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Last update: VECER/MFF.CUNI.CZ (16.05.2008)
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Last update: VECER/MFF.CUNI.CZ (16.05.2008)
To learn advanced techniques of the time resolved fluorescence spectroscopy - data acquisition and analysis in time domain |
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Last update: T_FUUK (28.04.2008)
Lakowicz J. R. Principles of fluorescence spectroscopy, Second edition. New York: Kluwer Academic/Plenum Publishers, 1999.
Večeř J., Kowalczyk A. A. and Dale R. E., Improved recursive convolution integral for the analysis of fluorescence decay data: Local approximation of the apparatus response function by a general polynomial. Rev.Sci.Instrum. 64 (1993) 3403-3412
Večeř J., Kowalczyk A. A., Davenport L. and Dale R. E., Reconvolution analysis in time-resolved fluorescence experiments - an alternative approach: Reference-to-excitation-to-fluorescence reconvolution. Rev.Sci.Instrum. 64 (1993) 3413-3424
Bevington P. V. and Robinson D. K., Data reduction and error analysis for the physical sciences, Third edition, Mc Graw Hill, 2003
Brochon, J.C. Methods Enzymol. 240 (1994) 262-311 Bryan RK. 1990. Maximum-Entropy Analysis of Oversampled Data Problems. European Biophysics Journal 18 (1990) 165-174.
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Last update: VECER/MFF.CUNI.CZ (16.05.2008)
lecture |
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Last update: T_FUUK (14.02.2006)
Fluorescence lifetime measurements in both time and frequency domain Fluorescence decay curves: basic characteristics; basics of the fluorescence decay analysis Convolution integral and its approximations; the role of the response function in the analysis The use of monoexponential references in the fluorescence decay experiments Response function calculations based on the reference decay measurements Analytical simulations of the fluorescence decay and anisotropy curves Parametric analysis of the fluorescence decay data: potential and limitations The basic principles of the maximum entropy method Time-resolved fluorescence spectroscopy and 3D peptide studies |