Hlavni naplni prace je vyvoj a synteza novych dopravnich systemu na bazi syntetickych hydrofilnich polymeru urcenych pro cileny prenos a rizene uvolnovani siRNA. Duraz bude kladen jak na pripravu polymernich nosicu zajistujicich bezpecnou dopravu a efektivni uvolnovani siRNA v cilovych bunkach, tak na pochopeni vztahu mezi fyzikalne-chemickymi vlastnostmi a biologickym chovanim vyslednych polymer/siRNA konjugatu v in vitro podminkach. V praci budou vyuzity multidisciplinarni metodiky zahrnujici moderni synteticke metody jako jsou RAFT polymerace nebo click chemie, sofistikovane fyzikalni techniky zalozene na mereni statickeho a dynamickeho rozptylu svetla ci molekularne-biologicke postupy jako jsou prace s bunecnymi kulturami nebo mereni genove exprese.
Předběžná náplň práce v anglickém jazyce
The main objective of the work is development and synthesis of the hydrophilic polymers-based delivery systems intended for the targeted delivery and controlled release of siRNA. Particular attention will be paid to the preparation of polymer carriers providing safe transport and effective release of siRNA in the target cells as well as the comprehension of relation between the physicochemical properties and biological behaviour of the resulting polymer/siRNA conjugates in vitro. Multidisciplinary methods including modern synthetic procedures (i.e. RAFT polymerization and click chemistry), sophisticated physical techniques based on static and dynamic light scattering or molecular biology procedures (i.e. cell culture and gene expression measurement) will be applied in this work.
- zadáno vedoucím/školitelem