Medical Immunology - FG10033

• Title: Medical Immunology
• Guaranteed by: Department of Clinical Immunology and Allergology (15-630)
• Faculty: Faculty of Medicine in Hradec Králové
• Actual: from 2021
• Semester: winter
• Points: 0
• E-Credits: 3
• Examination process: winter s.:
• Hours per week, examination: winter s.:19/18, C+Ex [HS]
• Capacity: unlimited
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Note: deregister from the exam date if a requisite was not fulfilled
• Guarantor: prof. RNDr. Jan Krejsek, CSc.; prof. RNDr. Ctirad Andrýs, Ph.D.
• Pre-requisite : {General Medicine 1st year}
• Incompatibility : FV10029
• Interchangeability : FA0105100, FV10029
• Is incompatible with: FVP028, FV10029
• Is interchangeable with: FVP028, FV10029
• In complex pre-requisite: FG10050, FG10051, FG10052, FG10054, FG10055, FG10056, FG10057, FG10058, FG10059, FG10060, FG10061, FG10062, FG10063, FG10064, FG10065, FG10066, FG10067, FG10068, FG10076, FG10077, FG10078, FG10079, FG10080, FG10081, FG10092, FG10093, FG10094, FG10095, FG10097

Annotation

Fundamental of Medical Immunology is realized in the forms of lectures, seminars and practicals. The aim is to introduce the complex and modern concept of the function of the immune system as principal integrating body system. The emphasize is put on the understanding of the role of cellular substrate of immune response, differentiation of immune cells and description of their function. Special emphasize is given to gain the knowledge of the participation of immune system into the both protective and adverse inflammatory response. Protective inflammatory response against invasion of pathogenic microbes including active immunisation is described. Adverse inflammation is characterized on the molecular and cellular level with the respect to specific characteristic of this type of inflammation in various clinical contexts. Various attempts how to modulate adverse inflammatory response including administration of biologics is described. The basic principal of the interaction between immune system and cancer is given including the role of cancer environment and abuse of immune response is given. Mechanism of action of different biological drugs to overcome cancer cell escape from immune surveillance are described. The primary and secondary immunodeficiencies, including HIV infection and AIDS are presented. The diagnostic procedures and the way how to modulate immune system in immunodeficient patients are given. The basic framework of immunological test used in clinical practice is explained. The indication and interpretation of these immunological tests is given in clinical context of differential diagnostic procedures.

Last update: Kalousová Jana (22.05.2024)

Aim of the course

The complex knowledge of the function of immune system as the principal integral body system and its role in both protective and adverse inflammation is gained in this subject. These informations are used in the context of clinical practice especially in the framework of differential diagnostic procedures. The special emphasize is given to understand modulation of immune system including active immunisation. 

Last update: Kalousová Jana (22.05.2024)

Literature

Compulsory literature

Immunology, 9th Edition. Editoři: D. Male, S. Peebles, V. Male (ClinicalKey Student) https://www.lfhk.cuni.cz/Fakulta/Organizacni-struktura/Domaci-stranky/Lekarska-knihovna/E-knihy/

  

Recomended literature 

1. Krejsek J, Andrýs C, Krčmová I, Imunologie člověka, Garamon, 2016 ISBN 978-80-86472-74-4
2. Murphy K, Casey W, Janeway's Immunobiology, 9th Edition 9th Edition, Kindle Edition 2017, ISBN-13: 978-0815345053

 

Last update: Kalousová Jana (22.05.2024)

Requirements to the exam

Credit

Active participation in practicals and seminars. One seminar could be missed due to illness evidenced by physicion. 

Final multiple choice test passing.

 

Examination

Oral examination. The syllabus is given to students. Fifty questions are generated from this syllabus. Questions consists of part a) and part b). One question is randomly selected by student.

Last update: Kalousová Jana (22.05.2024)

Syllabus

Lectures

• Prof. Dr. Jan Krejsek, Ph.D.
• Prof. Dr. Ctirad Andrys, Ph.D.
• Assoc. Prof. Pavlina Kralickova, MD, Ph.D.
• Dr. Eva Mala, Ph.D.
• Dr. Jakub Novosad, Ph.D.
• Dr. Ondrej Soucek, Ph.D.
• Dr. Vladimira Rezacova, Ph.D.
• Dr. Karolina Jankovicova, Ph.D.
• Dr. Martina Kolackova, Ph.D.

 

 

Lecture

1^st Principles of immune system function (Krejsek)

• genetic background of immune response, epigenetic influences
• natural barriers, microbiota
• cells, tissues and organs of immune system
• immunity as an integral part of protective and damaging inflammatory response

2^nd Innate immunity, basic characteristics (Malá)

• DAMP, PAMP signals, receptors PRR and their function
• initiation and development of the inflammatory response
• humoral mechanisms of innate immunity, interferons, complement system
• cellular mechanisms of innate immunity, phagocytosis, natural cytotoxicity

3^rd Specific immunity (Soucek)

• TcR, BcR receptors
• gene rearrangement
• clonal selection hypothesis of specific immune response
• T cells differentiation
• HLA system, processing and presentation of antigenic fragments to T cells
• polarisation of T cells, Th1, Th2, Th17, Treg subsets of T cells
• B cells differentiation, antigen independent differentiation
• B cell differentiation after antigenic stimulation, isotypic switching, somatic mutation
• antibodies, molecular characteristics, dynamics of antibody production
• immune memory

4^th Mucosal and skin immunity (Novosad, Kolackova)

• barrier function of mucosal surfaces
• microbiota, its contribution to mucosal and systemic immunity
• breast feeding, prebiotics, solid food introduction, probiotics
• nutrition and immunity, proinflammatory activity of adipose tissue
• skin immune system
• skin microbiota

5^th Protective inflammation/damaging inflammation (Malá)

• immune response to viral infections
• immune response to bacterial infections
• immune response to fungal and parasitic infections
• active immunisation, mechanisms of action, adjuvans
• autoimmunity, development
• immunopathology, mechanisms of development, hypersensitivity reactions
• immunopathological diseases, systemic, organ specific
• modulation of inflammation, biological therapy

 

6^th Immunodeficiencie (Kralickova)

• inherited primary immunodeficiencies
• diagnostics, management
• acquired secondary immunodeficiencies
• immunopathogenesis
• treatment

7^th Cancer and inflammatory response, immunomodulation (Soucek)

• protective inflammation is effectively controlling malignant cell proliferation
• some tumours can escape protective inflammation
• immunotherapy in oncology
• immunomodulation, active immunisation

 

 

Lectures

• Prof. Dr. Jan Krejsek, Ph.D.
• Prof. Dr. Ctirad Andrys, Ph.D.
• Assoc. Prof. Pavlina Kralickova, MD, Ph.D.
• Jakub Novosad, MD., Ph.D. 
• Dr. Ondrej Soucek, Ph.D.
• Dr. Vladimira Rezacova, Ph.D.
• Dr. Karolina Jankovicova, Ph.D.

 

Seminars and Practicals

 1^st Allergic inflammation (Kralickova)

• pathophysiology of allergic inflammation
• allergens, components of allergens
• allergic diseases, asthma, rhinitis, eczema …, clinical presentation
• diagnosis of allergic diseases, skin tests, allergen specific IgE, basophil activation test in vitro

2^nd Prevention and treatment of allergic inflammation and anaphylaxis (Kralickova)

• elimination of allergen exposure
• treatment of allergic inflammation
• immunotherapy by allergen
• emergency in allergology, therapeutical options
• angioedemas, hereditary angioedema, diagnostic procedure

3^rd Immune deficiencies (Kralickova)

• primary and secondary immunodeficiencies (including AIDS)
• clinical presentation
• diagnosis, immunological testing
• immunomodulation, immunosubstitution, immunopotentiation

4^th Immunopathology, autoimmunity (Jankovicova, Novosad)

• immunopathology, initiation, pathophysiological mechanisms, hypersensitivity reactions
• immunopathogenesis of selective autoimmune diseases (rheumatoid arthritis, systemic lupus, Crohn disease, celiac disease)
• systemic and organ specific immunopathological diseases
• autoantibodies and other biomarkers in differential diagnosis of immunopathological diseases

5^th Cell mediated immunity (Soucek)

• hybridoma, CD markers, flow cytometry
• routine test of cell mediated immunity (T, B and NK cells)
• tests to determine parameters of phagocytosis and cell proliferation
• immunophenotyping of blood malignancies

6^th Immunological detection of infectious agents (Rezacova)

• direct evidence of infection
• indirect evidence of infection
• detection of specific antibodies as indirect evidence of infection
• detection of specific cell-mediated immune response as indirect evidence of infection (IGRA tests)
• immunological diagnosis of B. pertussis, B. burgdorferi, T. pallidum and M. tuberculosis infections
• clinical interpretation of serological tests

 

 

 

Last update: Kalousová Jana (22.05.2024)

Entry requirements

No prerequisities are required. 

Last update: Kalousová Jana (22.05.2024)