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Course, academic year 2023/2024
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Practical course of plant physiology - MB130C14
Title: Praktikum z fyziologie rostlin
Czech title: Praktikum z fyziologie rostlin
Guaranteed by: Department of Experimental Plant Biology (31-130)
Faculty: Faculty of Science
Actual: from 2021
Semester: summer
E-Credits: 2
Examination process: summer s.:
Hours per week, examination: summer s.:0/1, C [TS]
Capacity: unlimited
Min. number of students: 5
4EU+: no
Virtual mobility / capacity: no
State of the course: cancelled
Language: Czech
Additional information:
Note: enabled for web enrollment
Guarantor: RNDr. Michal Hála, Ph.D.
Incompatibility : MB130P13
Is incompatible with: MB130C14A, MB130C14E
Is pre-requisite for: MB130P18E, MB130P18
Opinion survey results   Examination dates   Schedule   
Annotation - Czech
Praktická výuka fyziologie rostlin. Zabývá se rostlinnými barvivy, fotosyntézou a respirací, minerální výživou, vodním režimem a efekty růstových látek. Student si osvojí základní návyky práce s živým rostlinným materiálem, seznámí se s kultivací rostlinných explantátů in vitro a s některými základními analytickými metodami, např. chromatografií, spektrometrií a gazometrií.
Last update: VOTRUB (22.04.2005)
Literature - Czech

Dvořák P.: Praktikum z fyziologie rostlin. SPN Praha1984. Str. 97. 17-251-85

Last update: LIPAVSKA (19.04.2007)
Requirements to the exam - Czech

účast na všech úlohách, odevzdání protokolů dle stanovených požadavků,

Last update: Lipavská Helena, doc. RNDr., Ph.D. (05.04.2012)
Syllabus -

Plant pigments. Extraction and TL chromatography of chloroplast pigments, elution and determination of absorption spectra. transport of electron from donor to acceptor in prepared extract. Paper chromatography of vacuolar pigments, identification by means of UV-source.

Photosynthesis, respiration, assimilates. Measurement of net photosynthesis in C3 and C4 plants estimating changing level of carbon dioxide in closed system. Influence of CO2 level on rate of photosynthesis, compensation concentration of CO2. Rate of respiration measured as increasing level of CO2 in darkness in closed system. Reducing and non-reducing sacharides,

aldo-sugars, keto-sugars. Storage polysacharides. Starch localisation in leaves - function.

Plant water regime. Estimation of osmotic potential of potato tuber tissue (gravimetric method) and epidermis of onion bulb (observation of incipient plasmolysis). Rate of transpiration, stomatal and cuticular transpiration - gravimetric method. Root pressure - gravimetric method. Light and stomata opening and closure.

Mineral nutrition. Nitrogen deficiency, effect on growth, development and efficiency of photosythetic apparatus in young plants of maize. N, P, K, Mg, Ca deficiency - manifestation in young plants of wheat and kohlrabi. Fytotrof - an expert system for mineral deficiency assessment.

Plant in vitro cultivation. Passage of Drosera. Potato micropropagation by node segments. Cell culture - cell viability estimation using trypane blue.

Plant growth substances. Effect of IAA, BAP, ABA and GA3 on growth and development of cress seedlings.

Last update: VOTRUB (26.04.2005)
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