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Laboratory Course on Separation Methods - MC230C16

Title:	Praktikum ze separačních metod
Czech title:	Praktikum ze separačních metod
Guaranteed by:	Department of Analytical Chemistry (31-230)
Faculty:	Faculty of Science
Actual:	from 2023
Semester:	both
E-Credits:	4
Hours per week, examination:	0/2, C [DS]
Capacity:	20
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	Czech
Level:	specialized
Note:	enabled for web enrollment priority enrollment if the course is part of the study plan you can enroll for the course in winter and in summer semester

Guarantor:	doc. RNDr. Tomáš Křížek, Ph.D.
Teacher(s):	prof. RNDr. Zuzana Bosáková, CSc. Anton Korban, Ph.D. RNDr. Petr Kozlík, Ph.D. doc. RNDr. Tomáš Křížek, Ph.D.

Opinion survey results Examination dates WS schedule SS schedule

Files		Comments	Added by
	HPLC Chirální separace2012.pdf		prof. RNDr. Zuzana Bosáková, CSc.

Annotation -

Last update: doc. RNDr. Tomáš Křížek, Ph.D. (09.01.2024)

The laboratory course on separation methods is focused on gaining theoretical knowledge as well as experimental skills in advanced analytical instrumentation. Students will solve four complex problems using high-performance liquid chromatography with tandem mass detection, gas chromatography with mass or flame ionization detection (student's choice), capillary zone electrophoresis, and chiral capillary electrophoresis.

After passing this course students will be able in capillary electrophoresis, liquid chromatography with tandem mass spectrometry, and gas chromatography with flame-ionization or mass detection:
Independently operate instruments and perform analyses on the basic user level with written instructions.
Evaluate and interpret the experimental records.
Draw appropriate conclusions from the results.
Create a concise report containing a description of the experimental work, results, and conclusions, without serious factual or formal errors.

Literature -

Last update: doc. RNDr. Karel Nesměrák, Ph.D. (28.10.2019)

Analytické separační metody, K. Štulík a kol., Karolinum, Praha, 2005.
Encyclopedia of Separation Science. M. Crooke, C. F. Poole (Eds.) Academic Press 2000
Chromatography today, C.F. Poole, S.K. Poole, Elsevier, Amsterdam, 1991.

Requirements to the exam -

Last update: doc. RNDr. Tomáš Křížek, Ph.D. (05.09.2023)

Participation in the practical exercises, submission of appropriate reports, examinations for individual tasks, and scoring minimum 60% in the final test.

Syllabus -

Last update: doc. RNDr. Tomáš Křížek, Ph.D. (05.09.2023)

1) Three amino acids will be separated using a cyclodextrin-based chiral selector by capillary electrophoresis. The migration order of individual enantiomers will be determined. Enantiomers will be quantified using the standard addition method.
2) An HPLC-MS-MS method for analysis of selected organic pollutants will be developed. Focus will be on the optimization of MS/MS detection.
3) This exercise comprises gas chromatography with flame ionization or mass spectrometry detection. Students can choose according to their interests.
4) Ammonium, sodium and potassium ions in urine are separated and determined using capillary electrophoresis.