SubjectsSubjects(version: 850)
Course, academic year 2019/2020
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Molecular mechanisms of apoptosis - MB150P79E
Title in English: Molecular mechanisms of apoptosis
Czech title: Molekulární mechanismy apoptózy
Guaranteed by: Department of Cell Biology (31-151)
Faculty: Faculty of Science
Actual: from 2016
Semester: winter
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Capacity: unlimited
Min. number of students: unlimited
State of the course: taught
Language: English
Guarantor: RNDr. Ladislav Anděra, CSc.
Teacher(s): RNDr. Ladislav Anděra, CSc.
Incompatibility : MB150P79
Is incompatible with: MB150P79
Last update: doc. RNDr. František Půta, CSc. (07.02.2018)

The lecture course is mainly focused on the presentation of various mechanisms of activation and modulation of regulated cell death (apoptosis, regulated necrosis, etc.) predominantly in mammalian (mouse and human) cells/organisms and on cell death-related processes. In addition to mammalian cells the mechanisms of regulated cell death will be addressed also in cells of other vertebrates, invertebrates, single cell organisms and plants. Lastly, role or importance of regulated cell death in a number of physiological and pathophysiological processes occurring in organisms is shown and methods of detection and quantification of cell death are presented.
Literature -
Last update: doc. RNDr. František Půta, CSc. (07.02.2018)

The lectures in Powerpoint are accesible on internet (web address and access will be specified in the first lecture). On the same web address will be also accessible for downloading recent reviews and breakthrough primary paper related to the subject of respective lecture. 

Requirements to the exam -
Last update: Mgr. Naděžda Brdičková, Ph.D. (11.04.2012)

Written exam consists of two blocks - 1) 15-20 questions (possible answers a-d, 0-4 correct answers) and 2) 10-15 questions (students write their own short answers). At least 70% of the maximum number of points is required to successfully pass the exam. There is no time limit. Oral exam is also possible if necessary.

Last update: doc. RNDr. František Půta, CSc. (07.02.2018)

1. Definition of programmed or regulated cell death, its historical and evolution-related aspects and importance for life of an organism. Overview of principles and signalling in various forms of regulated cell death.

2. Caspase-dependent apoptosis: Initiator and effector caspases, their activation, regulation and function.

3. Mitochondria and other organelles in apoptosis; proteins from Bcl-2 family.

4. Intracellular or intrinsic induction of apoptosis, stress response, p53 tumour suppressor.

5. Extracellular or extrinsic induction of apoptosis (death receptors, immune system etc.).

6. Regulated necrosis and other mechanisms of regulated cell death.  

7. Final steps in cell destruction, removal of the corpses.

8. Summary of activation and regulation of cell death in mammalian cells.

9. Regulated cell death in other vertebrates and in invertebrates.

10. Regulated cell death in single cell organisms and in plants.

11. Physiology and pathophysiology of regulated cell death; methods of cell death detection and quantification. 

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