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Microbiology - Practical Classes - GAF397

Title:	Microbiology - Practical Classes
Form of teaching:	lecture+seminar+practical classes
Guaranteed by:	Department of Biomedical Sciences (16-16150)
Faculty:	Faculty of Pharmacy in Hradec Králové
Actual:	from 2026
Duration in semesters:	1
Semester:	summer
Points:	0
E-Credits:	2
Examination process:	summer s.:
Hours per week, examination:	summer s.:0/21, C [HS]
Capacity:	unknown / unknown (20)
Maximum number of enrolled students:	20
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
Key competences:
State of the course:	taught
Language:	English
Teaching methods:	full-time
Level:
Repeated enrollment:	1 / 1 / - / 1
Note:	course can be enrolled in outside the study plan enabled for web enrollment

Guarantor:	RNDr. Klára Konečná, Ph.D.
Co-requisite :	GAF433
Incompatibility :	GAF371
Interchangeability :	GAF371

Annotation -

The subject Microbiology -practical classes is a follow-up subject for the subject Microbiology. Emphasis in this course is primarily placed on methodological approaches chosen in microbiological laboratories. During the study of this course, the student will gain an overview and practical skills regarding methodologies that relate to both the issue of pharmaceutical microbiology and medical microbiology. The aim of the course is to prepare students to develop the knowledge gained during further study and apply it in medical practice.

Last update: Konečná Klára, RNDr., Ph.D. (02.09.2025)

Course completion requirements -

Credit conditions:
- 100% attendance in laboratory practice (no unexcused absence)-specified in more details in the folder Study materials.

- Average mark from protocols less than / equal to 2.0. Protocols submitted no later than the end of June.

- Successfully passed credit test.

Last update: Konečná Klára, RNDr., Ph.D. (02.09.2025)

Literature -

Obligatory:

. . In Emanuel Goldman, Lorrence H Green. Practical Handbook of Microbiology . : Routledge Handbooks Online, 2015, s. -. ISBN 9781466587397..

Last update: Konečná Klára, RNDr., Ph.D. (02.09.2025)

Teaching methods -

- PPT presentations, seminars – discussion on given topic

- practical classes – introduction to basic methods used in microbiological laboratories

Last update: Konečná Klára, RNDr., Ph.D. (02.09.2025)

Course assessment methods and requirements for successful completion, grading scheme -

Knowledge to the methodical approaches applied in the microbiological laboratories

- disinfection, sterilization, microbiological control of environmental cleanliness

- microscopic techniques, native and stained mounts, staining methods

- cultivation, cultivation conditions, types of cultivation media, use of cultivation media, inoculation of cultivation media

- testing the susceptibility of microorganisms to antimicrobials

- laboratory diagnostics of gram-positive, gram-negative bacteria

- laboratory diagnostics of fungal agents

- laboratory diagnostics of parasitic agents

- methods for identification of microbial agents

Knowledge defined by questions within the learning outcomes documents

Last update: Konečná Klára, RNDr., Ph.D. (02.09.2025)

Syllabus -

Methodical approaches in the microbiological laboratory

- disinfection, sterilization, swab methods

- microscopic techniques, native and stained mounts, staining methods

- cultivation, cultivation conditions, types of cultivation media, use of cultivation media

- testing the susceptibility of microorganisms to antimicrobials

- laboratory diagnostics of gram-positive, gram-negative bacteria

- laboratory diagnostics of fungal agents

- laboratory diagnostics of parasitic agents

- methods for identification of microbial agents

Last update: Konečná Klára, RNDr., Ph.D. (02.09.2025)

Learning resources -

Study Materials | Intranet FAF (cuni.cz)

Department of Biological and Medical Sciences | Intranet FAF (cuni.cz)

Last update: Konečná Klára, RNDr., Ph.D. (02.09.2025)

Learning outcomes -

Learning Outcomes of the subject Microbiology - Practical Classes

Upon completion of the course, the student will be able to:

- Explain and apply the principles of sterilization and disinfection, distinguish naturally sterile and non-sterile clinical specimens, understand biosafety levels (BSL), and work safety with a laminar flow cabinet.

- Assess microbial contamination of environments and surfaces using swab methods and evaluate the correctness of hand-disinfection procedures.

- Perform basic microscopic techniques, including preparation of wet mounts and staining procedures, and identify microorganisms using Gram staining method.

- Describe cultivation principles, select appropriate cultivation conditions, characterize microbial colonies macroscopically, and perform basic biochemical tests (catalase, oxidase, KOH test).

- Define the term antibiotic drug, classify antimicrobial susceptibility testing methods, and explain shortcuts MIC and MBC.

- Evaluate antimicrobial susceptibility using disk diffusion method and broth microdilution methods, including interpretation in accordance with EUCAST guidelines.

- Identify clinically relevant Gram-positive and Gram-negative cocci and rods, and apply biochemical identification techniques, and yeast identification using auxanograms.

- Apply basic diagnostic approaches for fungal and parasitic infections, including cultivation of fungi, microscopic identification of fungal agents, and detection of helminth infections.

t/m

Last update: Konečná Klára, RNDr., Ph.D. (02.12.2025)