Biology of the cell nucleus - MB151P141

• Title: Biology of the cell nucleus
• Czech title: Biologie buněčného jádra
• Guaranteed by: Department of Cell Biology (31-151)
• Faculty: Faculty of Science
• Actual: from 2023
• Semester: summer
• E-Credits: 3
• Examination process: summer s.:oral
• Hours per week, examination: summer s.:2/0, Ex [HT]
• Capacity: 20
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: not taught
• Language: English
• Explanation: 16.2.24 nastaven jako nevyu4ovan7
• Note: enabled for web enrollment
• Guarantor: Mgr. Peter Hoboth, Ph.D.; Mgr. Martin Sztacho, Ph.D.
• Teacher(s): Mgr. Peter Hoboth, Ph.D.; Mgr. Martin Sztacho, Ph.D.

Annotation

Last update: RNDr. Nataša Šebková, Ph.D. (06.06.2023)

Biology of the cell nucleus is a complex topic that currently experiences an exciting period marked by many fascinating discoveries. These discoveries were enabled by the development of novel experimental approaches. The objective of this course is to provide the most up to date fundamentals of the recent discoveries that changed our understanding of the functional organization of the cell nucleus in health and disease. Methodological and technological advances will be discussed together with the biological implications, which they facilitate. We will discuss how recent discoveries shed a new light on the spatiotemporal regulation of the gene expression and heredity, which are the processes central to maintain the life on earth and executed mainly by nuclear functions. Emphasis will be on the concepts of the functional dynamic organization of the gene expression.

Event format: The course will be divided into 6 comprehensive blocks and will be held in English. It is expected that participants have previous basic knowledge of the cell biology.

Requirements: Fluent written and oral English; basic knowledge of the cell biology.

Literature

Last update: RNDr. Nataša Šebková, Ph.D. (06.06.2023)

Literature: Pombo A., Hetzer M.W., Misteli T. (eds.): The Nucleus (second ed.) CSH Perspectives in Biology 2021

Requirements to the exam

Last update: RNDr. Nataša Šebková, Ph.D. (06.06.2023)

Exam: After completion of this course, students will understand the functional organization of the nuclear processes, which will be verified by written multiple-choice test.

Syllabus

Last update: RNDr. Nataša Šebková, Ph.D. (06.06.2023)

Syllabus:

·         Block 1: Historical overview and introduction (Martin Sztacho)
- Evolution of the cell nucleus
- Nuclear parameters, size, number, nr. of chromosomes
- Nuclear structure, envelope, chromatin, nucleoli
- Function, genetic information, principles of compartmentalization
- Nuclear division

·         Block 2: Nuclear periphery (Peter Hoboth)
- Nuclear lamina
- Methods to measure genome organization – ChiP, proximity labelling, Hi-C
- Nuclear pore complex
- Nuclear pore complex in transcriptional regulation
- Cellular functions of inner nuclear membrane proteins

·         Block 3: Principles of organization (Martin Sztacho)
- Mechanical forces in nuclear organization
- Liquid-liquid phase separation in chromatin
- Stochastic genome and its role in gene expression
- Properties and function of actin and actin filaments inside nucleus

·         Block 4: Chromatin (Martin Sztacho)
- Physical nature of chromatin
- Chromatin folding and nuclear organization
- 3D chromatin modeling
- Transcription factor dynamics
- RNA in shaping the nuclear organization
- Nuclear compartments

·         Block 5: Nuclear processes (Peter Hoboth)
- Space and time on the functional genome output
- DNA replication timing
- Imaging the organization of RNA processing

·         Block 6: Pathophysiological processes (Peter Hoboth)
- Viruses in the nucleus
- Laminopathies
- Shaping genome during development
- Epigenetic reprograming in development
- Dynamics of X chromosome inactivation
- Organization of pluripotent genome

Entry requirements

Last update: RNDr. Nataša Šebková, Ph.D. (06.06.2023)

It is expected that participants have previous basic knowledge of the cell biology and fluent written and oral english. It is assumed that the students aim to understand the functional organization of the nuclear processes.