Viral Biotechnology - MB140C67

• Title: Viral Biotechnology
• Czech title: Virové biotechnologie
• Guaranteed by: Department of Genetics and Microbiology (31-140)
• Faculty: Faculty of Science
• Actual: from 2021 to 2021
• Semester: summer
• E-Credits: 3
• Examination process: summer s.:
• Hours per week, examination: summer s.:0/1, C [TS]
• Capacity: 12
• Min. number of students: 6
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Level: specialized
• Guarantor: RNDr. Lenka Horníková, Ph.D.
• Teacher(s): Mgr. Martin Fraiberk, Ph.D.; RNDr. Lenka Horníková, Ph.D.; RNDr. Hana Španielová, Ph.D.
• Class: Ultracentrifuga a 3 rotory; Gradientový PCR cycler; Původní předmět

Annotation

English

Last update: RNDr. Irena Lichá, CSc. (11.05.2021)

One-week practical course is designed to introduce students to the methods of viral biotechnology. Students gain
strong theoretical background about the basic principles of viral expression systems, their possibility of usage for
recombinant proteins expression or for production of virus-like particles and methods of their purification. Using
virus-like particles as a model, the students gain practical experience with the methods of production (work with
cell cultures and recombinant viruses) and purification (centrifugation in density gradients) of virus like particles.

Czech

Last update: RNDr. Irena Lichá, CSc. (11.05.2021)

Týdenní praktický kurz je určen k seznámení studentů s metodami virové biotechnologie. Studenti získají
teoretické základy o základních principech virových expresních systémů, jejich možnosti využití pro expresy
rekombinantních proteinů nebo pro produkci virových částic a metodách jejich izolace. S využitím virových částic
jako modelu získají studenti praktické zkušenosti s metodami produkce (práce s buněčnými kulturami a
rekombinantními viry) a izolace (centrifugace v gradientech hustoty) virových částic.

Requirements to the exam

Last update: RNDr. Lenka Horníková, Ph.D. (17.02.2022)

For passing the course, the following requirements must be fulfilled:

1.  Participate actively in the course; any unexcused absence may be reason for disqualification from the course.
2.  During the course, focus only on activities related to the course.
3.  Submit protocols and answers to control questions due to date specified. This text can be returned for revision only once after consultation, credit will not be granted in case of repeated deficiencies.

Entry requirements

Last update: RNDr. Lenka Horníková, Ph.D. (29.02.2024)

·         The course starts on Monday, xxxx at 9.00 am in BIOCEV building (Prumyslova 595; Vestec), laboratory L1-012. Meeting point is at reception at 9:00. Enrolled participants are allowed to enter the laboratory only in laboratory clothing and shoes.

·         Each participant must be familiar with the instruction manual for the course, which is published at https://dl2.cuni.cz/course/view.php?id=4575 within the course "Viral Biotechnology". Enrollment key: will be provided to participants by e-mail (after 25.2.2022)