SubjectsSubjects(version: 916)
Course, academic year 2022/2023
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Molecular Applications in Zoology - MB170P62
Title: Molecular Applications in Zoology
Czech title: Molekulární metody v zoologii
Guaranteed by: Department of Zoology (31-170)
Faculty: Faculty of Science
Actual: from 2022
Semester: summer
E-Credits: 4
Examination process: summer s.:
Hours per week, examination: summer s.:2/0, Ex [HT]
Extent per academic year: 1 [days]
Capacity: unlimited
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: English
Additional information: http://zuzanastarostova.webnode.cz/news/molecular-applications-in-zoology/
Note: enabled for web enrollment
Guarantor: Mgr. Zuzana Starostová, Ph.D.
Teacher(s): doc. RNDr. Pavel Hulva, Ph.D.
doc. Mgr. Pavel Munclinger, Ph.D.
RNDr. Radka Reifová, Ph.D.
Mgr. Zuzana Starostová, Ph.D.
RNDr. František Šťáhlavský, Ph.D.
doc. RNDr. Michal Vinkler, Ph.D.
Annotation -
Last update: Mgr. Zuzana Starostová, Ph.D. (11.01.2023)
Molecular markers and use of sophisticated cytogenetic techniques have opened new windows through which to view the
natural biological world. This course aims to address basic and advanced applications of these genetic markers in
resolving tasks of current zoology (e.g. population biology, organismal evolution, phylogeny or phylogeography). We will
introduce various laboratory techniques for revealing molecular markers as well as several cytogenetic techniques and
document their use in interesting zoological case studies. We will cover areas ranging from assessments of genetic
parentage and kinship in the most recent generations to phylogenetic assessments in the animal tree of life, as well as
studying ecology and life-history of species. We will show examples ranging from single gene studies to genomic
approach. The course includes also two half days of practicals (lab and computers).

The first lecture will be on Tuesday February 14, 2023. Lectures are planned for Tuesdays at 1.10 PM (13:10) in the room B312 (3rd floor, Viničná 7). Study material can be found also on webpage: https://zuzanastarostova.webnode.cz/news/molecular-applications-in-zoology/.
Literature -
Last update: Mgr. Zuzana Starostová, Ph.D. (20.09.2014)

For the PowerPoint slides and other information related to the course please see: http://zuzanastarostova.webnode.cz/news/molecular-applications-in-zoology/

Requirements to the exam -
Last update: Mgr. Zuzana Starostová, Ph.D. (08.02.2022)

Written exam covering the range of topics included in the syllabus (one question per lesson). The form of exam could change (to oral or online) due to Covid-19 situation and rules valid during the examination period. Participation at the practicals is compulsory. Details about practicals will be specified by email.

Syllabus -
Last update: Mgr. Zuzana Starostová, Ph.D. (30.01.2023)

1    Introduction, DNA isolation, PCR and DNA sequencing, basics in sequence data handling (lecturer Zuzana Starostová)
2    Next generation sequencing – introduction, short-read and long-read sequencing methods, assemblies (lecturer Radka Reifová)
3    How to read and make phylogenetic trees (lecturer Zuzana Starostová)
4    Use of molecular phylogetics in zoology (lecturer Zuzana Starostová)
5    Molecular phylogeography (lecturer Pavel Hulva)
6    Microsatellites and molecular identification of species, individuals and sex (lecturer Pavel Munclinger)
7    Population structure and paternity studies (lecturer Pavel Munclinger)
8    Next generation sequencing – applications: whole genome sequecing, exome sequencing, ddRAD sequencing, metagenomics (lecturer Radka Reifová)
9    Gene expression – quantitative PCR, expression microarrays, transcriptome sequencing and analysis, epigenomics (lecturer Radka Reifová)
10    Functional genetic variability: From SNP to selection (lecturer Michal Vinkler)
11    Cytogenetic methods and their application in zoology (lecturer František Šťáhlavský)

Practicals:
Day 1: computers - database search and basic DNA sequence data handling

Day 2: lab work - molecular method of sex determination in birds - DNA isolation, PCR, gel electrophoresis

 
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