Populační dynamika a speciace u vybraných rodů mechových dřepčíků (Coleoptera: Chrysomelidae: Alticini)

• Název práce v češtině: Populační dynamika a speciace u vybraných rodů mechových dřepčíků (Coleoptera: Chrysomelidae: Alticini)
• Název v anglickém jazyce: Population dynamics and speciation in selected genera of moss-inhabiting flea beetles (Coleoptera: Chrysomelidae: Alticini)
• Akademický rok vypsání: 2019/2020
• Typ práce: disertační práce
• Jazyk práce: čeština
• Ústav: Katedra zoologie (31-170)
• Vedoucí / školitel: doc. Mgr. Pavel Munclinger, Ph.D.
• Řešitel: skrytý - zadáno vedoucím/školitelem
• Datum přihlášení: 10.10.2019
• Datum zadání: 10.10.2019
• Konzultanti: Mgr. Zuzana Musilová, Ph.D.

Zásady pro vypracování

UNIX a práce s genomickými daty - MB170C47
Evoluční genetika - MB170P102

Předběžná náplň práce

The main aims of the PhD projects are: (1) uncovering possible gene flows between isolated populations of flightless moss-inhabiting flea beetles; (2) comparison of gene flow in mitochondrial and nuclear genes between isolated montane populations of completely flightless beetles and Clavicornaltica species with winged males; (3) comparison of population dynamics of flightless moss-inhabiting flea beetles from sky islands (forming isolated populations) and from low altitudes (forming connected ranges); (4) study the possibility of jumping ability being an advantage for aerial dispersal in flea beetles.
The mentioned goals of this project are planned to be achieved by the following research plan: (1) we will collect samples of various populations of moss-inhabiting flea beetles (at least 15 specimens per locality on at least 5 different moutain sites; as collecting sites will be selected mountain habitats presumed to have isolated populations); (2) we will use ready sequenced moss-inhabiting flea beetle genomes and the NGS RADsequencing approach for analysis of single-nucleotide polymorphisms between members of various isolated populations for uncovering recent or ancient gene flows; (3) we will use the same molecular approach to analyse collected material of partially winged Clavicornaltica malayana populations from 3 different mountains in Malaysia and compare the value of partial flight ability for gene flow between isolated montane populations in flea beetles, as well as the differences between gene flow in mitochondrial and nuclear genes between studied populations; (4) we will perform experiments for testing jumping ability as an advantage to get into the aerial flow and disperse easily. The dispersal experiments will be conducted both in the lab (experimental exposition of moss-inhabiting flea beetles and non-jumping same-sized arthropod sample contro to windflow inside an aerial tunnel), and on-site (setting Malaise traps and flight intercept traps to habitats of moss-inhabiting flea beetles for revealing their presence or absence in the aerial plankton).
The field collections will be conducted on material of Ivalia species from sky islands (I. uenoi, I. bella) and from low elevations (I. sp. nov.) distributed in Taiwan. Part of the material necessary for the study is allready in hand. Population samples of Clavicornaltica malayana, which are allready in hand and ready for analysis, were collected in Cameron Highlands, Malaysia. The reference genomes of Ivalia and Clavicornaltica will be allready in hand. International research collaboration with Taiwanese colleagues and institutions is allready established and collection permits will be available for the research. No samples, which are planned to be used in the study, are in subject of the Nagoya protocol.

Předběžná náplň práce v anglickém jazyce

The main aims of the PhD projects are: (1) uncovering possible gene flows between isolated populations of flightless moss-inhabiting flea beetles; (2) comparison of gene flow in mitochondrial and nuclear genes between isolated montane populations of completely flightless beetles and Clavicornaltica species with winged males; (3) comparison of population dynamics of flightless moss-inhabiting flea beetles from sky islands (forming isolated populations) and from low altitudes (forming connected ranges); (4) study the possibility of jumping ability being an advantage for aerial dispersal in flea beetles.
The mentioned goals of this project are planned to be achieved by the following research plan: (1) we will collect samples of various populations of moss-inhabiting flea beetles (at least 15 specimens per locality on at least 5 different moutain sites; as collecting sites will be selected mountain habitats presumed to have isolated populations); (2) we will use ready sequenced moss-inhabiting flea beetle genomes and the NGS RADsequencing approach for analysis of single-nucleotide polymorphisms between members of various isolated populations for uncovering recent or ancient gene flows; (3) we will use the same molecular approach to analyse collected material of partially winged Clavicornaltica malayana populations from 3 different mountains in Malaysia and compare the value of partial flight ability for gene flow between isolated montane populations in flea beetles, as well as the differences between gene flow in mitochondrial and nuclear genes between studied populations; (4) we will perform experiments for testing jumping ability as an advantage to get into the aerial flow and disperse easily. The dispersal experiments will be conducted both in the lab (experimental exposition of moss-inhabiting flea beetles and non-jumping same-sized arthropod sample contro to windflow inside an aerial tunnel), and on-site (setting Malaise traps and flight intercept traps to habitats of moss-inhabiting flea beetles for revealing their presence or absence in the aerial plankton).
The field collections will be conducted on material of Ivalia species from sky islands (I. uenoi, I. bella) and from low elevations (I. sp. nov.) distributed in Taiwan. Part of the material necessary for the study is allready in hand. Population samples of Clavicornaltica malayana, which are allready in hand and ready for analysis, were collected in Cameron Highlands, Malaysia. The reference genomes of Ivalia and Clavicornaltica will be allready in hand. International research collaboration with Taiwanese colleagues and institutions is allready established and collection permits will be available for the research. No samples, which are planned to be used in the study, are in subject of the Nagoya protocol.