
Čtvrtek
09.04.2026
Mechanisms of signal transduction 1
prof. MUDr. Aleksi Šedo, DrSc.
Středa
15.04.2026
Mechanisms of signal transduction 2
prof. MUDr. Aleksi Šedo, DrSc.
Čtvrtek
16.04.2026
Mechanisms of signal transduction 3
prof. MUDr. Aleksi Šedo, DrSc.
Středa
22.04.2026
Biochemistry of peptide and protein hormones and thyroid hormones.
prof. MUDr. Aleksi Šedo, DrSc.
Čtvrtek
23.04.2026
Hormones and signaling pathways affecting energy metabolism.
prof. MUDr. Aleksi Šedo, DrSc.
Středa
29.04.2026
Apoptosis
MUDr. Ivan Melezínek, CSc., mimořádný profesor Univerzity Karlovy
Čtvrtek
30.04.2026
Cell cycle
MUDr. Ivan Melezínek, CSc., mimořádný profesor Univerzity Karlovy
Středa
06.05.2026
Liver biochemistry
doc. MUDr. Jaromír Křemen, CSc.
Čtvrtek
07.05.2026
Xenobiochemistry
Mgr. Magdalena Houdová Megová, Ph.D.
Čtvrtek
14.05.2026
Biochemistry of blood and hemocoagulation.
doc. MUDr. Petr Bušek, Ph.D.
Středa
20.05.2026
Muscle biochemistry and muscle contraction
MUDr. Eva Balážiová, Ph.D.
Čtvrtek
21.05.2026
Humoral immunity
doc. MUDr. Petr Bušek, Ph.D.
Středa
27.05.2026
Cellular immunity
doc. MUDr. Petr Bušek, Ph.D.
Čtvrtek
28.05.2026
Biochemistry of neuronal tissue and senses.
MUDr. Eva Balážiová, Ph.D.
Středa
03.06.2026
Biochemistry of connective tissue and skin.
MUDr. Ivan Melezínek, CSc., mimořádný profesor Univerzity Karlovy
Schedule block
Week of the block
Day
From - To
Education type
Theme
Teacher
Files
Note
Hodnocení
1
Tuesday
14:15 - 17:15
practical classes
Laboratory rules and principles of work safety. Photometry
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Laboratory rules and principles of work safety. Photometry
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Laboratory rules and principles of work safety. Photometry
Calculations in medicinal chemistry and biochemistry
Mgr. Lucie Stollinová Šromová, Ph.D.
Lecture hall 1.26 u nemocnice 4
Tuesday
14:15 - 17:15
practical classes
Acids, bases, pH (theory and calculations), buffers (theory and calculations)
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Acids, bases, pH (theory and calculations), buffers (theory and calculations)
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Acids, bases, pH (theory and calculations), buffers (theory and calculations)
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Friday
14:15 - 15:45
seminar
Calculations in medicinal chemistry and biochemistry
Mgr. Lucie Stollinová Šromová, Ph.D.
Lecture hall 097 – Cori, U nemocnice
4
Tuesday
14:15 - 17:15
practical classes
Properties and reactions of biologically and toxicologically important elements and their compounds. Solubility, complex compounds, repetition- biogenic and toxic elements, Pb, Hg, As.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Properties and reactions of biologically and toxicologically important elements and their compounds. Solubility, complex compounds, repetition- biogenic and toxic elements, Pb, Hg, As.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Properties and reactions of biologically and toxicologically important elements and their compounds. Solubility, complex compounds, repetition- biogenic and toxic elements, Pb, Hg, As.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
5
Monday
14:15 - 15:45
seminar
Organic Chemistry - repetition.
MUDr. Eva Balážiová, Ph.D.
Lecture hall 1.26, U nemocnice 4
Tuesday
14:15 - 17:15
practical classes
Reactions of biochemically important organic compounds. Classification and basic nomenclature of organic compounds.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Reactions of biochemically important organic compounds. Classification and basic nomenclature of organic compounds.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Reactions of biochemically important organic compounds. Classification and basic nomenclature of organic compounds.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Friday
14:15 - 15:45
seminar
Organic Chemistry - repetition.
MUDr. Eva Balážiová, Ph.D.
Lecture hall 097 – Cori, U nemocnice
6
Monday
14:15 - 15:45
seminar
Proteins - properties, functions.
Mgr. Helena Kupcová Skalníková, Ph.D.
Lecture hall 1.26, U nemocnice 4
Tuesday
14:15 - 17:15
practical classes
Control test.
Amino acids and peptides.
MUDr. Martin Vejražka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Control test.
Amino acids and peptides.
MUDr. Martin Vejražka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Control test.
Amino acids and peptides.
MUDr. Martin Vejražka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Friday
14:15 - 15:45
seminar
Proteins - properties, functions.
Mgr. Helena Kupcová Skalníková, Ph.D.
Lecture hall 097 – Cori, U nemocnice
7
Monday
14:15 - 15:45
seminar
Enzymes - definition and characterization, importance of enzymes and isoenzymes in medicine.
Mgr. Lucie Stollinová Šromová, Ph.D.
Lecture hall 1.26, U nemocnice 4
Tuesday
14:15 - 17:15
practical classes
Structure and properties of proteins, selected methods for studying proteins
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Structure and properties of proteins, selected methods for studying proteins
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Structure and properties of proteins, selected methods for studying proteins
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Friday
14:15 - 15:45
seminar
Enzymes - definition and characterization, importance of enzymes and isoenzymes in medicine.
Mgr. Lucie Stollinová Šromová, Ph.D.
Lecture hall 097 – Cori, U nemocnice
8
Tuesday
14:15 - 17:15
practical classes
Enzyme, enzyme activity and its modulation (effectors and inhibitors).
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Enzyme, enzyme activity and its modulation (effectors and inhibitors).
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Enzyme, enzyme activity and its modulation (effectors and inhibitors).
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
9
Tuesday
14:15 - 17:15
practical classes
Biological oxidation
RNDr. Jakub Červenka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Biological oxidation
RNDr. Jakub Červenka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Biological oxidation
RNDr. Jakub Červenka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
10
Monday
14:15 - 15:45
seminar
Overview of carbohydrates metabolic pathways
Mgr. Helena Kupcová Skalníková, Ph.D.
Lecture hall 1.26, U nemocnice 4
Tuesday
14:15 - 17:15
practical classes
Control test.
Glycosamino glycans. Dietary fiber.
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Control test.
Glycosamino glycans. Dietary fiber.
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Control test.
Glycosamino glycans. Dietary fiber.
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Friday
14:15 - 15:45
seminar
Overview of carbohydrates metabolic pathways
Mgr. Helena Kupcová Skalníková, Ph.D.
Lecture hall 097 – Cori, U nemocnice
11
Tuesday
14:15 - 17:15
practical classes
Bile acids, lipid digestion.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Bile acids, lipid digestion.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Bile acids, lipid digestion.
RNDr. Jan Stejskal
laboratory of practical lessons, U Nemocnice 5
12
Monday
14:15 - 15:45
seminar
Lipids -overview
RNDr. Ivana Matrasová, Ph.D.
Lecture hall 1.26, U nemocnice 4
Tuesday
14:15 - 17:15
practical classes
Cholesterol, triacylglycerols, lipoproteins .
RNDr. Jakub Červenka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Cholesterol, triacylglycerols, lipoproteins .
RNDr. Jakub Červenka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Cholesterol, triacylglycerols, lipoproteins .
RNDr. Jakub Červenka, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Friday
14:15 - 15:45
seminar
Lipids -overview
RNDr. Ivana Matrasová, Ph.D.
Lecture hall 097 – Cori, U nemocnice
13
Tuesday
14:15 - 17:15
practical classes
Transamination, deamination, urea cycle. Defects of the urosynthetic cycle - hyperammonemia.Selected parameters of nutritional status - determination of albumin concentration. Protein electrophoresis
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Transamination, deamination, urea cycle. Defects of the urosynthetic cycle - hyperammonemia.Selected parameters of nutritional status - determination of albumin concentration. Protein electrophoresis
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Transamination, deamination, urea cycle. Defects of the urosynthetic cycle - hyperammonemia.Selected parameters of nutritional status - determination of albumin concentration. Protein electrophoresis
RNDr. Ivana Matrasová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
14
Monday
14:15 - 15:45
seminar
Amino acid(AA) metabolism and selected disorders of AA metabolism
Mgr. Magdalena Houdová Megová, Ph.D.
Lecture hall 1.26, U nemocnice 4
Tuesday
14:15 - 17:15
practical classes
Control test.
Tetrapyrroles
MUDr. Eva Balážiová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Wednesday
14:15 - 17:15
practical classes
Control test.
Tetrapyrroles
MUDr. Eva Balážiová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Thursday
14:15 - 17:15
practical classes
Control test.
Tetrapyrroles
MUDr. Eva Balážiová, Ph.D.
laboratory of practical lessons, U Nemocnice 5
Friday
14:15 - 15:45
seminar
Amino acid(AA) metabolism and selected disorders of AA metabolism
Mgr. Magdalena Houdová Megová, Ph.D.
Lecture hall 097 – Cori, U nemocnice 5
Schedule block
Week of the block
Day
From - To
Education type
Theme
Teacher
Files
Note
Hodnocení
1
Tuesday
14:15 - 17:15
practical classes
Determination of uric acid
Mgr. Soňa Gyönyörová
Wednesday
14:15 - 17:15
practical classes
Determination of uric acid
Mgr. Soňa Gyönyörová
Thursday
14:15 - 17:15
practical classes
Determination of uric acid
Mgr. Soňa Gyönyörová
2
Tuesday
14:15 - 17:15
practical classes
Calculations in molecular biology
RNDr. Jan Stejskal
Wednesday
14:15 - 17:15
practical classes
Calculations in molecular biology
RNDr. Jan Stejskal
Thursday
14:15 - 17:15
practical classes
Calculations in molecular biology
RNDr. Jan Stejskal
3
Monday
14:15 - 15:45
seminar
Summarizing seminar- structure and function of nucleic acids, RNA types, genome organization, chromatin structure.
Mgr. Magdalena Houdová Megová, Ph.D.
Tuesday
14:15 - 17:15
practical classes
Isolation of genetic material from buccal mucosal swabs. Measurement of isolated DNA concentration.
Wednesday
14:15 - 17:15
practical classes
Isolation of genetic material from buccal mucosal swabs. Measurement of isolated DNA concentration.
Thursday
14:15 - 17:15
practical classes
Isolation of genetic material from buccal mucosal swabs. Measurement of isolated DNA concentration.
Friday
14:15 - 15:45
seminar
Summarizing seminar- structure and function of nucleic acids, RNA types, genome organization, chromatin structure.
Mgr. Magdalena Houdová Megová, Ph.D.
4
Monday
14:15 - 15:45
seminar
Next Generation sequencing
MUDr. František Sedlák, Ph.D.
Tuesday
14:15 - 17:15
practical classes
Electrophoresis of isolated DNA. PCR amplification of the stretch including exon 13 of the DPYD gene.
Wednesday
14:15 - 17:15
practical classes
Electrophoresis of isolated DNA. PCR amplification of the stretch including exon 13 of the DPYD gene.
Thursday
14:15 - 17:15
practical classes
Electrophoresis of isolated DNA. PCR amplification of the stretch including exon 13 of the DPYD gene.
Friday
14:15 - 15:45
seminar
Next Generation sequencing
MUDr. František Sedlák, Ph.D.
5
Monday
14:15 - 15:45
seminar
Regulation of gene expression
RNDr. Jakub Červenka, Ph.D.
Tuesday
14:15 - 17:15
practical classes
PCR electrophoresis and sequence preparation, evaluation in FINCH
Wednesday
14:15 - 17:15
practical classes
PCR electrophoresis and sequence preparation, evaluation in FINCH
Thursday
14:15 - 17:15
practical classes
PCR electrophoresis and sequence preparation, evaluation in FINCH
Friday
14:15 - 15:45
seminar
Regulation of gene expression
RNDr. Jakub Červenka, Ph.D.
6
Tuesday
14:15 - 17:15
practical classes
MB block evaluation
Wednesday
14:15 - 17:15
practical classes
MB block evaluation
Thursday
14:15 - 17:15
practical classes
MB block evaluation
7
Monday
14:15 - 15:45
seminar
Transmembrane transport (proton pump, Wilson's disease, cystic fibrosis)
Friday
14:15 - 15:45
seminar
Transmembrane transport (proton pump, Wilson's disease, cystic fibrosis)
8
Monday
14:15 - 17:15
practical classes
Analysis of urine and urinary sediment in diagnostics
14:15 - 15:45
seminar
Protein folding, ubiquitin proteasome system (misfolding diseases, chaperones, defects in the ubiquitin-proteasome system- VHL, parkin, HPV induced)
doc. MUDr. Petr Bušek, Ph.D.
Tuesday
14:15 - 17:15
practical classes
Analysis of urine and urinary sediment in diagnostics
Wednesday
14:15 - 17:15
practical classes
Analysis of urine and urinary sediment in diagnostics
Thursday
14:15 - 17:15
practical classes
Analysis of urine and urinary sediment in diagnostics
Friday
14:15 - 15:45
seminar
Protein folding, ubiquitin proteasome system (misfolding diseases, chaperones, defects in the ubiquitin-proteasome system- VHL, parkin, HPV induced)
doc. MUDr. Petr Bušek, Ph.D.
9
Monday
14:15 - 15:45
seminar
Preanalytical aspects of biochemical examination
Tuesday
14:15 - 17:15
practical classes
Acid-base balance
MUDr. Eva Balážiová, Ph.D.
Wednesday
14:15 - 17:15
practical classes
Acid-base balance
MUDr. Eva Balážiová, Ph.D.
Thursday
14:15 - 17:15
practical classes
Acid-base balance
MUDr. Eva Balážiová, Ph.D.
Friday
14:15 - 15:45
seminar
Preanalytical aspects of biochemical examination
10
Monday
14:15 - 15:45
seminar
Membrane receptors and their signalling. Cytokines and growth factors (erythropoietin, insulin, VEGF, EGF). Congenital and acquired defects in signalling molecules and cancer
Tuesday
14:15 - 17:15
practical classes
Control test Kidney biochemistry /clearance
RNDr. Ivana Matrasová, Ph.D.
Wednesday
14:15 - 17:15
practical classes
Control test Kidney biochemistry /clearance
RNDr. Ivana Matrasová, Ph.D.
Thursday
14:15 - 17:15
practical classes
Control test Kidney biochemistry /clearance
RNDr. Ivana Matrasová, Ph.D.
Friday
14:15 - 15:45
seminar
Membrane receptors and their signalling. Cytokines and growth factors (erythropoietin, insulin, VEGF, EGF). Congenital and acquired defects in signalling molecules and cancer
11
Monday
14:15 - 15:45
seminar
Membrane receptors and their signalling. Cytokines and growth factors (erythropoietin, insulin, VEGF, EGF). Congenital and acquired defects in signalling molecules and cancer
Tuesday
14:15 - 17:15
practical classes
Examination in patients with diabetes mellitus
Wednesday
14:15 - 17:15
practical classes
Examination in patients with diabetes mellitus
Thursday
14:15 - 17:15
practical classes
Examination in patients with diabetes mellitus
Friday
14:15 - 15:45
seminar
Membrane receptors and their signalling. Cytokines and growth factors (erythropoietin, insulin, VEGF, EGF). Congenital and acquired defects in signalling molecules and cancer
12
Monday
14:15 - 15:45
seminar
Summary seminar - signalling
Tuesday
14:15 - 17:15
practical classes
Xenobiochemistry and toxicology /Ethanol in breath. Detection of drugs in urine
Wednesday
14:15 - 17:15
practical classes
Xenobiochemistry and toxicology /Ethanol in breath. Detection of drugs in urine
Thursday
14:15 - 17:15
practical classes
Xenobiochemistry and toxicology /Ethanol in breath. Detection of drugs in urine
Friday
14:15 - 15:45
seminar
Summary seminar - signalling
13
Monday
14:15 - 15:45
seminar
Summary of energy metabolism control
Tuesday
14:15 - 17:15
practical classes
Biochemistry of muscle tissue/ Determination of selected markers of muscle damage
Wednesday
14:15 - 17:15
practical classes
Biochemistry of muscle tissue/ Determination of selected markers of muscle damage
Thursday
14:15 - 17:15
practical classes
Biochemistry of muscle tissue/ Determination of selected markers of muscle damage
Friday
14:15 - 15:45
seminar
Summary of energy metabolism control
14
Monday
14:15 - 15:45
seminar
Overview of immunological methods- Monoclonal antibodies- preparation and use. Basic immunochemical methods. Immunoturbidimetry, ELISA, RIA.
Tuesday
14:15 - 17:15
practical classes
Immunological methods in clinical practice
Control test
Wednesday
14:15 - 17:15
practical classes
Immunological methods in clinical practice
Control test
Thursday
14:15 - 17:15
practical classes
Immunological methods in clinical practice
Control test
Friday
14:15 - 15:45
seminar
Overview of immunological methods- Monoclonal antibodies- preparation and use. Basic immunochemical methods. Immunoturbidimetry, ELISA, RIA.
Schedule by date
Day
Date
Description
Teacher
Files
Note
Hodnocení
Monday
29.09.2025
Introduction to medicinal chemistry and biochemistry. Water and aqueous dispersion systems.
prof. MUDr. Aleksi Šedo, DrSc.
Thursday
02.10.2025
Acidobasis. pH and buffers, reactions.
Mgr. Lucie Stollinová Šromová, Ph.D.
Monday
06.10.2025
Chemical reactions.
doc. MUDr. Petr Bušek, Ph.D.
Thursday
09.10.2025
Biogenic elements and medically important inorganic compounds.
Mgr. Lucie Stollinová Šromová, Ph.D.
Monday
13.10.2025
Medically important organic compounds.
MUDr. František Sedlák, Ph.D.
Thursday
16.10.2025
Saccharides - overview.
RNDr. Ivana Matrasová, Ph.D.
Monday
20.10.2025
Medically important heterocyclic compounds.
Mgr. Jan Ševčík, Ph.D.
Thursday
23.10.2025
Amino acids and peptides.
doc. MUDr. Petr Bušek, Ph.D.
Monday
27.10.2025
Structure of proteins and their function in the organism.
doc. MUDr. Petr Bušek, Ph.D.
Thursday
30.10.2025
Lipids - an overview.
MUDr. Martin Vejražka, Ph.D.
Monday
03.11.2025
Enzymes 1
prof. MUDr. Aleksi Šedo, DrSc.
Thursday
06.11.2025
Enzymes 2
prof. MUDr. Aleksi Šedo, DrSc.
Monday
10.11.2025
Vitamins
doc. MUDr. Jaromír Křemen, CSc.
Thursday
13.11.2025
introduction to metabolism (biochemistry and energy)
doc. MUDr. Jaromír Křemen, CSc.
Thursday
20.11.2025
Makroergic compounds. Respiratory chain and oxidative phosphorylation.
Metabolism of glycerophospholipids and sphingolipids. Eikosanoids.
MUDr. Martin Vejražka, Ph.D.
Monday
05.01.2026
Protein digestion, transamination, ureosynthetic cycle.
doc. MUDr. Petr Bušek, Ph.D.
Thursday
08.01.2026
Metabolism of the carbon skeleton of amino acids.
doc. MUDr. Petr Bušek, Ph.D.
Monday
12.01.2026
Metabolism of aromatic amino acids and formation of specialized products.
doc. MUDr. Petr Bušek, Ph.D.
Thursday
15.01.2026
Metabolism of tetrapyrroles.
MUDr. Ivan Melezínek, CSc., mimořádný profesor Univerzity Karlovy
Monday
19.01.2026
Purine and pyrimidine metabolism.
doc. MUDr. Jaromír Křemen, CSc.
Schedule by date
Day
Date
Description
Teacher
Files
Note
Hodnocení
Wednesday
25.02.2026
Introduction to Molecular Biology (Biochemistry Information)
Mgr. Martin Sztacho, Ph.D.
Thursday
26.02.2026
Structure and function of nucleic acids, types of RNA molecules
Mgr. Martin Sztacho, Ph.D.
Wednesday
04.03.2026
Organization of prokaryotic, eukaryotic and mitochondrial genomes; structure of chromatin
Mgr. Martin Sztacho, Ph.D.
Thursday
05.03.2026
DNA replication in prokaryotic and eukaryotic cells.
Mgr. Martin Sztacho, Ph.D.
Wednesday
11.03.2026
Mutations and their origin and effects. Mutagens and carcinogens.
Mgr. Helena Kupcová Skalníková, Ph.D.
Thursday
12.03.2026
DNA mutation repair
Mgr. Helena Kupcová Skalníková, Ph.D.
Wednesday
18.03.2026
RNA biosynthesis - DNA transcription, modifications of primary transcript of prokaryotes and eukaryotes, alternative splicing. RNA transport and degradation in eukaryotes.
MUDr. František Sedlák, Ph.D.
Thursday
19.03.2026
Regulation of gene expression.
MUDr. František Sedlák, Ph.D.
Wednesday
25.03.2026
Translation , inhibitors of prokaryotic and eukaryotic translation. Protein folding, chaperones. Post-translational modification of proteins 1.
MUDr. František Sedlák, Ph.D.
Thursday
26.03.2026
Post-translational covalent modification of proteins 2. Sorting and transport of proteins into individual organelles, secretory proteins.
MUDr. František Sedlák, Ph.D.
Wednesday
01.04.2026
Structure and function of cell membranes, cell junctions.
doc. MUDr. Petr Bušek, Ph.D.
Wednesday
08.04.2026
Virus structure, replication and assembly.
doc. MUDr. Jaromír Křemen, CSc.
Wednesday
08.04.2026
Compartmentation of the cell, structure and function of cell organelles, vesicular transport.
MUDr. Martin Vejražka, Ph.D.
Thursday
09.04.2026
Mechanisms of signal transduction 1
prof. MUDr. Aleksi Šedo, DrSc.
Wednesday
15.04.2026
Mechanisms of signal transduction 2
prof. MUDr. Aleksi Šedo, DrSc.
Thursday
16.04.2026
Mechanisms of signal transduction 3
prof. MUDr. Aleksi Šedo, DrSc.
Wednesday
22.04.2026
Biochemistry of peptide and protein hormones and thyroid hormones.
prof. MUDr. Aleksi Šedo, DrSc.
Thursday
23.04.2026
Hormones and signaling pathways affecting energy metabolism.
prof. MUDr. Aleksi Šedo, DrSc.
Wednesday
29.04.2026
Apoptosis
MUDr. Ivan Melezínek, CSc., mimořádný profesor Univerzity Karlovy
Thursday
30.04.2026
Cell cycle
MUDr. Ivan Melezínek, CSc., mimořádný profesor Univerzity Karlovy
Wednesday
06.05.2026
Liver biochemistry
doc. MUDr. Jaromír Křemen, CSc.
Thursday
07.05.2026
Xenobiochemistry
Mgr. Magdalena Houdová Megová, Ph.D.
Thursday
14.05.2026
Biochemistry of blood and hemocoagulation.
doc. MUDr. Petr Bušek, Ph.D.
Wednesday
20.05.2026
Muscle biochemistry and muscle contraction
MUDr. Eva Balážiová, Ph.D.
Thursday
21.05.2026
Humoral immunity
doc. MUDr. Petr Bušek, Ph.D.
Wednesday
27.05.2026
Cellular immunity
doc. MUDr. Petr Bušek, Ph.D.
Thursday
28.05.2026
Biochemistry of neuronal tissue and senses.
MUDr. Eva Balážiová, Ph.D.
Wednesday
03.06.2026
Biochemistry of connective tissue and skin.
MUDr. Ivan Melezínek, CSc., mimořádný profesor Univerzity Karlovy
