RNAi is an ancestral antiviral innate immunity pathway abandoned during vertebrate evolution. However, it is possible to boost its function by modification of one of the enzymes in the pathway. This project will capitalize on mouse mutants, which carry a genetic modification enhancing RNAi. It will examine of effects of augmented RNAi in vivo. Using genetically modified mouse models, the aim is to a) examine genetically-induced RNAi pathway activity in different tissues and cell types, b) assess detrimental effects of RNAi in cells, organs and the whole mouse, and c) analyze competition, cooperativity & redundancy between RNAi and other dsRNA-responding pathways.
Předběžná náplň práce v anglickém jazyce
RNAi is an ancestral antiviral innate immunity pathway abandoned during vertebrate evolution. However, it is possible to boost its function by modification of one of the enzymes in the pathway. This project will capitalize on mouse mutants, which carry a genetic modification enhancing RNAi. It will examine of effects of augmented RNAi in vivo. Using genetically modified mouse models, the aim is to a) examine genetically-induced RNAi pathway activity in different tissues and cell types, b) assess detrimental effects of RNAi in cells, organs and the whole mouse, and c) analyze competition, cooperativity & redundancy between RNAi and other dsRNA-responding pathways.
- zadáno vedoucím/školitelem