Xenobiochemistry - Practical Training - GAF361

• Title: Xenobiochemistry - Practical Training
• Guaranteed by: Department of Biochemical Sciences (16-16160)
• Faculty: Faculty of Pharmacy in Hradec Králové
• Actual: from 2021
• Semester: summer
• Points: 0
• E-Credits: 2
• Examination process: summer s.:
• Hours per week, examination: summer s.:0/28, C [HS]
• Capacity: unknown / unknown (unknown)
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Guarantor: prof. Ing. Vladimír Wsól, Ph.D.
• Co-requisite : GAF237
• Incompatibility : GAF267
• Interchangeability : GAF267
• Is interchangeable with: GAF267

Annotation

English

Xenobiochemistry studies the metabolic transformations of substances that are foreign to organisms, especially drugs and environmental contaminants. In Practical Exercises in Xenobiochemistry, students will get acquainted with basic model systems and methods in the study of xenobiotic metabolism, biotransformation enzymes, and factors that affect the metabolism of xenobiotics.

Last update: Wsól Vladimír, prof. Ing., Ph.D. (16.09.2024)

Czech

Xenobiochemie studuje metabolické přeměny látek, které jsou organismům cizí, zvláště léků a kontaminantů životního prostředí. V Praktických cvičeních z xenobiochemie se studenti seznámí se základními modelovými systémy a metodami při studiu metabolismu xenobiotik, biotransformačních enzymů a faktorů, které metabolismus xenobiotik ovlivňují.

Last update: Wsól Vladimír, prof. Ing., Ph.D. (16.09.2024)

Course completion requirements

For the credit, it is necessary to complete all practical tasks successfully. 

Last update: Wsól Vladimír, prof. Ing., Ph.D. (16.09.2024)

Literature

Obligatory:

• Gibson, G. Gordon Skett, Paul. Introduction to drug metabolism. Cheltenham, UK: Nelson Thornes Publishers, 2001, 256 s. ISBN 0-7487-6011-3.
• Anzenbacher, Pavel, Zanger, Ulrich M. (eds.). Metabolism of Drugs and other Xenobiotics. Weinheim: Wiley, 2012, 724 s. ISBN 978-3-527-32903-8.
• Nassar, Ala F. Hollenberg, Paul F. Scatina, JoAnn (eds.). Drug metabolism handbook : concepts and applications. Hoboken, N.J.: Wiley, 2009, 1041 s. ISBN 978-0-470-11803-0.

Last update: prepocet_literatura.php (19.09.2024)

Syllabus

English

Biotransformation study of a model drug in vitro

Determination of specific activities of the selected aldo-keto reductases

Electrophoresis and immunodetection of microsomal proteins

Cytotoxicity test of selected drugs on the cell line

Overall evaluation of the results obtained

Last update: Wsól Vladimír, prof. Ing., Ph.D. (16.09.2024)

Czech

Stanovení specifických aktivit některých aldo-keto reduktas

Biotransformační studie modelového léčiva in vitro, TLC

Elektroforéza a imunodetekce mikrosomálních proteinů

Test cytotoxicity vybraných léčiv na buněčné linii

Celkové hodnocení získaných výsledků

Last update: Wsól Vladimír, prof. Ing., Ph.D. (16.09.2024)

Learning outcomes

The course Xenobiochemistry – Practical Training builds upon the knowledge acquired in the course Xenobiochemistry.
After completing the course, students will gain essential laboratory skills in the field of drug metabolism. The focus will be primarily on incubation studies of selected drugs with recombinant enzymes and selected cell lines, evaluation of these studies using high-performance liquid chromatography (HPLC), and cytotoxicity testing.

Learning Outcomes:

Based on the acquired knowledge and skills, students will be able to:

·         Prepare all components of the incubation mixture in required concentrations;

·         Set up incubation schemes with recombinant proteins for all required experiments, including control samples;

·         Handle and work with cell lines;

·         Prepare samples for HPLC analysis using the appropriate procedures;

·         Evaluate analyzed samples and calculate the relevant enzyme activities;

·         Design, conduct, and interpret cytotoxicity studies of drugs.

Last update: Wsól Vladimír, prof. Ing., Ph.D. (12.06.2025)