Functional specialization of EXO70A and EXO70B paralogs of the EXO70 exocyst subunit in Arabidopsis.
| Název práce v češtině: | Funkční specializace paralogů EXO70A a EXO70B podjednotky exocystu EXO70 u Arabidopsis |
|---|---|
| Název v anglickém jazyce: | Functional specialization of EXO70A and EXO70B paralogs of the EXO70 exocyst subunit in Arabidopsis. |
| Klíčová slova: | exocyst; secretion; exocytosis; autophagy; EXO70; |
| Klíčová slova anglicky: | exocyst; secretion; exocytosis; autophagy; EXO70; |
| Akademický rok vypsání: | 2015/2016 |
| Typ práce: | disertační práce |
| Jazyk práce: | angličtina |
| Ústav: | Katedra experimentální biologie rostlin (31-130) |
| Vedoucí / školitel: | prof. RNDr. Viktor Žárský, CSc. |
| Řešitel: | skrytý - zadáno vedoucím/školitelem |
| Datum přihlášení: | 22.10.2015 |
| Datum zadání: | 22.10.2015 |
| Datum odevzdání elektronické podoby: | 10.05.2021 |
| Datum proběhlé obhajoby: | 06.08.2021 |
| Oponenti: | prof. Shaul Yalovsky |
| prof. doc. Mgr. Miroslav Ovečka, Ph.D. | |
| Konzultanti: | Mgr. Ivan Kulich, Ph.D. |
| Mgr. Lukáš Synek, Ph.D. | |
| prof. RNDr. Fatima Cvrčková, Dr. | |
| Ocenění: | Práce byla navržena na ocenění |
| Předběžná náplň práce |
| The exocyst is a complex of eight proteins mediating first contact (tethering) between secretory vesicles and the target membrane. It was discovered in yeast as an effector of RAB and RHO small GTPases and we found it to function also in land plants (Hála et al. 2008). Exocyst is implied in regulation of cell polarity and morphogenesis including cytokinesis, recycling of PM proteins (incl. PINs), cell wall biogenesis, fertilization, stress responses and biotic interactions including defense against pathogens (Žárský et al. 2013).
Working on exocytotic functions of the plant exocyst - a vesicle tethering complex - we unexpectedly found a version of exocyst complex dominated by EXO70B1 subunit which is involved in an autophagy related membrane and secondary metabolites traffic to the vacuole - in a pathway not involving Golgi apparatus (Kulich et al. 2013). This STARS project proposal aims to further clarify autophagy related exocyst functions in plants - in which stages of autophagy it is involved starting with autophagophore initiation till the vacuole delivery and which partner proteins are interacting. The PhD student will study probable competition of EXO70 paralogs for exocyst core subunits and resulting changes in vesicle transport targeting. |
| Předběžná náplň práce v anglickém jazyce |
| The exocyst is a complex of eight proteins mediating first contact (tethering) between secretory vesicles and the target membrane. It was discovered in yeast as an effector of RAB and RHO small GTPases and we found it to function also in land plants (Hála et al. 2008). Exocyst is implied in regulation of cell polarity and morphogenesis including cytokinesis, recycling of PM proteins (incl. PINs), cell wall biogenesis, fertilization, stress responses and biotic interactions including defense against pathogens (Žárský et al. 2013).
Working on exocytotic functions of the plant exocyst - a vesicle tethering complex - we unexpectedly found a version of exocyst complex dominated by EXO70B1 subunit which is involved in an autophagy related membrane and secondary metabolites traffic to the vacuole - in a pathway not involving Golgi apparatus (Kulich et al. 2013). This STARS project proposal aims to further clarify autophagy related exocyst functions in plants - in which stages of autophagy it is involved starting with autophagophore initiation till the vacuole delivery and which partner proteins are interacting. The PhD student will study probable competition of EXO70 paralogs for exocyst core subunits and resulting changes in vesicle transport targeting. |