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Poslední úprava: Christian Lanctôt, Dr., Ph.D. (24.10.2016)
nucleus. Although the molecular mechanisms at work in the nucleus are alluded to throughout the course, the emphasis is clearly placed on the cell biological aspects of this key organelle. These aspects are illustrated with recent experimental results taken from the literature, in particular those obtained through observation of single living cells. Broad explanatory concepts are preferred over the description of molecular details. The goal of the course is to present the cell nucleus as a dynamic biological system that is responsible for the expression, the repair and the replication of the genetic information. The integration of these functions within the global cellular context will also be presented. |
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Poslední úprava: Christian Lanctôt, Dr., Ph.D. (11.12.2016)
General textbooks Molecular Cell Biology Essential Cell Biology Cell Biology
Specialized books Genome Organization and Function in the Cell Nucleus
Scientific articles References to 3-4 exhaustive review articles will be given for each lecture. The students are strongly encouraged to read a selection of these in order to complement the lectures. |
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Poslední úprava: Christian Lanctôt, Dr., Ph.D. (24.10.2016)
At the end of the course, an oral exam will take place, during which 2-3 topics that have been covered during the course will be discussed for a total of 20-30 minutes. Students can choose the language (English or Czech) in which they will pass the oral exam. |
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Poslední úprava: Christian Lanctôt, Dr., Ph.D. (24.10.2016)
A. Origin and evolution of the cell nucleus Lecture 1 - Origin and evolution of the cell nucleus
B. Basic concepts and methodological approaches Lecture 2 - Concepts and techniques used in the study of the cell nucleus a. Concepts and models b. Electron and light microscopy c. Biochemical approaches
C. Constituents and structure of the cell nucleus Lecture 3 - Chromatin a. The nucleosome b. The ‘histone code’ c. Non-histone proteins and structural RNAs d. Chromatin structure in vivo Lecture 4 - The nuclear periphery a. The envelope: interaction with cytoplasmic components b. Nuclear pores c. Lamina Lecture 5 - Nuclear bodies a. The nucleolus b. Cajal bodies c. PML bodies d. Speckles e. Paraspeckles
D. The nucleome Lecture 6 - Chromosome territories a. Higher-order organization of chromatin b. Topological domains Lecture 7 - Spatial gene positioning in the nucleus a. The genetic material in three dimensions b. Impact of positioning on nuclear functions Lecture 8 - DNA replication and DNA repair a. Overview of the replication and repair machineries b. Spatial and temporal arrangement of DNA replication c. Preferential sites of DNA repair in the cell nucleus Lecture 9 - Gene regulation a. Overview of the transcriptional machinery b. Transcription factories c. Dynamics of RNA polymerases and transcription factors
E. The living cell nucleus Lecture 10 - Nuclear dynamics a. Self-organization of nuclear compartments b. Chromatin mobility c. mRNA export Lecture 11 - Biophysics of the cell nucleus a. The biophysical properties of chromatin b. Force-generation in the cell nucleus c. Nuclear energetics Lecture 12 - Transcription a. Transcriptional pulsing b. Metabolic regulation of transcription |