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Course, academic year 2023/2024
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Practical course of plant physiology - MB130C14A
Title: Praktikum z fyziologie rostlin
Czech title: Praktikum z fyziologie rostlin
Guaranteed by: Department of Experimental Plant Biology (31-130)
Faculty: Faculty of Science
Actual: from 2019
Semester: summer
E-Credits: 3
Examination process: summer s.:
Hours per week, examination: summer s.:0/1, C [TS]
Capacity: 140
Min. number of students: 5
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Additional information:
Note: enabled for web enrollment
Guarantor: RNDr. Michal Hála, Ph.D.
Teacher(s): Mgr. Vojtěch Čermák, Ph.D.
Rajdeep Ghosh, M.Sc.
RNDr. Michal Hála, Ph.D.
Mgr. Eliška Kobercová
RNDr. Jan Konečný
RNDr. Hana Konrádová, Ph.D.
Mgr. Pavel Krupař
RNDr. Petra Mašková, Ph.D.
Mgr. Andrea Moravcová
Mgr. Jakub Skřivánek
Mgr. Beáta Soperová
Mgr. Andrea Zounková
Class: Portable Photosynthesis System
Zařízení na měření vodního potenciálu - Schollande
Přenosný senzor pro měření rostlinných barviv
Incompatibility : MB130C14, MB130C74, MB130P13
Is incompatible with: MB130P13, MB130C74
Annotation -
Last update: RNDr. Hana Konrádová, Ph.D. (29.10.2019)
Practical course deals with plant pigments, photosynthesis and respiration, mineral nutrition, water regime and phytohormone
effects. Student gets knowledge on plant functions and acquires experience on work with living plant material, explant in
vitro cultivation, meets basic analytical methods , eg. chromatography, spectrophotometry and gasometry.
Literature -
Last update: RNDr. Hana Konrádová, Ph.D. (29.10.2019)

Description of goals, methods and background informations are available in a printed form in the practicum.

Requirements to the exam - Czech
Last update: RNDr. Michal Hála, Ph.D. (12.03.2019)

účast na všech úlohách, odevzdání protokolů dle stanovených požadavků

Syllabus -
Last update: RNDr. Michal Hála, Ph.D. (12.03.2019)

Plant pigments. Extraction and TL chromatography of chloroplast pigments, elution and determination of absorption spectra. transport of electron from donor to acceptor in prepared extract. Paper chromatography of vacuolar pigments, identification by means of UV-source.

Photosynthesis, respiration, assimilates. Measurement of net photosynthesis in C3 and C4 plants estimating changing level of carbon dioxide in closed system. Influence of CO2 level on rate of photosynthesis, compensation concentration of CO2. Rate of respiration measured as increasing level of CO2 in darkness in closed system. Reducing and non-reducing sacharides,
aldo-sugars, keto-sugars. Storage polysacharides. Starch localisation in leaves - function.

Plant water regime. Estimation of osmotic potential of potato tuber tissue (gravimetric method) and epidermis of onion bulb (observation of incipient plasmolysis). Rate of transpiration, stomatal and cuticular transpiration - gravimetric method. Root pressure - gravimetric method. Light and stomata opening and closure.

Mineral nutrition. Nitrogen deficiency, effect on growth, development and efficiency of photosythetic apparatus in young plants of maize. N, P, K, Mg, Ca deficiency - manifestation in young plants of wheat and kohlrabi. Fytotrof - an expert system for mineral deficiency assessment.

Plant in vitro cultivation. Passage of Drosera. Potato micropropagation by node segments. Cell culture - cell viability estimation using trypane blue.

Plant growth substances. Effect of IAA, BAP, ABA and GA3 on growth and development of cress seedlings.

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