Biology - Laboratory - MB151C06E

• Title: Biology - Laboratory
• Czech title: Biologie -praktické cvičení
• Guaranteed by: Department of Cell Biology (31-151)
• Faculty: Faculty of Science
• Actual: from 2023 to 2023
• Semester: summer
• E-Credits: 5
• Examination process: summer s.:
• Hours per week, examination: summer s.:0/4, C [HT]
• Capacity: unlimited
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: not taught
• Language: English
• Note: enabled for web enrollment
• Guarantor: Mgr. Aleš Benda, Ph.D.

Annotation

Last update: doc. RNDr. František Půta, CSc. (18.02.2022)

The practical course Biology – Laboratory is a hands on complement to the Biological Techniques, where selected
methodologies are trained as case projects using advanced instruments located in Faculty of Science core
facilities. Most of the practical are intended to be organized in BIOCEV, where state of art facilities are located,
together with educational lab environment. Practical tasks will be typically performed using model organisms
including E. coli, S. cerevisiae or pombe, X. tropicalis or M. musculus as a problem-oriented exercise reflecting the
real scientific project – implemented with education of basic laboratory skills.

The list of covered techniques will correspond to the broad diversity of biological disciplines, with the emphasis to
apply state of art approaches. All practical tasks are given by active scientists - experts in a field they teach or core
facility specialists.

The course Biology – Laboratory forms the integral unit with Biological Techniques namely and in extenso with
three prerequisite courses (From molecules to cells, From cells to organisms and On the Evolution and Ecology).

The practical course (5 days) is built from topical blocks (see Syllabus) followed by the presentation workshop
focused on data mining strategies an effective presentation skills (1 day) and students own presentation of
acquired data in a poster and oral format (1 day conference-style).

Literature

Last update: RNDr. Nataša Šebková, Ph.D. (31.05.2022)

1. Understanding Light Microscopy (RMS - Royal Microscopical Society) 1st Edition, ISBN-13: 978-0470973752, 2019
2. Introduction to Light Microscopy: Tips and Tricks for Beginners, 1st ed. 2019 Edition, ISBN-13: 978-3030053925, 2019
3. Proteomics for Biological Discovery, 2nd Edition, ISBN-13: 978-1118279243, 2019
4. Genome Editing and Engineering: From TALENs, ZFNs and CRISPRs to Molecular Surgery, 1st Edition, ISBN-13: 978-1107170377, 2018
5. Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins, 4th Edition, 2002
6. Lecture notes
7. Pre-recorded lectures
8. Problem sets

Requirements to the exam

Last update: doc. RNDr. František Půta, CSc. (18.02.2022)

Final mark is based on the presentation of data (67%) and results taken during the course (33%). Presentation conference takes place at the end of the course and students must first obtain the evaluation from the practical sessions, workshops and take-home exercises.

Syllabus

Last update: doc. RNDr. František Půta, CSc. (18.02.2022)

Seeing is believing - visualisation of specimens using various microscopes (SEM, TEM, light-based including life cell imaging, superresolution and image analysis Genetic analysis - preparation of samples for sequencing, transcriptomics, and proteomics analysis. Protein and nucleic acid isolation, characterization of structure and function Bioinformatics is applied to sequencing, transcriptomics and proteomics data. Design of gene modification (of the selected model organism), gene modification following functional analysis. Phenogenomics analysis - case study using genetically modified mouse model. Introduction to histological techniques - application of fluorescent proteins and probes. Preparation of samples - cell suspensions and use of antibodies + hands on training on flow cytometer.