Impact of PPM1D/Wip1 phosphatase on chromatin organization in human cells
| Název práce v češtině: | Vliv PPM1D/Wip1 fosfatázy na organizaci chromatinu v lidských buňkách |
|---|---|
| Název v anglickém jazyce: | Impact of PPM1D/Wip1 phosphatase on chromatin organization in human cells |
| Akademický rok vypsání: | 2019/2020 |
| Typ práce: | disertační práce |
| Jazyk práce: | angličtina |
| Ústav: | Katedra buněčné biologie (31-151) |
| Vedoucí / školitel: | MUDr. Libor Macůrek, Ph.D. |
| Řešitel: | skrytý - zadáno vedoucím/školitelem |
| Datum přihlášení: | 18.10.2019 |
| Datum zadání: | 18.10.2019 |
| Seznam odborné literatury |
| Jaiswal et al., ATM/Wip1 activities at chromatin control Plk1 re-activation to determine G2 checkpoint duration. EMBO J. 2017; 36:2161-2176.Kleiblova et al., Gain-of-function mutations of PPM1D/Wip1 impair the p53-dependent G1 checkpoint. J Cell Biol. 2013; 201:511-21.Macůrek et al., Wip1 phosphatase is associated with chromatin and dephosphorylates gammaH2AX to promote checkpoint inhibition. Oncogene. 2010; 29:2281-91. |
| Předběžná náplň práce |
| Genome instability caused by DNA repair can contribute to various pathologies including cancer. Genome integrity is protected by coordinated action of molecular pathways that control cell cycle and DNA repair. The Pan-Nuclear Level.This project aims to study changes in the chromatin organization caused by genotoxic stress. Protein phosphatase PPM1D / Wip1 is localized to chromatin and inactivates cellular response to DNA damage.Here we will address the impact of PPM1D / Wip1 phosphatase on chromatin organization in human cells.In particular, we will compare the distribution of heterochromatin in control cells PPM1D / Wip1 was inactivated by CRISPR / Cas9 technology and cells treated with a small molecule inhibitor of PPM1D / Wip1.Chromatin organization will be studied in normal conditions and after induction of DNA damage using various molecular and cell biology techniques.We expect this project to contribute to better understanding of cellular responses to DNA damage. We are looking for a motivated PhD student to join our young international team located in Prague, Czech Republic. |
| Předběžná náplň práce v anglickém jazyce |
| Genome instability caused by DNA repair can contribute to various pathologies including cancer. Genome integrity is protected by coordinated action of molecular pathways that control cell cycle and DNA repair. The Pan-Nuclear Level.This project aims to study changes in the chromatin organization caused by genotoxic stress. Protein phosphatase PPM1D / Wip1 is localized to chromatin and inactivates cellular response to DNA damage.Here we will address the impact of PPM1D / Wip1 phosphatase on chromatin organization in human cells.In particular, we will compare the distribution of heterochromatin in control cells PPM1D / Wip1 was inactivated by CRISPR / Cas9 technology and cells treated with a small molecule inhibitor of PPM1D / Wip1.Chromatin organization will be studied in normal conditions and after induction of DNA damage using various molecular and cell biology techniques.We expect this project to contribute to better understanding of cellular responses to DNA damage. We are looking for a motivated PhD student to join our young international team located in Prague, Czech Republic. |