Last update: RNDr. Hana Konrádová, Ph.D. (10.06.2019)
Lecture: Regeneration capacity of plants, totipotency of plant cell, organ tissue and cell cultures under in vitro, organogenesis, somatic embryogenesis, somaclonal variation, cultivation conditions; plant tissue cultures as a tool for theoretical and practical purposes - micropropagation, production of pathogen free plants, artificial seeds, production of secondary metabolites, biotransformation; plant breeding - protoplast cultures, foreign gene introduction, Agrobacterium system; biotechnology and environment
Laboratory course: Plant multiplication under in vitro, cultivation of organ, tissue and cell cultures, controlled organogenesis on tobaco segments, organogenesis of transgenic plants, somatic embryogenesis in carrot, potato microtuber induction, isolation and cultivation of meristems and zygotic embryos, in vitro cultivation of the plant acording to student's choice.
The course will be taught in English for minimum of 5 students.
Last update: RNDr. Hana Konrádová, Ph.D. (15.03.2019)
Přednáška: Regenerační kapacita rostlin, totipotence rostlinné buňky, somaklonální variabilita, kultivační podmínky, mikropropagace, organogeneze, somatická embryogeneze, využitín kultur rostlinných explantátů.
Praktikum: Množení rostlin v podmínkách in vitro, řízená organogeneze na segmentech, somatická embryogeneze, indukce tvorby mikrohlíz, izolace a kultivace meristemů, in vitro kultivace rostliny dle vlastního výběru.
Doporučuje se ředem absolvovar některou z přednášek Fyziologie rostlin
Literature -
Last update: RNDr. Hana Konrádová, Ph.D. (29.10.2019)
Recent published works are used to prepare the lectures. Students are continuously acquainted with the resources used during the lecture.
Selected information sources:
George E.F., Sherrington, P.D.: Plant Propagation by Tissue Culture, Eastern Press, Eversley,1984
Bajaj, Y.P.S: Biotechnology in Agriculture and Forestry, Vol.2, Springer Verlag,1986
Pierik R.L.M.: In Vitro Cultures of Higher Plants, Martinus Nijhoff Publishers, Dordrecht, Boston, Lancaster, 1987
Bhojwani, S.S.: Development in Crop Science, Plant tissue Culture, Applications and Limitatios, Elsevier, 1990
George E.F.: Plant Propagation by tissue culture, Exergetics Ltd., England, 1993
Son W.-Y., Bhojwani S.S: Morphogenesis in plant tissue cultures. Kluwer Academic Publishers, 1999
Chawla H.S.: Introduction to plant Biotechnology. Science Publishers, Enfield, New Hampshire, USA, 2002
Laimer M., Rücker W.: Plant tissue culture – 100 years since Gottlieb Haberlandt, Springer-Verlag, Wien, 2003
Slater A., Scott N.W., Fowler M.R.: Plant Biotechnology, Oxford University press, 2008
Trigiano N., a Gray D.J.: Plant tissue culture, development and biotechnology, CRCP press, 2011
Last update: RNDr. Hana Konrádová, Ph.D. (15.03.2019)
Studijní literatura:
George E.F., Sherrington, P.D.: Plant Propagation by Tissue Culture, Eastern Press, Eversley,1984 Dixon R.A.: Plant Cell Culture, IRL Press, Oxford, Washington,1985 Bajaj, Y.P.S: Biotechnology in Agriculture and Forestry, Vol.2, Springer Verlag,1986 Pierik R.L.M.: In Vitro Cultures of Higher Plants, Martinus Nijhoff Publishers, Dordrecht, Boston, Lancaster, 1987 Bhojwani, S.S.: Development in Crop Science, Plant tissue Culture, Applications and Limitatios, Elsevier, 1990 Novák F.J.: Explantátové kultury a jejich využití ve šlechtění rostlin, Academia, Praha, 1990 Kováč J.: Explantátové kultury rostlin, skripta, Univerzita Jana Evangelisty Purkyně, 1992 Ondřej M.: Genové inženýrství kulturních rostlin, Academia, Praha, 1992 George E.F.: Plant Propagation by tissue culture, Exergetics Ltd., England, 1993 Son W.-Y., Bhojwani S.S: Morphogenesis in plant tissue cultures. Kluwer Academic Publishers, 1999 Ondřej M. Drobník J.: Transgenoze rostlin, Academia, 2002
Requirements to the exam
Last update: RNDr. Hana Konrádová, Ph.D. (15.03.2019)
Written examination
Syllabus -
Last update: RNDr. Hana Konrádová, Ph.D. (15.03.2019)
History of plant tissue culture techniques Regeneration capacity of plants, totipotency of plant cell - potentials and restrictions Morphogenesis, organogenesis, somatic embryogenesis, pollen embryogenesis, somaclonal variation; Types of cultures - organ, callus, cell and protoplast cultures Cultivation conditions - temperature, light, humidity, cultivation media, aseptic cultivation; choice of mother plants, type of explant Plant tissue culture technique as a tool for plant physiology studies and for practical purposes; Plant micropropagation, production of pathogen-free plants; artificial seeds; Tissue and cell cultures, production of secondary metabolites, bio-transformation; Plant breeding - use of somaclonal variation and screening possibilities of cell cultures; Protoplast cultures, fusion of protoplast, subprotoplasts, transfer of organelles; Transfer of isolated DNA, facilitated endocytosis, microinjection, electroporation, biolistic method; Vector transfer of DNA- Agrobacterium system Plant biotechnology and environmental risks
Last update: RNDr. Hana Konrádová, Ph.D. (15.03.2019)
3. Podmínky kultivace : teplota, světlo, vlhkost, složení kultivačních médií, složení plynné fáze, aseptická kultivace, ošetření mateřských rostlin, typ explantátu
4. Využití explantátů při studiu fyziologie rostlin, vegetativní množení
5. Ozdravování rostlin, umělá semena
6. Tkáňové a buněčné kultury
7. Sekundární metabolity, biotransformace Protoplastové kultury, fúze protoplastů, přenos organel, chromozomů Přímý přenos DNA Agrobacterium jako vektor přenosu DNA Budoucnost a rizika využití transgenních rostlin
