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Biochemistry - NBCM012

Title:	Biochemie
Guaranteed by:	Institute of Physics of Charles University (32-FUUK)
Faculty:	Faculty of Mathematics and Physics
Actual:	from 2023
Semester:	summer
E-Credits:	3
Hours per week, examination:	summer s.:2/0, Ex [HT]
Capacity:	unlimited
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Teaching methods:	full-time

Guarantor:	Mgr. Dita Strachotová, Ph.D. RNDr. Kateřina Hofbauerová, Ph.D.
Classification:	Physics > Biophysics and Chemical Physics
Pre-requisite :	NBCM010
Is incompatible with:	NBCX012
Is pre-requisite for:	NBCM318, NBCM008
Is interchangeable with:	NBCX012

Opinion survey results Examination dates SS schedule Noticeboard

Annotation -

Last update: prof. RNDr. Marek Procházka, Ph.D. (30.04.2019)

Basic metabolic pathways (biological oxidation, metabolism of sugars, lipids, proteins, photosynthesis, citric acid cycle, regulation of metabolic processes).

Aim of the course -

Last update: GASKOVA/MFF.CUNI.CZ (14.05.2008)

Introduction in Biochemistry

Course completion requirements -

Last update: doc. RNDr. Dana Gášková, CSc. (30.10.2019)

Oral exam, exam requirements - in the extent of the syllabus.

Literature -

Last update: RNDr. Kateřina Hofbauerová, Ph.D. (14.12.2023)

D.Voet, J.G.Voet: Fundamentals of Biochemistry Life at the molecular LeveL, 5th, Wiley, 2016.

D.L.Nelson, M.M.Cox: Lehninger Principles of Biochemistry, 8th, Macmillan Learning, 2021.

H.R.Horton, L.A.Moran, K.G.Scrimgeour, M.D. Perry, J.D. Rawn: Principles of Biochemistry, 5th, Pearson Education, Inc., 2011.

J.L.Tymoczko, J.M.Berg, L.Stryer: Biochemistry, 9th, W. H. Freeman, 2019.

T.M.Devlin: Textbook of Biochemistry with Clinical Correlations, 7th, John Wiley & Sons, 2010.

Teaching methods -

Last update: GASKOVA/MFF.CUNI.CZ (14.05.2008)

basic course

Requirements to the exam -

Last update: doc. RNDr. Dana Gášková, CSc. (31.10.2019)

Oral exam requirements are within the scope of the lecture syllabus.

Syllabus -

Last update: T_FUUK (24.05.2004)

History of biochemistry. Introduction to metabolism: thermodynamics of living systems, metabolic pathways, experimental approaches to metabolic studies, thermodynamics of phosphate compounds, oxidation-reduction reactions.

Saccharide metabolism. Glycolysis:The glycolytic pathway, Glycolytic reactions, Fermentation ? anaerobic pyruvate degradation, Regulation of metabolic flux, Metabolism of other hexoses.

Other pathways of saccharide metabolism. Gluconeogenesis, Glyoxylate pathway, Biosynthesis of oligosaccharides and glycoproteins, Pentose cycle.

Citrate cycle. Overview, Metabolic sources of acetyl-coenzyme A, Citrate cycle enzymes, Citrate cycle regulation, Amphibolic nature of the citrate cycle.

Membrane transport.Transport thermodynamics, Transport kinetics and mechanisms, Primary active transport, Secondary active transport.

Electron transport and oxidative phosphorylation. Mitochondria, Electron transport, Oxidative phosphorylation, Regulation of ATP production.

Photosynthesis. Chloroplasts, Light-dependent reactions, Reactions taking place without direct participation of light.

Expression and transfer of genetic information. Replication: DNA ? the carrier of genetic information, DNA replication: an overview, Replication enzymes. Transcription: Role of RNA in protein synthesis, RNA polymerase, Transcription regulation, Post-transcription modifications. Translation: Genetic code, Transfer RNA, Ribosomes, Translation regulation, Post-translation modifications.