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Course, academic year 2023/2024
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Cuticular Analysis - OENBB1704Z
Title: Cuticular analysis
Guaranteed by: Katedra biologie a environmentálních studií (41-KBES)
Faculty: Faculty of Education
Actual: from 2020
Semester: both
E-Credits: 4
Hours per week, examination: 0/2, Ex [HT]
Capacity: winter:unknown / unknown (5)
summer:unknown / unknown (5)
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: not taught
Language: English
Teaching methods: full-time
Teaching methods: full-time
Note: course can be enrolled in outside the study plan
enabled for web enrollment
priority enrollment if the course is part of the study plan
you can enroll for the course in winter and in summer semester
Guarantor: doc. RNDr. Vasilis Teodoridis, Ph.D.
Annotation
Last update: Kateřina Esserová, DiS. (11.06.2019)
The subject has a theoretical and practical part. The theoretical part focuses on a definition of the basic principles of the cuticular analysis, history, and shows a basic overview of the leaf anatomical structure, techniques of preparation and methods of leaf maceration based on use of the evergreen and deciduous leaves. Basis of the terminology and topography in epidermal characteristics (i.e., idiocuticular and epicuticular features) including a morphological classification of stomata, trichomes and hypodermic objects are presented. The practical part is focused on a demonstration of the main epidermal features mainly by gymnosperms and angiosperms including their detailed scientific descriptions, practical preparation and determination of the modern and fossil samples.
Literature
Last update: Kateřina Esserová, DiS. (11.06.2019)

Dilcher, D. (1974): Approaches to the identification of angiosperm leaf remains. Botanical Review 40. New York Botanical Garden.

Manual of Leaf Architecture - morphological description and categorization of dicotyledonous and net-veined monocotyledonous angiosperms by Leaf Architecture Working Group. 65p.

Requirements to the exam
Last update: Kateřina Esserová, DiS. (11.06.2019)

Oral examination, knowledge of subject matter within the scope of the syllabus.

Syllabus
Last update: Kateřina Esserová, DiS. (11.06.2019)

Lectures:
1. Definitions and principles of cuticular analysis and its history, types of cuticular analysis (mass analysis, fragments analysis, cuticle analysis in situ), anatomical structure of the leaf, cuticle sensu stricto, sensu lato, preparation techniques, peeling method.

2. Terminology: Idiocentric features - thickness, sculpture, papillae; Epicuticular features - normal epidermal cells (outlines, anticlinal wall), types of trichomes and their bases, types of ventricles and their classification (polocytic, tetracytic, paracytic, anisocytic, diacytic, pericytic, desmocytic, copericytic, amphipericytic), hydatodes, hypodermis, idoblasts and internal secretory organs (scleroids, resin channels, secretion reservoirs); Topography: orientation, distribution and density of individual epicuticular features (stomatal density, stomatal index).

Exercises:
3-9. Practical demonstration and description of cuticular structures in selected groups of gymnosperms and angiosperms.
10. Preparation and preparation of permanent preparations.

 
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