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Cell cycles and signalling - MB140P29

Title:	Buněčné cykly a signály
Czech title:	Buněčné cykly a signály
Guaranteed by:	Department of Genetics and Microbiology (31-140)
Faculty:	Faculty of Science
Actual:	from 2023 to 2023
Semester:	winter
E-Credits:	3
Examination process:	winter s.:written
Hours per week, examination:	winter s.:2/0, Ex [HT]
Capacity:	unlimited
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	not taught
Language:	Czech
Level:	specialized
Note:	enabled for web enrollment the course is taught as cyclical

Guarantor:	prof. RNDr. Zdena Palková, CSc.

Opinion survey results Examination dates Schedule

Annotation -

Last update: KONOP (29.04.2002)

The lecture is focused on basic principles regulating cell and life cycles, differentiation and some other cellular processes like chemotaxis, cell polarity establishment and others. Individual cellular processes are documented on model organisms, predominantly prokaryotic or eukaryotic microorganisms. In particular cases, analogous processes in higher eukaryots are emphasised. The course is recommended for students in 4th or 5th year of the study and requires the knowledge of molecular biology and genetic engineering.

Literature -

Last update: RNDr. Michaela Schierová, Ph.D. (24.10.2019)

Recommended reviews and other papers, continuously updated

Requirements to the exam -

Last update: RNDr. Michaela Schierová, Ph.D. (24.10.2019)

The exam is written. The content of the exam is the content of lectures, presentations and recommended papers.

Syllabus -

Last update: IRLICHA (12.05.2006)

Please note that the lectures are given in Czech language only.

1. Introduction

Cell cycles and life cycles, methods of cell cycle analysis.

2. Cell cycle of E. coli

Overlapping cycles, replication, role of membrane and cytoskelet, septum formation.

3. Sporulation of Bacillus subtilis

Sporulation phases, switch of vegetative cycle to sporulation, two-component regulatory system, temporal and spatial regulation of sporulation, role of membrane, sigma factors.

4. Differantiation of Streptomyces

Airy mycelium formation, role of extracellular signal molecules.

5. Cell cycle of Caulobacter crescentus

Asymmetric cell cycle, role of two-component regulatory system.

6. Myxobacteria – social behaviour

Gliding; model of gliding, rippling, cooperative nutrient degradation, fruiting body formation, role of extracellular signal molecules.

7. Cell cycle of yeasts

Cell components important during cell division. Regulation of mitosis (p34, cyclines, replication).

8. Life cycle of yeasts

Mating types of yeasts and their change, conjugation (mating), meiosis (sporulation), pseudohyphal growth, apoptosis.

Signal pathways participating in these processes and their regulation.

9. Yeast cell polarity

Polar growth of yeast. Different features of haploid and diploid cells. Septins and other cell polarity proteins. External factors affecting cell polarity.

10. Life cycle of Dictyostelium discoideum

Regulation of cell cycle, differentiation and spore formation.

11. Chemotaxis

Regulation of chemotaxis in E. coli.

12. More examples of “metazoan” behaviour of microorganisms.

Quorum sensing bacteria, biofilms, formation and organisation of microbial colonies.

References:

Reviews and journal papers, actualised every year