Nuclear bodies are dynamic micron-scale organelles that lack lipid bilayers. They are involved in essential aspects of cellular metabolism and have been implicated in human diseases, such as ALS and SMA. However, the basic principles that drive their formation and function are poorly understood. We plan to utilized super-resolution light microscopic methods (SIM, STED) and electron microscopy to study the arrangement of protein and RNA nuclear body components in fixed or live samples (in case of light microscopy). We will combine fluorescent RNA detection with imaging of endogenous levels of GFP-tagged proteins and plan to adapt FRAP methods to super-resolution light microscopy to obtain kinetic parameters that will be used to model nuclear body assembly, maintenance and function.
- zadáno vedoucím/školitelem