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Výsledky projektu Počáteční fáze formování sestřihového aparátu

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(Celkem 1 zázn.)
Stejskalová, Eva. The splicing factor U1-70K interacts with the SMN complex and is required for nuclear gem integrity. Journal of Cell Science, 2014, sv. 15, s. 3909–3915. ISSN 1477-9137. IF 5.325. [Článek v časopise]
The nuclear SMN complex localizes to specific structures called nuclear gems. The loss of gems is a cellular marker for several neurodegenerative diseases. Here, we identify that the U1-snRNP-specific protein U1-70K localizes to nuclear gems, and we show that U1-70K is necessary for gem integrity. Furthermore, we show that the interaction between U1-70K and the SMN complex is RNA independent, and we map the SMN complex binding site to the unstructured N-terminal tail of U1-70K. Consistent with these results, the expression of the U1-70K N-terminal tail rescues gem formation. These findings show that U1-70K is an SMN-complex-associating protein, and they suggest a new function for U1-70K in the formation of nuclear gems.