ATP is an essential biomolecule, which, canonically, is thought of as an energy source for biological reactions or as a building block for the synthesis of nucleic acids. ATP decrease is related to ageing and neurodegeneration. Previous studies found that ATP binds to the microtubule surface, but the role of microtubule-associated ATP is unknown. Tau is axon-specific microtubule-associated protein, which is a critical regulator of microtubule stability and its malfunctioning is associated with defects in mitochondrial transport and neurodegeneration. Syntaphilin is a microtubule-associated protein, which functions as an anchor for mitochondria and ensures proper distribution of mitochondria in axons. Our preliminary experiments suggest that microtubule-associated ATP might affect the interactions of tau and syntaphilin with the microtubule lattice. In this project, the student will in vitro reconstitute tau-microtubule and syntaphilin-microtubule interaction, investigate the cross-talk between tau and syntaphilin and explore the regulation of these proteins by microtubule-associated ATP.
Předběžná náplň práce v anglickém jazyce
ATP is an essential biomolecule, which, canonically, is thought of as an energy source for biological reactions or as a building block for the synthesis of nucleic acids. ATP decrease is related to ageing and neurodegeneration. Previous studies found that ATP binds to the microtubule surface, but the role of microtubule-associated ATP is unknown. Tau is axon-specific microtubule-associated protein, which is a critical regulator of microtubule stability and its malfunctioning is associated with defects in mitochondrial transport and neurodegeneration. Syntaphilin is a microtubule-associated protein, which functions as an anchor for mitochondria and ensures proper distribution of mitochondria in axons. Our preliminary experiments suggest that microtubule-associated ATP might affect the interactions of tau and syntaphilin with the microtubule lattice. In this project, the student will in vitro reconstitute tau-microtubule and syntaphilin-microtubule interaction, investigate the cross-talk between tau and syntaphilin and explore the regulation of these proteins by microtubule-associated ATP.
- zadáno a potvrzeno stud. odd.