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Interactions of biological macromolecules - NBCM135

Title:	Interakce biologických makromolekul
Guaranteed by:	Institute of Physics of Charles University (32-FUUK)
Faculty:	Faculty of Mathematics and Physics
Actual:	from 2008
Semester:	winter
E-Credits:	3
Hours per week, examination:	winter s.:2/0, Ex [HT]
Capacity:	unlimited
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Teaching methods:	full-time

Guarantor:	RNDr. Eduard Brynda, CSc.

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Annotation -

Last update: RNDr. Pavel Zakouřil, Ph.D. (09.06.2008)

Introduction to biochemistry for students of physics and technical sciences focused on applications interdisciplinary applications in biotechnology and medicine. Water, proteins, lipids, polysaccharides, nucleic acids. Intramolecular and intermolecular interactions of biological macromolecules. Biospecific interactions, such as, receptor-ligand, antibody-antigen, enzyme-substrate, complementarity of oligonucleotides. Affinity biochemical methods, affinity biosensors. Interaction of synthetic materials and objects with biological environment. Nanotechnology.

Literature -

Last update: RNDr. Pavel Zakouřil, Ph.D. (09.06.2008)

Biochemistry, D.Voet, J.G.Voet, John Wiley & Sons, 1990

Teaching methods -

Last update: RNDr. Pavel Zakouřil, Ph.D. (09.06.2008)

Lecture

Syllabus -

Last update: RNDr. Pavel Zakouřil, Ph.D. (09.06.2008)

Aqueous solutions - physical properties of water, ions, pH, buffers. Amino acids, Primary and three-dimensional protein structures. Physical forces controlling protein structure and non-covalent interactions of proteins with biological systems and synthetic surfaces. Acid and basic properties of proteins, hydrophobic interaction. Lipids, micelles, and bilayers of amphiphilic molecules, cell membranes. Structure of nucleic acids and stabilizing physical forces, replication, transcription, and translation. Enzymatic catalysis, substrate specificity, inhibition. Antibodies and antigens, membrane receptors, ligands. Affinity chromatography, immuno-analysis, receptors for biosensors, DNA and protein chips. Interface between synthetic surface and biological media, protein adsorption, interaction with blood, interaction with cells. Nanobiotechnologies, current trends.