From Molecules to Cells - MB151P135E

• Title: From Molecules to Cells
• Czech title: Od molekul k buňkám
• Guaranteed by: Department of Cell Biology (31-151)
• Faculty: Faculty of Science
• Actual: from 2023 to 2023
• Semester: winter
• E-Credits: 5
• Examination process: winter s.:combined
• Hours per week, examination: winter s.:3/2, C+Ex [HT]
• Capacity: unlimited
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Note: enabled for web enrollment
• Guarantor: RNDr. Lenka Libusová, Ph.D.
• Teacher(s): prof. RNDr. Jan Černý, Ph.D.; doc. RNDr. Petr Folk, CSc.; RNDr. Lenka Libusová, Ph.D.; RNDr. Martin Pospíšek, Ph.D.

Annotation

Last update: doc. RNDr. František Půta, CSc. (06.02.2022)

The course From molecules to cells provides a definition of life in the context of biochemistry and cell biology. It
covers the basics of molecular mechanisms behind the complexity, sustainability and heredity of the living
organisms including evolutionary principles and phenomenon of the origin of life. It covers the biology of
prokaryotes and eukaryotes emphasizing the interplay between the structure and function on the seminal topics
like biosynthesis and transformation of cellular components and organelles; cell growth and oncogenic
transformation; transport, receptors, and cell signaling; the cytoskeleton, the extracellular matrix, and cell
movements; chromatin structure and RNA synthesis. The course From molecules to cells set the stage for
follow-up courses, particularly From cells to organisms. The course is built from topical blocks (see Syllabus)
each of them consisting of lecture (3h) and workshop focused on primary literature as a Q&A session(1h).

Literature

Last update: RNDr. Nataša Šebková, Ph.D. (31.05.2022)

1. Molecular Biology of the Cell, 7th Edition, ISBN-13: 978-0393884821, 2022
2. Molecular Cell Biology, 9th Edition, ISBN-13: 978-1319208523, 2021
3. Cell Biology, 3rd Edition, ISBN-13: 978-0323341264, 2016
4. Fundamentals of Biochemistry: Life at the Molecular Level, 5th Edition, ISBN-13: 978-1118918401, 2016
5. Lehninger Principles of Biochemistry, Eighth Edition, ISBN-13: 978-1319228002, 2021
6. Cell Biology by Numbers - , 2015
7. Lecture notes
8. Pre-recorded lectures
9. Problem sets

For each topical block students will be provided with the study materials and problem sets and it is expected that students will critically review those materials before Q&A sessions.

Requirements to the exam

Last update: doc. RNDr. František Půta, CSc. (06.02.2022)

Final mark is based on the oral examination (67%) and results of tests taken during the course (33%). Oral examination takes place during the examination period and students must first obtain the evaluation for Q&A sessions and take-home exercises.

Syllabus

Last update: doc. RNDr. František Půta, CSc. (06.02.2022)

Definition of life

Origin of life

Sustainability of life - metabolism

Strategies of energetic metabolism Information molecules

From nucleic acid to protein - central dogma of molecular biology

Protein biology

Concept of conformation change - regulation of enzyme activity, molecular machines

Cytoskeleton and cell mobility

Vesicular trafficking

Cell signalling

Cell cycle and its regulation

E pluribus unum - general cell biology principles under specific conditions (in differentiated organism in particular cell types or separate taxa)