Small RNA molecules regulating gene expression received a status of omnipresent master regulators of eukaryotic lives with almost supernatural powers. Mammals hold at least three mechanisms employing small RNA molecules for regulating gene expression. One of these mechanisms, the microRNA (miRNA) pathway, involves currently over a thousand of genome-encoded different miRNAs that are claimed to extend their control over more than a half of a genome. However, mouse oocytes and early embryos ignore the regulatory power of miRNAs, adding another surprising feature to the field of small RNAs. The aim of this thesis work is to test one of the potential mechanisms inhibiting the miRNA function in mouse oocytes: short Argonaute isoforms whose expression was discovered in mouse oocytes.
Předběžná náplň práce v anglickém jazyce
Small RNA molecules regulating gene expression received a status of omnipresent master regulators of eukaryotic lives with almost supernatural powers. Mammals hold at least three mechanisms employing small RNA molecules for regulating gene expression. One of these mechanisms, the microRNA (miRNA) pathway, involves currently over a thousand of genome-encoded different miRNAs that are claimed to extend their control over more than a half of a genome. However, mouse oocytes and early embryos ignore the regulatory power of miRNAs, adding another surprising feature to the field of small RNAs. The aim of this thesis work is to test one of the potential mechanisms inhibiting the miRNA function in mouse oocytes: short Argonaute isoforms whose expression was discovered in mouse oocytes.
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