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Last update: Mgr. Klára Hlouchová, Ph.D. (17.02.2021)
2 assignments / homeworks final grade: 25+25% for assignments, 50% final exam |
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Last update: Mgr. Klára Hlouchová, Ph.D. (07.10.2020)
3. Heterologous expression systems (prokaryotic, yeast, insect, mammalian) - transfection, cultivation, disruption 4. Basics of amino acid/protein structure, protein purification techniques I (affinity tags, ion exchange, hydrophobic, size-exclusion) 5. Protein purification techniques II, protein analysis (purity, concentration, dispersity, function, structure) 6. Repetition/back-up 7. Cell-free synthesis 8. Creating Diversity / Libraries epPCR, degenerate oligo design, gene shuffling, library preparation 9. Expanded / Alternative Genetic Code codon reassignment 10. Screen vs Selection towards new function display techniques (Phage, ribosomal, mRNA/cDNA), compartments 11. Minimal cells genome reduction 12. Synth Bio metabolic engineering; gene circuits |