Last update: doc. MUDr. Alena Stoklasová, CSc. (22.01.2020)
Biochemistry: An introduction to enzymology, overview of intermediary metabolism, biological oxidation, the citric acid cycle, metabolism of carbohydrates, lipids, eicosanoids and steroids, ketogenesis, metabolism of proteins and amino acids, metabolism of porphyrins and bile pigments. Metabolism of tissues: blood, water and ions metabolism, acid-base balance, haemostasis and blood clotting, contractile and structural proteins, metabolism of connective tissue; bones, teeth - composition, mineralization, saliva, neurotransmitters, hormones, introduction to xenobiochemistry and immunochemistry. Focused on dental topics.
Requirements to the exam
Last update: doc. MUDr. Alena Stoklasová, CSc. (03.02.2019)
Credit
a) Attendance at laboratory classes and seminars (3 absences will be tolerated).
b) Complete protocols.
c) At least pass average (3.00) in written tests and oral examinations. Substitution of test you miss is necessary to discuss with doc. A. Stoklasová.
Examination
The examination consists of two parts - multiple choice questionnaire test and oral examination - and it is conditioned with having both credits. Written test: at least 11 points are necessary for oral part of examination (32 points max.). The students who fail to satisfy the requirements of the examination are re-examined orally (two possibilities).
Syllabus
Last update: doc. MUDr. Alena Stoklasová, CSc. (22.01.2020)
Lectures
Introduction to Metabolism of Proteins and Amino Acids. Transamination, Oxidative Deamination and Decarboxylation of Amino Acids. Catabolism of the Carbon Skeleton of Amino Acids. Ammonia Formation and its Removal. Proteolytic Enzymes and Parodontitis. (Stoklasová 3)
Metabolism of Porphyrins and Bile Pigments. Biosynthesis of Haeme and Haemoglobin and their Catabolism. (Kohlerová 1)
Metabolism of Muscles. (Kohlerová 3)
Metabolism of Connective Tissue. Connective Tissue Proteins - Collagen and Elastin. Lack of Vitamin C. Periodontal Ligamentum. Proteoglycans. (Mrkvicová 3)
Blood clotting. (Stoklasová 1)
Calcified Tissues - Composition, Calcification of Bones, Dentine, Enamel, Cementum. Process of Mineralization. Hormones in Mineralization. Dental Plaque and its Metabolism. (Stoklasová 3)
Regulation of Metabolism. (Stoklasová 2)
Laboratory classes & seminars
Lecturers
Prof. Martina Řezáčová, M.D., Ph.D.
Assoc. Prof. Ïng. Milos Hroch, Ph.D.
Assoc. Prof. Jaroslav Cerman, M.D., Ph.D.
Assoc. Prof. Alena Stoklasová, M.D., Ph.D.
Dr. Ivana Brabcová, Ph.D.
Dr. Radim Havelek, Ph.D.
Dr. Renata Kohlerová, Ph.D.
Dr. Alena Mrkvicová, Ph.D.
Dr. Darina Muthná, Ph.D.
Dr. Darja Koutová
Dr. Lucie Melounková
Seminars
Glycosaminoglycans, Glycoproteins. lipids, Terpenes, Structure, Importance. Waxes in Dentistry.
Metabolism of Lipids - Digestion, Absorption, Transport. Metabolism of Fatty Acids, Triacylglycerols, Eicosanoids and Complex Lipids. Metabolism of Lipoproteins. Test.
Metabolism of Steroids and Eicosanoids. Ketogenesis. Test.
Metabolism of Proteins- Digestion, Absorption, Transport. Enzymes involved in degradation of Proteins. Degradation of Ammonia. Biosynthesis of Urea.
Metabolism of Amino Acids. Test.
Biochemistry of Contractile and Connective Tissues. Proteoglycans. Test.
Metabolism of Porphyrins and Bile Pigments.The Blood, Blood Clotting.
Acid-Base Balance. Saliva, Gingival Juice.
Bones and Teeth and their Physical and Chemical Characteristics. Process of Mineralization. Importance in Dentistry. Test.
Metabolism of Water and Ions, Homonal Control. Pulp, Periodontal Ligamentum, Plaque and its Metabolism. Test.
Receptors. Extracellular and Intracellular Communication. Pituitary and Hypothalamic Hormones.
Steroid Hormones, Thyroid Hormones, Hormones of the Pancreas and the Adrenals.
Relationship in Metabolism of Nutrients and its Regulation.
Metabolism of Xenobiotics. Immunochemistry.
Revision Seminar. Metabolism of Teeth and Bones.
Laboratory classes
Concentration of Glucose in the Serum.
Protein Concentration in the Serum. Separation of the Serum Proteins by Electrophoresis.
Concentrations of Urea in the Serum. Concentration of Creatinine in the Serum and Urine.
Concentrations of Calcium and Phosphates in the Serum. Catalytic concentration of Alkaline Phosphatase in the Serum.
Total and Conjugated Bilirubins in the Serum.
Notes: Attendance at laboratory classes and seminars is COMPULSORY and a register is kept. For unavoidable absences (e. g. illness) a written note must be given to doc. Stoklasová immediately on your return.
Literature
Last update: doc. MUDr. Alena Stoklasová, CSc. (22.01.2020)
Compulsory literature
R. Y. Ouellette Introduction to General, Organic and Biological Chemistry. 3rd ed., Mac Millan Publ.Company, New York, 1992. ISBN 0-02-390245-0 (or the latest edition available)
R. K. Murray et al. Harper's Biochemistry. 24th ed., Appleton and Lange, Stamford, USA, 1996.ISBN 0-8385-3612-3. (or the latest edition available)
Recommend literature
P. C. Champe and R.A.Harvey Biochemistry. Lippincott Co, 1987. ISBN 0-397-50801-8 (or the latest edition available)
J.W. Baynes et M. H. Dominiczak Medical Biochemistry. 4th ed., Elsevier 2014. ISBN 978-1-4557-4580-7
