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The non-invasive prenatal diagnosis course gives applicants informations about new possibilities how to obtain genetic informations about fetus during pregnancy from maternal periferal blood. These informations could be determined thanks to the presence of fetal nucleic acids (DNA and RNA) in maternal circulation during pregnancy. Fetal nucleic acids are present in apoptotic bodies and exosomes derived from placental trophoblasts, which are released to maternal circulation during the remodelation of placenta as a consequence of appoptosis and active transport.
Last update: Maternová Kateřina, Bc. (11.01.2023)
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The aim of this course is to inform students about procedures and methods used in anew expansionary discipline of prenatal diagnosis, which obtain the informations about fetus during pregnancy by the analysis of the fetal DNA and RNA present in mothers periferal blood. Last update: Maternová Kateřina, Bc. (11.01.2023)
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Hahn Sinuhe, Jackson Laird G: Prenatal Diagnosis (Methods in Molecular Biology); Humana Press 2008 Pijnenborg Robert, Brosens Ivo, Romero Roberto: Placental Bed Disorders; Cambridge University Press, 2010 Last update: Maternová Kateřina, Bc. (11.01.2023)
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lecture + practical demonstration of laboratory diagnosis Last update: Maternová Kateřina, Bc. (11.01.2023)
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75% point achievement (i.e. minimally 15 points out of 20 maximal) in credit test. Last update: Maternová Kateřina, Bc. (11.01.2023)
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Prenatal diagnosis - non-invasive methods. Molecular biology methods in prenatal diagnosis. 1. Introduction to prenatal diagnosis (invasive and non-invasive, inclusive of non-invasive diagnosis based on fetal microchimerism phenomenon) 2. Importance of fetal sex determination (indications, invasive and non-invasive methods, management of pregnancies at risk of X-linked disorders and congenital adrenal hyperplasia in fetus) 3. Importance of fetal blood group genotyping (aloimmunisation, fetal erythroblastosis, hemolytic disease of newborn, management of pregnancies at risk, invasive and non-invasive methods) 4. Pathologic placentation (pathogenesis, gestational hypertension, preeclampsia, fetal growth restriction, invasive placentation, management of pregnancies at risk, non-invasive methods) 5. Chromosomal aneuploidies, autosomal dominant and autosomal recesive disorders - invasive and non-invasive methods in routine praxis 6. Non-invasive prenatal diagnosis - comprehensive methodology Last update: Maternová Kateřina, Bc. (11.01.2023)
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