Lipidomická analýza tukové tkáně pacientů s ischemickou chorobou srdeční

• Název práce v češtině: Lipidomická analýza tukové tkáně pacientů s ischemickou chorobou srdeční
• Název v anglickém jazyce: Lipidomic analysis of adipose tissue in patients with ischemic heart disease
• Klíčová slova: Lipidomická analýza, epikardiální tuková tkáň, subkutánní tuková tkáň, ischemická choroba srdeční, diabetes mellitus 2. typu
• Klíčová slova anglicky: Lipidomic analysis, epicardial adipose tissue, subcutaneous adipose tissue, coronary artery disease, type 2 diabetes mellitus
• Akademický rok vypsání: 2021/2022
• Typ práce: disertační práce
• Jazyk práce: čeština
• Ústav: IV. interní klinika – gastroenterologie a hepatologie 1. LF UK a VFN (11-00540)
• Vedoucí / školitel: RNDr. Marek Vecka, Ph.D.
• Řešitel: skrytý - zadáno a potvrzeno stud. odd.
• Datum přihlášení: 14.12.2021
• Datum zadání: 14.12.2021
• Datum potvrzení stud. oddělením: 14.12.2021
• Datum a čas obhajoby: 22.06.2022 13:00
• Místo konání obhajoby: posluchárna Ústavu lékařské biochemie a laboratorní diagnostiky 1. LF UK a VFN
• Datum odevzdání elektronické podoby: 10.04.2022
• Datum proběhlé obhajoby: 22.06.2022
• Předmět: Obhajoba dizertační práce (B90002)
• Oponenti: prof. Ing. Rudolf Poledne, CSc.

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Předběžná náplň práce

Ischemická choroba srdeční je celosvětově jednou z nejčastějších příčin úmrtí. Vzniká v důsledku ucpávání cév aterogenními pláty. Rozvoj onemocnění, její prognóza a léčba je ovlivněna častou přítomností dalších komorbidit, jako jsou diabetes mullitu 2. typu či obezita. Všechna tato onemocnění souvisejí s metabolismem lipidů a s tukovou tkání. Tuková tkáň již není považována za pouhé úložiště energie, ale je důležitým endokrinním orgánem, jejíž působky mají dopad na celotělový metabolismus. Hlavním cílem práce bude zavést metodu vhodnou pro analýzu lipidů v tukové tkáni, zhodnotit vliv umístění tukové tkáně v podkoží a v epikardu na profil lipidů a dále posoudit vliv ischemické choroby na jejich složení. K dosažení cílů v práci bude použito pokročilých analytických metod, jako je vysokoúčinná kapalinová chromatografie a hmotnostní spektrometrie v rámci necílené lipidomiky, což umožní sledovat a zhodnotit vliv širokého profilu látek.