Study of the variations in the expression of different adhesion and cytoskeletal proteins of podocytes (E-Cadherin, Podocin, Vimentin) due to Bisphenol A
| Název práce v češtině: | Studie změn v expresi různých adhezivních a cytoskeletálních proteinů podocytů (E-kadherin, Podocin, Vimentin) v důsledku Bisfenolu A |
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| Název v anglickém jazyce: | Study of the variations in the expression of different adhesion and cytoskeletal proteins of podocytes (E-Cadherin, Podocin, Vimentin) due to Bisphenol A |
| Klíčová slova: | bisfenol A, ledviny, podocyt, E-cadherin, podocin, vimentin, exprese proteinů, diabetes mellitus, diabetická nefropatie |
| Klíčová slova anglicky: | bisphenol A, kidney, podocyte, E-cadherin, podocin, vimentin, protein expression, diabetes mellitus, diabetic nephropathy |
| Akademický rok vypsání: | 2017/2018 |
| Typ práce: | diplomová práce |
| Jazyk práce: | angličtina |
| Ústav: | Katedra biologických a lékařských věd (16-16150) |
| Vedoucí / školitel: | PharmDr. Miroslav Kovařík, Ph.D. |
| Řešitel: | skrytý - zadáno vedoucím/školitelem |
| Datum přihlášení: | 10.05.2019 |
| Datum zadání: | 19.08.2019 |
| Datum a čas obhajoby: | 10.09.2019 08:30 |
| Datum odevzdání elektronické podoby: | 23.08.2019 |
| Datum proběhlé obhajoby: | 10.09.2019 |
| Oponenti: | RNDr. Ivana Němečková, Ph.D. |
| Zásady pro vypracování |
| From previous proteomic studies of the R. Bosch's laboratory group, bisphenol A (BPA) has been found to affect some proteins associated with cytoskeleton and cell adhesion. These studies were carried out on animal models of mice CD1. In this work we focuse on selected proteins and want to find out and check changes in their expression both on in vivo and in vitro models. Objectives of this work: · Review literature about Bisphenol A, E-Cadherin, Podocin, Vimentin - all associated with kidneys · Practice the methods: Western Blot, Immunocytochemistry and Immunohistochemistry · Detect the effect of BPA exposure on E-Cadherin, Podocin, Vimentin · Compare the results of in vivo (mice kidneys) and in vitro models (human podocytes) |
| Seznam odborné literatury |
| BOSCH, Ricardo José, Borja QUIROGA, Carmen MUÑOZ-MORENO, Nuria OLEA-HERRERO, María Isabel ARENAS, Marta GONZÁLEZ-SANTANDER, Paula REVENTÚN, Carlos ZARAGOZA, Gabriel DE ARRIBA and Marta SAURA, 2016. El bisfenol A: un factor ambiental implicado en el daño nefrovascular. Nefrologia[online].36(1), 5–9. ISSN 20132514. Available at: doi:10.1016/j.nefroe.2016.01.009 OLEA-HERRERO, Nuria, María Isabel ARENAS, Carmen MUÑÓZ-MORENO, Rafael MORENO-GÓMEZ-TOLEDANO, Marta GONZÁLEZ-SANTANDER, Ignacio ARRIBAS and Ricardo J. BOSCH, 2014. Bisphenol-A induces podocytopathy with proteinuria in mice. Journal of Cellular Physiology[online]. 229(12), 2057–2066. ISSN 10974652. Available at: doi:10.1002/jcp.24665 BOSE, Madhura, Sadia ALMAS and Sharma PRABHAKAR, 2017. Wnt signaling and podocyte dysfunction in diabetic nephropathy [online]. 1–9. Available at: doi:10.1136/jim-2017-000456 PUNTHAKEE, Zubin, Ronald GOLDENBERG and Pamela KATZ, 2018. Definition, Classification and Diagnosis of Diabetes, Prediabetes and Metabolic Syndrome. Canadian Journal of Diabetes[online]. B.m.: Elsevier Inc., 42, S10–S15. ISSN 23523840. Available at: doi:10.1016/j.jcjd.2017.10.003 NAKATSUE, Takeshi, Hiroko KOIKE, Gi Dong HAN, Koichi SUZUKI, Naoko MIYAUCHI, Huaiping YUAN, David J. SALANT, Fumitake GEJYO, Fujio SHIMIZU and Hiroshi KAWACHI, 2005. Nephrin and podocin dissociate at the onset of proteinuria in experimental membranous nephropathy. Kidney International[online]. 67(6), 2239–2253. ISSN 00852538. Available at: doi:10.1111/j.1523-1755.2005.00328.x VAN ROY, F. and G. BERX, 2008. The cell-cell adhesion molecule E-cadherin. Cellular and Molecular Life Sciences[online]. 65(23), 3756–3788. ISSN 1420682X. Available at: doi:10.1007/s00018-008-8281-1 BUCHMAIER, Bettina S., Asima BIBI, Gerhard A. MÜLLER, Gry H. DIHAZI, Marwa ELTOWEISSY, Jenny KRUEGEL and Hassan DIHAZI, 2013. Renal Cells Express Different Forms of Vimentin: The Independent Expression Alteration of these Forms is Important in Cell Resistance to Osmotic Stress and Apoptosis. PLoS ONE[online]. 8(7), 1–13. ISSN 19326203. Available at: doi:10.1371/journal.pone.0068301 Použití další odborné literatury a odborných databází (např. Scopus, Pubmed, Web of Science nebo ScienceDirect) - minimálně 30 odborných citací. |