Poslední úprava: Mgr. Lenka Doubravská, Ph.D. (15.03.2019)
Designed for those interested in histological methodologies, suitable as a supplement lectures on histology. Recommended to take the course first year of bc. study after finishing the Practicals of Cell Biology. This is a basic course of histological techniques, where students learn about preparing smearing, stemping and cutting types of histological specimens, including fluorescent variant of staining.
Literatura - angličtina
Poslední úprava: Mgr. Lenka Doubravská, Ph.D. (14.03.2019)
For this practicalis not required any special literature, protocols are provided. Atthe beginning ofthe practicalexercisesall issues are thoroughly discussed and allprotocoarer are available.
Požadavky ke zkoušce - angličtina
Poslední úprava: Mgr. Lenka Doubravská, Ph.D. (14.03.2019)
Forgetting the credits the 100%attendance is obligatory. Students have to prepare set of requiredspecimensandtheircapture their images using a microscopeequipped with acolor camerawithhigh resolution- bothin the fluorescencemodeandintransmitted light.
Sylabus - angličtina
Poslední úprava: Mgr. Lenka Doubravská, Ph.D. (14.03.2019)
1. Preparationof histological specimens:the procurement of tissuesand organs (mouse), fixation,drainage using ascendingseries of alcohol, embeddingin paraffin,cuttingona microtome,stainingwith hematoxylin/eosin,closinginCanadian balsam. 2. Preparationof smearing specimensof bloodandbonemarrow:methanol fixation,Giemsa 3. Preparationof stampingspecimens of selected organs- spleen,thymus, lymph nodes, testes,cerebellumfixationwith methanol,Giemsa 4. Preparationof fluorescently labeled specimens(detectionof plasma cellsin the spleen, characterizationof the developmental stages ofspermatogenesisin the testis) 5. Preparation of immunofluorescentslidesfor fluorescencemicroscopy(fixation,permeabilizationblocking,staining withprimary and secondaryantibodies,DAPI,embedinginMowiol)