Biochemistry - NBCM012
Title in English: Biochemie
Guaranteed by: Institute of Physics of Charles University (32-FUUK)
Faculty: Faculty of Mathematics and Physics
Actual: from 2015 to 2019
Semester: summer
E-Credits: 4
Hours per week, examination: summer s.:3/0 Ex [hours/week]
Capacity: unlimited
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Guarantor: doc. RNDr. Dana Gášková, CSc.
RNDr. Roman Chaloupka, Ph.D.
Classification: Physics > Biophysics and Chemical Physics
Pre-requisite : NBCM010
Is pre-requisite for: NBCM008
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Annotation -
Last update: prof. RNDr. Marek Procházka, Ph.D. (30.04.2019)
Basic metabolic pathways (biological oxidation, metabolism of sugars, lipids, proteins, photosynthesis, citric acid cycle, regulation of metabolic processes). For 4th year of biophysics study.
Aim of the course -
Last update: GASKOVA/MFF.CUNI.CZ (14.05.2008)

Introduction in Biochemistry

Literature -
Last update: GASKOVA/MFF.CUNI.CZ (14.05.2008)

Lékařská chemie a biochemie (ed. A. Němečková), Avicenum, Praha 1991

P. Karlson: Základy biochemie, Academia, Praha, 1981

J. Musil, O. Nováková: Biochemie v obrazech a schematech , Avicenum, Praha, 1990

J. Koštíř Biochemie známá a neznámá, Avicenum, Praha, 1980

Z. Šípal, P. Anzenbacher, P.Pěč, J. Pospíšil, J. Růžička: Biochemie, SPN, Praha, 1992

D. Voet, J.G. Voetová: Biochemie, Victoria publ., Praha, 1995

Teaching methods -
Last update: GASKOVA/MFF.CUNI.CZ (14.05.2008)

basic course

Syllabus -
Last update: T_FUUK (24.05.2004)

History of biochemistry. Introduction to metabolism: thermodynamics of living systems, metabolic pathways, experimental approaches to metabolic studies, thermodynamics of phosphate compounds, oxidation-reduction reactions.

Saccharide metabolism. Glycolysis:The glycolytic pathway, Glycolytic reactions, Fermentation ? anaerobic pyruvate degradation, Regulation of metabolic flux, Metabolism of other hexoses.

Other pathways of saccharide metabolism. Gluconeogenesis, Glyoxylate pathway, Biosynthesis of oligosaccharides and glycoproteins, Pentose cycle.

Citrate cycle. Overview, Metabolic sources of acetyl-coenzyme A, Citrate cycle enzymes, Citrate cycle regulation, Amphibolic nature of the citrate cycle.

Membrane transport.Transport thermodynamics, Transport kinetics and mechanisms, Primary active transport, Secondary active transport.

Electron transport and oxidative phosphorylation. Mitochondria, Electron transport, Oxidative phosphorylation, Regulation of ATP production.

Photosynthesis. Chloroplasts, Light-dependent reactions, Reactions taking place without direct participation of light.

Expression and transfer of genetic information. Replication: DNA ? the carrier of genetic information, DNA replication: an overview, Replication enzymes. Transcription: Role of RNA in protein synthesis, RNA polymerase, Transcription regulation, Post-transcription modifications. Translation: Genetic code, Transfer RNA, Ribosomes, Translation regulation, Post-translation modifications.