Membrane transport, membrane transport proteins. Methods of the study of their structure and function. Channels,
transporters, antiport, symport, active transport. Important families of membrane transport proteins, focus on several
examples. Known mechanisms of the membrane transport.
Last update: T_FUUK (28.04.2008)
Membránový transport, membránové proteiny. Metody studia jejich struktury a funkce. Kanály, transportéry, antiport,
symport, aktivní transport. Význačné rodiny membránových transportních proteinů a blíže rozebrané konkrétní příklady.
Dosud známé mechanismy membránového transportu.
Aim of the course -
Last update: T_FUUK (12.05.2008)
Study of membrane transport, membrane transport proteins and relevant experimental methods.
Last update: T_FUUK (12.05.2008)
Cílem předmětu je prohloubení znalostí o membránovém transportu, membránových transportních proteinech a o metodách jejich studia.
Literature -
Last update: URBANKOV/MFF.CUNI.CZ (11.02.2009)
Books:
Kotyk A., Janáček K. and Koryta J. (1988) Biophysical Chemistry of Membrane Function (Chichester: Wiley)
Yeagle, P.L. (2004) The Structure of Biological Membranes (CRC)
Nicholls, D.G, S. J. (2002) Bioenergetics 3 (Academic press)
Tamm, L.K. (2005) Protein-lipid interactions: From membrane domains to cellular networks (Wiley-Vch Verlag), Weinheim, Germany
White, S.H. (1994) Membrane protein structure: Experimental approaches (Oxford University press)
Van Winkle, L.J. (1995) Biomembrane Transport (Academic press)
Hille,B. (2001) Ion channels of excitable membranes (Sinauer Associtates, Inc.)
Creighton,T.E. (1993) Proteins (W.H. Freeman and company)
B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, P. Walter (2002) Molecular Biology of the Cell (Garland Science)
Articles:
Marsh,D.: Peptide models for membrane channels (1996) Biochem. J. 315: 345-361
Spencer,R.H., Rees,D.C.: The a-helix and the organization and gating of channels (2002) Annu. Rev. Biophys. Biomol. Struct. 31: 207-33
Wimley,W.C.: The versatile b-barrel memrane protein (2003) Curr. Opin. Struct. Biol. 13: 404-411
MacKinnon,R.: Potassium channels (2003) FEBS Lett. 555: 62-65
Abramson,J., Kaback,H.R., Iwata,S.: Structural comparison of lactose permease and the glycerol-3-phosphate antiporter: members of the major facilitator superfamily (2004) Curr.Opin. Struct. Biol. 14: 413-419
Hunte,C., Screpanti,E., Venturi,M., Rimon,A., Padan,E., Michel,H.: Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH (2005) Nature 435: 1197-1202
White,S.H.: Membrane protein folding and stability: physical principles (1999) Annu. Rev. Biophys. Biomol. Struct. 28: 319-65
Arechaga,I., Jones,P.C.: The rotor in the membrane of the ATP synthase and relatives (2001) FEBS Lett. 494: 1-5
Apell,H.J.: Structure-function relationship in P-type ATPases - a biophysical approach (2003) Rev. Physiol. Biochem. Pharmacol 150: 1-35
Schultz,B.E., Chan,S.I.: Structures and proton-pumping strategies of mitochondrial respiratory enzymes (2001) Annu. Rev. Biophys. Biomol. Struct. 30: 23-65
WWW pages:
www.tcdb.org
http://pdb.rcsb.org/
Last update: URBANKOV/MFF.CUNI.CZ (11.02.2009)
Knihy, skripta:
Kotyk A., Janáček K. and Koryta J. (1988) Biophysical Chemistry of Membrane Function (Chichester: Wiley)
Yeagle, P.L. (2004) The Structure of Biological Membranes (CRC)
Nicholls, D.G, S. J. (2002) Bioenergetics 3 (Academic press)
Tamm, L.K. (2005) Protein-lipid interactions: From membrane domains to cellular networks (Wiley-Vch Verlag), Weinheim, Germany
White, S.H. (1994) Membrane protein structure: Experimental approaches (Oxford University press)
Van Winkle, L.J. (1995) Biomembrane Transport (Academic press)
Hille,B. (2001) Ion channels of excitable membranes (Sinauer Associtates, Inc.)
Creighton,T.E. (1993) Proteins (W.H. Freeman and company)
B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, P. Walter (2002) Molecular Biology of the Cell (Garland Science)
Články:
Marsh,D.: Peptide models for membrane channels (1996) Biochem. J. 315: 345-361
Spencer,R.H., Rees,D.C.: The a-helix and the organization and gating of channels (2002) Annu. Rev. Biophys. Biomol. Struct. 31: 207-33
Wimley,W.C.: The versatile b-barrel memrane protein (2003) Curr. Opin. Struct. Biol. 13: 404-411
MacKinnon,R.: Potassium channels (2003) FEBS Lett. 555: 62-65
Abramson,J., Kaback,H.R., Iwata,S.: Structural comparison of lactose permease and the glycerol-3-phosphate antiporter: members of the major facilitator superfamily (2004) Curr.Opin. Struct. Biol. 14: 413-419
Hunte,C., Screpanti,E., Venturi,M., Rimon,A., Padan,E., Michel,H.: Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH (2005) Nature 435: 1197-1202
White,S.H.: Membrane protein folding and stability: physical principles (1999) Annu. Rev. Biophys. Biomol. Struct. 28: 319-65
Arechaga,I., Jones,P.C.: The rotor in the membrane of the ATP synthase and relatives (2001) FEBS Lett. 494: 1-5
Apell,H.J.: Structure-function relationship in P-type ATPases - a biophysical approach (2003) Rev. Physiol. Biochem. Pharmacol 150: 1-35
Schultz,B.E., Chan,S.I.: Structures and proton-pumping strategies of mitochondrial respiratory enzymes (2001) Annu. Rev. Biophys. Biomol. Struct. 30: 23-65
WWW stránky:
www.tcdb.org
http://pdb.rcsb.org/
Teaching methods -
Last update: T_FUUK (12.05.2008)
lecture
Last update: T_FUUK (12.05.2008)
přednáška
Syllabus -
Last update: T_FUUK (28.04.2008)
1. Lipid (bio)membrane. Membrane transport - ions, nonpolar vs. polar molecules, macromolecules. unspecific permeation, active and passive transport. Thermodynamic and kinetic aspects. Membrane potential.
2. Methods of study of membrane transport. Passive transport - unspecific permeation, facilitated diffusion. Channels, transporters. Oligopeptides as a model of membrane proteins.
3. Protein structure (focus on membrane proteins), experimental methods and prediction of protein structure.
4. Theoretical description of transport processes.
8. Transport of ions (P-, V- and F- type ATPases), ABC pumps.
9. Active transport coupled with redox reaction - proteins of respiratory chain.
10. Protein transport, vesicular transport
Membrane-protein interactions, folding of membrane proteins.
Last update: T_FUUK (28.04.2008)
1. Lipidová (bio)membrána. Transport přes membránu - ionty, nepolární vs. polární molekuly, makromolekuly. Nespecifická permeace, aktivní a pasivní transport. Jejich termodynamické a kinetické aspekty. Membránový potenciál.
2. Metody studia transportu přes membránu. Pasivní transport - nespecifická permeace, usnadněná difúze. Kanály, transportéry. Oligopeptidy jako model studia membránových proteinů.
3. Struktura proteinů (se zaměřením na membránové), metody jejího studia
