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Course, academic year 2023/2024
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Xenobiochemistry - Practical Training - GAF361
Title: Xenobiochemistry - Practical Training
Guaranteed by: Department of Biochemical Sciences (16-16160)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2021
Semester: summer
Points: 0
E-Credits: 2
Examination process: summer s.:
Hours per week, examination: summer s.:0/28, C [HS]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Virtual mobility / capacity: no
Key competences:  
State of the course: taught
Language: English
Teaching methods: full-time
Teaching methods: full-time
Guarantor: prof. Ing. Vladimír Wsól, Ph.D.
Co-requisite : GAF237
Incompatibility : GAF267
Interchangeability : GAF267
Is interchangeable with: GAF267
Annotation -
Last update: prof. Ing. Vladimír Wsól, Ph.D. (27.09.2023)
Xenobiochemistry studies the metabolic transformations of substances that are foreign to organisms, especially drugs and environmental contaminants. In Practical Exercises in Xenobiochemistry, students will get acquainted with basic model systems and methods in the study of xenobiotic metabolism, biotransformation enzymes, and factors that affect the metabolism of xenobiotics.
Course completion requirements
Last update: prof. Ing. Vladimír Wsól, Ph.D. (27.09.2023)

For the credit, it is necessary to complete all practical tasks successfully. 

Last update: prof. Ing. Vladimír Wsól, Ph.D. (27.09.2023)


  • Gibson, G. Gordon Skett, Paul. Introduction to drug metabolism. Cheltenham, UK: Nelson Thornes Publishers, 2001, 256 s. ISBN 0-7487-6011-3.
  • Anzenbacher, Pavel, Zanger, Ulrich M. (eds.). Metabolism of Drugs and other Xenobiotics. Weinheim: Wiley, 2012, 724 s. ISBN 978-3-527-32903-8.
  • Nassar, Ala F. Hollenberg, Paul F. Scatina, JoAnn (eds.). Drug metabolism handbook : concepts and applications. Hoboken, N.J.: Wiley, 2009, 1041 s. ISBN 978-0-470-11803-0.

Syllabus -
Last update: prof. Ing. Vladimír Wsól, Ph.D. (27.09.2023)

Biotransformation study of a model drug in vitro

Determination of specific activities of the selected aldo-keto reductases

Electrophoresis and immunodetection of microsomal proteins

Cytotoxicity test of selected drugs on the cell line

Overall evaluation of the results obtained

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