Molecular Biology and Genetics - GAF370

• Title: Molecular Biology and Genetics
• Guaranteed by: Department of Biochemical Sciences (16-16160)
• Faculty: Faculty of Pharmacy in Hradec Králové
• Actual: from 2021
• Semester: summer
• Points: 0
• E-Credits: 4
• Examination process: summer s.:written
• Hours per week, examination: summer s.:42/0, Ex [HS]
• Capacity: unknown / unknown (unknown)
• Min. number of students: unlimited
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Note: deregister from the exam date if a requisite was not fulfilled; course can be enrolled in outside the study plan; enabled for web enrollment
• Guarantor: prof. PharmDr. Tomáš Šimůnek, Ph.D.
• Comes under: 2.ročník 2023/24 AJ
• Co-requisite : GAF141
• Incompatibility : GAF296
• Interchangeability : GAF296
• Is co-requisite for: GAF332, GAF231
• Is pre-requisite for: GAF339, GAF305

Annotation

Last update: prof. PharmDr. Tomáš Šimůnek, Ph.D. (19.10.2021)

Molecular biology deals with the study of the cellular processes on molecular level. Genetics is concerned with the study of genes, genetic variation, and heredity in organisms and populations.The main focus of this subject is description of heredity-related biological macromolecules (DNA, RNA, proteins), their mutual interactions and regulation of their function. Furthermore, the subject scope is extended towards explanation of the most important experimental molecular techniques, basics of clinical genetics and molecular diagnostics, as well as application of genetic engineering (recombinant DNA technology).

Course completion requirements

Last update: prof. PharmDr. Tomáš Šimůnek, Ph.D. (19.10.2021)

Written test, consists of two parts

 Part A: 30 statements - to distinguish between correct and incorrect (maximum 30 points)

 Part B: 5 topics/questions to answer by writing (maximum 15 points)

Literature

Last update: prof. PharmDr. Tomáš Šimůnek, Ph.D. (19.10.2021)

Obligatory:

• . . In Alberts, Bruce, Hunt, Tim, Johnson, Alexander Lewis, Julian, Morgan, David Owen, Raff, Martin C., Roberts, Keith et al.. Molecular biology of the cell . : , 2015, s. -. ISBN 978-1-315-73536-8..

Syllabus

Last update: prof. PharmDr. Tomáš Šimůnek, Ph.D. (19.10.2021)

Lectures

Model organisms in molecular biology

Structure and organisation of prokaryotic and eukaryotic genomes

DNA replication

Heredity

Population genetics

Genetic mutations

Genetic repair, recombination

Structure and types of RNA

Transcription + posttranscriptional modifications of RNA

Genetic code, ribosomes, translation

Posttranslational modifications, protein degradation

Regulation of gene expression in prokaryotes

Regulation of gene expression in and eukaryotes

Epigenetic gerulations

Isolation of nucleic acids

Overview of enzymes used in experimental molecular biology

Polymerase chain reaction and its modifivations, real-time PCR

Gel electrophoresis

DNA sequencing

Hybridisation, blotting

Molecular cloning, methods of gene transfer

Hereditary diseases

Molecular diagnostics

Introduction to proteomics

Bioinformatics