RNA structure and function - MB150P91E

• Anglický název: RNA structure and function
• Český název: Struktura a funkce RNA
• Zajišťuje: Katedra buněčné biologie (31-151)
• Fakulta: Přírodovědecká fakulta
• Platnost: od 2014 do 2017
• Semestr: letní
• E-Kredity: 3
• Způsob provedení zkoušky: letní s.:ústní
• Rozsah, examinace: letní s.:2/0, Zk [HT]
• Počet míst: 25
• Minimální obsazenost: neomezen
• 4EU+: ne
• Virtuální mobilita / počet míst pro virtuální mobilitu: ne
• Stav předmětu: vyučován
• Jazyk výuky: angličtina
• Další informace: http://www.img.cas.cz/public/skupiny/Stanek.html
• Poznámka: povolen pro zápis po webu
• Garant: prof. Mgr. David Staněk, Ph.D.
• Vyučující: prof. Mgr. David Staněk, Ph.D.

Anotace

Poslední úprava: prof. Mgr. David Staněk, Ph.D. (31.05.2019)

The course "RNA Structure and Function" is planned for master and PhD students and extends basic molecular and cell biology courses. The course is intended mainly for students who want to extend their knowledge about regulation of gene expression and about the ever-growing world of RNA. Individual lectures are focused on processing of mRNA as well as synthesis, processing and function of non-coding RNAs. The majority of discoveries about RNAs is documented by original scientific publications with explanation of key experiments.

Literatura

Poslední úprava: prof. Mgr. David Staněk, Ph.D. (31.05.2019)

enes VIII, autor: Benjamin Lewin, Publisher: Prentice Hall | ISBN: 0131238264

Molecular Cell Biology. 4th edition. autor: Lodish H, Berk A, Zipursky SL, et al. New York: W. H. Freeman; 2000. ISBN-10: 0-7167-3136-3 (free available on http://www.ncbi.nlm.nih.gov/books/NBK21475/)

RNA World 3rd edition, CSHL Press, ISBN 978-087969739-6

Požadavky ke zkoušce

Poslední úprava: RNDr. Nataša Šebková, Ph.D. (20.02.2018)

Requirements for the exam

3-4 topics presented during the course are discussed during the oral exam. At least three days before the exam students have to submit a short (1-2 pages) assay or review about a topic of their choice from RNA biology. The students can choose the language of the assay (English or Czech) and the same language will be used during the oral exam.

Sylabus

Poslední úprava: RNDr. Nataša Šebková, Ph.D. (20.02.2018)

Sylabus "RNA structure and function"

Introduction

RNA polymerase I transcripts

• ribosomal RNA synthesis and processing, nucleolar structure and function, ribosome structure, and function

RNA polymerase II transcripts

• regulation of transcription through RNA network, connection between transcription and processing

• snRNA and snoRNA biogenesis, scaRNAs. telomerase structure and function

• pre-mRNA processing (capping, splicing, editing, polyA) , alternative splicing, mRNA localization and stability

• cellular structures involved in RNA metabolism

RNA polymerase III transcripts

• transcription, processing and function of tRNAs, 7SK, SRP, U6, RNA P and MRP

Catalytic RNAs

• ribozymes and riboswitches, spliceosome and ribosome as ribozymes

Long non-coding RNAs

• transcription, processing andfunction of HOTAIR, Air, MALAT1 and 2

RNAi

• synthesis, processing and function of miRNA, piwiRNA, siRNA