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Course, academic year 2019/2020
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Histology - A Practical Course - MB150C27E
Title in English: Histology - A Practical Course
Czech title: Histologie - praktická cvičení
Guaranteed by: Department of Cell Biology (31-151)
Faculty: Faculty of Science
Actual: from 2019
Semester: summer
E-Credits: 1
Examination process: summer s.:
Hours per week, examination: summer s.:0/2 C [days/semester]
Capacity: unlimited
Min. number of students: unlimited
State of the course: taught
Language: English
Guarantor: Mgr. Lenka Doubravská, Ph.D.
Mgr. Valéria Grobárová, Ph.D.
Incompatibility : MB150C27A, MB150C27U
Is incompatible with: MB150C27A
Annotation -
Last update: Mgr. Lenka Doubravská, Ph.D. (15.03.2019)
Designed for those interested in histological methodologies, suitable as a supplement lectures on histology. Recommended to take the course first year of bc. study after finishing the Practicals of Cell Biology. This is a basic course of histological techniques, where students learn about preparing smearing, stemping and cutting types of histological specimens, including fluorescent variant of staining.
Literature -
Last update: Mgr. Lenka Doubravská, Ph.D. (14.03.2019)
For this practical is not required any special literature, protocols are provided. At the beginning of the practical exercises all issues are thoroughly discussed and all protocoarer are available.
Requirements to the exam
Last update: Mgr. Lenka Doubravská, Ph.D. (14.03.2019)

For getting the credits the 100% attendance is obligatory. Students have to prepare set of required specimens and their images using a microscope equipped with a color camera with high resolution - both in the fluorescence mode and in transmitted light.

Syllabus
Last update: Mgr. Lenka Doubravská, Ph.D. (14.03.2019)

1. Preparation of histological specimens: the procurement of tissues and organs (mouse), fixation, drainage using  ascending series of alcohol, embedding in paraffin, cutting on a microtome, staining with hematoxylin / eosin, closing in Canadian balsam.
2. Preparation of smearing specimens of blood and bone marrow: methanol fixation, Giemsa
3. Preparation of stamping specimens of selected organs - spleen, thymus, lymph nodes, testes, cerebellum fixation with methanol, Giemsa
4. Preparation of fluorescently labeled specimens (detection of plasma cells in the spleen,  characterization of the developmental stages of spermatogenesis in the testis)
5. Preparation of immunofluorescent slides for fluorescence microscopy (fixation, permeabilization blocking, staining with primary and secondary antibodies, DAPI, embeding in Mowiol)

 
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