Molecular mechanisms involved in genotoxicity of industrially important monomers (styrene, 1,3-butadiene)
Molecular mechanisms involved in genotoxicity of industrially important monomers (styrene, 1,3-butadiene)
dizertační práce (OBHÁJENO)
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Trvalý odkaz
http://hdl.handle.net/20.500.11956/93741Identifikátory
SIS: 77243
Kolekce
- Kvalifikační práce [19109]
Autor
Vedoucí práce
Oponent práce
Černá, Marie
Machala, Miroslav
Fakulta / součást
Přírodovědecká fakulta
Obor
Molekulární a buněčná biologie
Katedra / ústav / klinika
Katedra genetiky a mikrobiologie
Datum obhajoby
15. 9. 2009
Nakladatel
Univerzita Karlova, Přírodovědecká fakultaJazyk
Angličtina
Známka
Prospěl/a
2 Contents ABSTRACT........................................................................................................... 2 1. INTRODUCTION............................................................................................. 4 2. AIMS OF THE PROJECT:.............................................................................. 8 2. MATERIAL AND METHODS:....................................................................... 9 3. RESULTS: ....................................................................................................... 10 4. CONCLUSIONS.............................................................................................. 16 5. REFERENCES................................................................................................ 18 INDEX OF PUBLICATIONS:........................................................................... 21 Publications in extenso, which are the base of PhD. thesis .............................. 21 Publications in extenso, without relationship to the PhD thesis...................... 22 ABSTRACT The evaluation of individual health risk in workers occupationally exposed to industrial xenobiotics requires the use of a large number of parameters reflecting external exposure, internal exposure, biological effects and individual susceptibility....
1 ABSTRACT The evaluation of individual health risk in workers occupationally exposed to industrial xenobiotics requires the use of a large number of parameters reflecting external exposure, internal exposure, biological effects and individual susceptibility. Environmental, occupational and life style-related exposure to mutagenic agents may contribute to cancer risk in humans. To prevent the potentially hazardous effects of such agents it is important to understand their mechanisms of action. Styrene is one of the most important monomer for producing polymers and copolymers in plastics, latex paints and together with 1,3-butadiene (BD) in the manufacture of synthetic rubbers. In this thesis, a large set of parameters, including markers of external and internal exposure and biomarkers of biological effects and susceptibility have been studied in relation to the occupational exposure to both styrene and BD. First part of the present study was focused on evaluating the role of various biomarkers to assess genotoxic effects of above mentioned xenobiotics. Biomarkers reflecting styrene- and BD-induced genotoxicity and mutagenicity: O6 -styrene guanine DNA adducts, haemoglobin adducts, single-strand breaks (SSBs), SSB Endo III sites, chromosomal aberrations (CA), hypoxanthine-guanine phosphoribosyltransferase...