Scattering and Microscopy Methods - MC260P127A

• Title: Scattering and Microscopy Methods
• Guaranteed by: Department of Physical and Macromolecular Chemistry (31-260)
• Faculty: Faculty of Science
• Actual: from 2023
• Semester: summer
• E-Credits: 3
• Examination process: summer s.:
• Hours per week, examination: summer s.:1/1, Ex [HT]
• Capacity: unlimited
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: not taught
• Language: English
• Is provided by: MC260P127
• Note: enabled for web enrollment
• Guarantor: prof. RNDr. Miroslav Štěpánek, Ph.D.
• Teacher(s): prof. RNDr. Pavel Matějíček, Ph.D.; Michal Mazur, Ph.D.; prof. RNDr. Miroslav Štěpánek, Ph.D.

Annotation

Last update: RNDr. Kateřina Ušelová, Ph.D. (08.02.2022)

Introduction to microscopic and scattering methods. The course combines lectures comprising both theoretical
principles of the methods and their instrumentation with practical courses.

Literature

Last update: RNDr. Kateřina Ušelová, Ph.D. (08.02.2022)

Egerton, R.F. Physical Principles of Electron Microscopy, Springer Verlag 2007

Meyer, E., Hug, H.J., Bennewitz, R. Scanning Probe Microscopy, Spinger Verlag 2004

Zemb, T., Lindner, P. (Eds.) Neutrons, X-rays and Light. Scattering Methods Applied to Soft Condensed Matter, North Holland 2002

Requirements to the exam

Last update: RNDr. Kateřina Ušelová, Ph.D. (08.02.2022)

Oral exam

Syllabus

Last update: RNDr. Kateřina Ušelová, Ph.D. (08.02.2022)

1. Electron microscopy

Basic principles of electron microscopy imaging, magnification, point resolution, contrast, chromatic and spherical aberration. Transmission and scanning electron microscopy, detection in SEM/STEM methods, interaction of electrons with the sample, EDS and EELS techniques. Sample preparation, cryogenic transmission electron microscopy

2. Scanning probe microscopy

Basic principles of SPM imaging, atomic force microscopy in contact, semicontact and noncontact mode, scanning tunelling microscopy

3. Scattering methods

Introduction to scattering theory, scattering vector, scattering length and scattering cross-section, form factor and structure factor. Analysis of scattering curves. Dynamic light scattering, intensity autocorrelation function, translational and rotational diffusion coefficient from DLS. Ligh, X-ray and neutron scattering measurements - radiation sources, detection and calibration.